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The concentration of lactate in relation to other components of bovine mammary secretion during premature regression and after resumption of milking

Published online by Cambridge University Press:  01 June 2009

R. I. Mackie ,
W. H. Giesecke ,
H. Lück  and
P. A. de Villiers
R. I. Mackie
Affiliation:
Veterinary Research Institute, Onderstepoort, 0110, Republic of South Africa
W. H. Giesecke
Affiliation:
Veterinary Research Institute, Onderstepoort, 0110, Republic of South Africa
H. Lück
Affiliation:
Animal and Dairy Science Research Institute, Irene, 1675, Republic of South Africa
P. A. de Villiers
Affiliation:
Animal and Dairy Science Research Institute, Irene, 1675, Republic of South Africa
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Summary

An experiment lasting 42 d was performed in 4 consecutive stages on 6 healthy Friesian cows during mid-lactation. Mean values for the different components of mammary secretion during normal lactation were established. Milking was then suspended on all quarters from d 1–14. The mean values for lactate increased 20- to 30-fold over the mean value for normal lactation. Over the same period the leucocyte count (polymorphonuclear leucocytes and lymphocytes) also increased, whereas the mean values for glucose and the estimated redox potential decreased. From d 15–28 milking was resumed on one half of the udder and from d 29–42 milking was also resumed on the other udder-half. When milking was resumed the above-mentioned changes were reversed, taking 5–7 d to reach values obtained during normal lactation in the udder-half which had not been milked for 14 d, but at least 14 d in the udderhalf which had not been milked for 28 d. The changes in the levels of lactate, glucose, leucocyte count and estimated redox potential are discussed in relation to changes in the secretory activity of the mammary gland (lactose, β-lactoglobulin and epithelial cell count), permeability changes of the mammary epithelium (Na+, K+ and serum albumin) and the immune defence mechanism in the udder (immunoglobulins). The results indicate that lactate is formed during anaerobic glycolysis by the leucocytes in the mammary secretion, most probably due to reduced blood flow to the udder and the accumulation of secretion in the gland and teat cisterns on cessation of milking.

Type
Original Articles
Information
Journal of Dairy Research , Volume 44 , Issue 2 , June 1977 , pp. 201 - 211
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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References

REFERENCES

American Public Health Association (1967). Standard Methods for the Examination of Dairy Products. 12th Edn.New York: A.P.H.A.Google Scholar
Baierlein, J. L. & Foster, J. M. (1968). Blood 32, 412.Google Scholar
Baldwin, R. L. & Cheng, W. (1969). Journal of Dairy Science 52, 523.CrossRefGoogle Scholar
Bergmeyier, H. U. (1970). Methods of Enzymatic Analysis. 2nd Edn.Weinheim: Verlag Chemie.Google Scholar
Butler, J. E. (1974). Lactation, vol III, p. 217. (Eds Larson, B. L. and Smith, V. R.). New York: Academic Press.Google Scholar
Cline, M. J. (1965). Physiological Reviews 45, 674.Google Scholar
Elkin, A. R. & Kuhn, N. J. (1975). Biochemical Journal 146, 273.CrossRefGoogle Scholar
Fleet, I. R., Goode, J. A., Hamon, M. H., Laurie, M. S., Linzell, J. L. & Peaker, M. (1975). Journal of Physiology 251, 763.CrossRefGoogle Scholar
Giesecke, W. H. & van Den Heever, L. W. (1974). Onderstepoort Journal of Veterinary Research 41,169.Google Scholar
Giesecke, W. H. & Viljoen, M. H. (1974). Onderstepoort Journal of Veterinary Research 41, 51.Google Scholar
Have, A. J. V. D. & Mulder, H. (1957). Netherlands Milk and Dairy Journal 11, 128.Google Scholar
Hawkins, R. A. & Williamson, D. H. (1972). Biochemical Journal 129, 1171.Google Scholar
Hohorst, H. J., Arese, P., Barthls, H., Stratmann, D. & Talke, H. (1965). Annals of the New York Academy of Sciences 119, 974.CrossRefGoogle Scholar
Holten, C. H. (1971). Lactic Acid, p. 10. Weinheim: Verlag Chemie.Google Scholar
Karnovsky, M. J. (1962). Physiological Reviews 42, 143.CrossRefGoogle Scholar
Lerche, M. (1966). Lehrbuch der tierärztlichen Milchüberwachung. Berlin: Parey.Google Scholar
Linzell, J. L. (1974). Lactation, vol I, p. 143. (Eds Larson, B. L. and Smith, V. R.). New York: Academic Press.Google Scholar
Linzell, J. L. & Peaker, M. (1974). Journal of Physiology 243, 129.CrossRefGoogle Scholar
Lück, H., Giesecke, W. H., de Villiers, P. A. & Mackie, R. I. (1976). South African Journal of Dairy Technology 8, 69.Google Scholar
Lück, H. & Smith, A. (1975 a). South African Journal of Dairy Technology 7, 27.Google Scholar
Lück, H. & Smith, A. (1975 b). South African Journal of Dairy Technology 7, 149.Google Scholar
Morr, C. V., Harper, W. J. & Gould, I. A. (1957). Journal of Dairy Science 40, 964.Google Scholar
Newbould, F. H. S. (1974). Lactation, vol II, p. 269. (Eds Larson, B. L. and Smith, V. R.). New York: Academic Press.Google Scholar
Peaker, M. (1975). Journal of Dairy Science 58, 1042.CrossRefGoogle Scholar
Reineccius, G. A., Kavanagh, T. E. & Keeney, P. G. (1970). Journal of Dairy Science 53, 1018.CrossRefGoogle Scholar
Schalm, O. W. (1962). A Syllabus on the Bovine Mammary Glands in Health and Disease. Davis, Calif.: University of California.Google Scholar
Schanbacher, F. L. & Smith, K. L. (1975). Journal of Dairy Science 58, 1048.CrossRefGoogle Scholar
Valentine, W. N. (1956). Progress in Haematology. (Ed. Tocantins, L. M.) 1, 293.Google Scholar
Waldschmidt, M. (1973). Journal of Dairy Research 40, 7.CrossRefGoogle Scholar
Welty, F. K., Smith, K. L. & Schanbacher, F. L. (1976). Journal of Dairy Science 59, 224.Google Scholar
Yang, Y. T. & Baldwin, R. L. (1975). Journal of Dairy Science 58, 337.Google Scholar