Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-15T19:09:34.410Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections

Published online by Cambridge University Press:  05 May 2015

Norma B Russi ,
Julia Maito ,
Silvina A Dieser ,
María S Renna ,
Marcelo L Signorini ,
Cecilia Camussone ,
Verónica E Neder ,
Martín Pol ,
Liliana Tirante  and
Liliana M Odierno
...Show all authors
Norma B Russi
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Julia Maito
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Silvina A Dieser
Affiliation:
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km601, (5804) Río Cuarto, Córdoba, Argentina
María S Renna
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina
Marcelo L Signorini
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Cecilia Camussone
Affiliation:
Consejo Nacional de Investigaciones Científicas y Técnicas, Avda. Rivadavia 1917, (1033) Ciudad Autónoma de Buenos Aires, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Verónica E Neder
Affiliation:
Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
Martín Pol
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Liliana Tirante
Affiliation:
Lactodiagnóstico Sur. Pedro Goyena 3199, (1636) Olivos, Buenos Aires, Argentina
Liliana M Odierno
Affiliation:
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km601, (5804) Río Cuarto, Córdoba, Argentina
Luis F Calvinho*
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe. Argentina. Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, Ruta 34, km 227. (2300) Rafaela, Santa Fe, Argentina
*
*For correspondence; e-mail: [email protected].
Get access Rights & Permissions [Opens in a new window]

Abstract

Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.

Keywords

Staphylococcus aureusintramammary infectionspenicillin resistanceblaZ
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 317 - 321
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aarestrup, FM & Jensen, NE 1998 Development of penicillin resistance among Staphylococcus aureus isolated from bovine mastitis in Denmark and other countries. Microbial Drug Resistance 4 247–256CrossRefGoogle ScholarPubMed
Barkema, HW, Schukken, YH & Zadoks, RN 2006 Invited review: the role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. Journal of Dairy Science 89 1877–1895CrossRefGoogle ScholarPubMed
Bergan, T, Bruun, JN, Digranes, A, Lingaas, E, Melby, KK & Sander, J 1997 Susceptibility testing of bacteria and fungi. Report from the Norwegian Working Group on Antibiotics. Scandinavian Journal of Infectious Diseases 103 (Suppl.) 1–36Google Scholar
CLSI (Clinical and Laboratory Standards Institute) 2008 Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Vol. 28, No. 8. 3rd edition. Approved standard, M31-A3. Wayne, PA, USA: Clinical Laboratory Standards InstituteGoogle Scholar
CLSI (Clinical and Laboratory Standards Institute) 2013 Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Vol. 28, No. 8. 4th editon. Approved standard, VET01-A4. Wayne, PA, USA: Clinical Laboratory Standards InstituteGoogle Scholar
De Oliveira, AP, Watts, JL, Salmon, SA & Aarestrup, FM 2000 Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Europe and the United States. Journal of Dairy Science 83 855–862CrossRefGoogle ScholarPubMed
Dieser, SA, Vissio, C, Lasagno, MC, Bogni, CI, Larriestra, AJ & Odierno, LM 2014 Prevalence of pathogens causing subclinical mastitis in Argentinean dairy herds. Pakistan Veterinary Journal 34 124–126Google Scholar
Gianneechini, RE, Concha, C & Franklin, A 2002 Antimicrobial Susceptibility of Udder Pathogens Isolated from Dairy Herds in the West Littoral Region of Uruguay. Acta Veterinaria Scandinavica 43 31–41CrossRefGoogle ScholarPubMed
Haveri, M, Suominen, S, Rantala, L, Honkanen-Buzalski, T & Pyörälä, S 2005 Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infections. Veterinary Microbiology 106 97–102CrossRefGoogle Scholar
Jarløv, JO & Rosdahl, VT 1986 Quantitative determination of β-lactamase production in Staphylococcus aureus strains compared to qualitative testing by a microbiological clover leaf test, a chromogenic cephalosporin test and a iodometric test. Acta Pathologica Microbiologica Scandinavica B 94 415–421CrossRefGoogle Scholar
Klement, E, Chaffer, M, Leitner, G, Shwimmer, A, Friedman, S, Saran, A & Shipgel, N 2005 Assessment of accuracy of disk diffusion tests for the determination of antimicrobial susceptibility of common bovine mastitis pathogens: a novel approach. Microbial Drug Resistance 11 342–350CrossRefGoogle ScholarPubMed
Livermore, DM 1995 Beta-Lactamases in laboratory and clinical resistance. Clinical Microbiology Reviews 8 557–584CrossRefGoogle ScholarPubMed
Martineau, F, Picard, FJ, Roy, PH, Ouellette, M & Bergeron, MG 1998 Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. Journal of Clinical Microbiology 36 618–623CrossRefGoogle ScholarPubMed
Metzler, CM, DeHaan, RM 1974 Susceptibility tests of anaerobic bacteria: statistical and clinical considerations. Journal of Infectious Diseases 130 588–594CrossRefGoogle ScholarPubMed
Oberhofer, TR & Towle, DW 1982 Evaluation of the rapid pencillinase paper strip test for detection of beta-lactamase. Journal of Clinical Microbiology 15 196–199CrossRefGoogle Scholar
Oliver, SP, Gonzalez, RN, Hogan, JS, Jayarao, BM & Owens, WE 2004 Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, 4th edition. Verona, WI, USA: National Mastitis CouncilGoogle Scholar
Olsen, JE, Christensen, H & Aarestrup, FM 2006 Diversity and evolution of blaZ from Staphylococcus aureus and coagulase-negative staphylococci. Journal of Antimicrobial Chemotherapy 57 450–460CrossRefGoogle ScholarPubMed
Pengov, A & Ceru, S 2003 Antimicrobial drug susceptibility of Staphylococcus aureus strains isolated from bovine and ovine mammary glands. Journal of Dairy Science 86 3157–3163CrossRefGoogle ScholarPubMed
Persson, Y, Nyman, A & Grönlund-Andersson, U 2011 Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Veterinaria Scandinavica 53 36–43CrossRefGoogle ScholarPubMed
Pitkälä, A, Salmikivi, L, Bredbacka, P, Myllyniemi, AL & Koskinen, MT 2007 Comparison of tests for detection of β-lactamase-producing Staphylococci. Journal of Clinical Microbiology 45 2031–2033CrossRefGoogle ScholarPubMed
Pospiech, A & Neumann, B 1995 A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends in Genetics 11 217–218CrossRefGoogle ScholarPubMed
Roberson, JR, Fox, LK, Hancock, DD & Besser, TE 1992 Evaluation of methods for differentiation of coagulase-positive Staphylococci. Journal of Clinical Microbiology 30 3217–3219CrossRefGoogle ScholarPubMed
Russi, NB, Bantar, C & Calvinho, LF 2008 Antimicrobial susceptibility of Staphylococcus aureus causing bovine mastitis in Argentine dairy herds. Revista Argentina de Microbiología 40 116–119Google ScholarPubMed
Schlegelova, J, Rysánek, D, Sedivá, I & Babák, V 2001 Comparison of methods for the determination of antimicrobial resistance in Staphylococcus aureus from bovine mastitis. Journal of Veterinary Medicine Series B 48 21–29CrossRefGoogle ScholarPubMed
Tenhagen, BA, Köster, G, Wallmann, J & Heuwieser, W 2006 Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Journal of Dairy Science 89 2542–2551CrossRefGoogle ScholarPubMed
Vesterholm-Nielsen, M, Olhom Larsen, M, Elmerdahl Olsen, J & Aarestrup, FM 1999 Occurrence of the blaZ gene in penicillin resistant Staphylococcus aureus isolated from bovine mastitis in Denmark. Acta Veterinaria Scandinavica 40 279–286CrossRefGoogle ScholarPubMed
Watts, JL & Salmon, SA 1997 Activity of selected antimicrobial agents against strains of Staphylococcus aureus isolated from bovine intramammary infection that produce β-lactamase. Journal of Dairy Science 80 788–791CrossRefGoogle ScholarPubMed
Zecconi, A, Calvinho, L & Fox, L 2006 Staphylococcus aureus intramammary infections. Bulletin of the International Dairy Federation. 408/2006. pp. 39. FIL-IDF. ISSN 0250–5118Google Scholar