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A comparison of biochemical and paper chromatographic methods for the identification of group D Streptococci from Cheddar cheese

Published online by Cambridge University Press:  01 June 2009

T. I. Steenson
Affiliation:
Nationl Institute for Research in Dairying, Shinfield, Reading
P. S. Robertson
Affiliation:
Nationl Institute for Research in Dairying, Shinfield, Reading

Summary

Forty-four strains of tributyrolytic group D streptococci isolated from Cheddar cheese were identified by physiological, biochemical and serological tests. They were also independently grouped using two-way paper chromatography in which patterns of ninhydrin positive spots were developed from 10% acetic acid extracts of the bacteria. The groupings obtained by the chromatographic and conventional techniques were then compared. Strains of Str. durans were well differentiated by chromatography from the other species of group D streptococci examined, but with the remainder less clear differentiation was obtained.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1961

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References

REFERENCES

Berridge, N. J., Cheesbman, G. C., Mattick, A. T. R., Bottazzi, V. & Sharpe, M. E. (1957). J. appl. Bact. 20, 195.CrossRefGoogle Scholar
Breed, R. S., Murray, E. G. D. & Smith, N. R. (1957). Bergey's Manual of Determinative Bacteriology, 7th ed.London: Baillifère, Tindall and Cox.Google Scholar
Cheeseman, G. C. (1960). J. Dairy Res. 27, 33.CrossRefGoogle Scholar
Cheeseman, G. C. & Berridge, N. J. (1959). J. appl. Bact. 22, 307.CrossRefGoogle Scholar
Cheeseman, G. C., Berridge, N. J., Mattick, A. T. R., Bottazzi, V. & Sharpe, M. E. (1957). J. appl. Bact. 20, 205.CrossRefGoogle Scholar
Cheeseman, G. C., Silva, M. T. & Sharpe, M. E. (1959). J. appl. Bact. 22, 294.CrossRefGoogle Scholar
Gregory, M. & Mabbitt, L. A. (1957). J. appl. Bact. 20, 226.CrossRefGoogle Scholar
Hannay, C. L. & Newland, L. G. (1950). J. Dairy Res. 17, 206.CrossRefGoogle Scholar
Levy, A. L. & Chung, D. (1953). Analyt. Chem. 25, 396.CrossRefGoogle Scholar
Long, H. F. & Hammer, B. W. (1936). Ia. Agric. Exp. Sta. Res. Bull. no. 206.Google Scholar
Long, H. F. & Hammer, B. W. (1937). J. Sci. Ia. St. Coll. 11, 343.Google Scholar
Mattick, A. T. R., Cheeseman, G. C., Berridge, N. J. & Bottazzi, V. (1956). J. appl. Bact. 19, 310.CrossRefGoogle Scholar
Naylor, J. & Sharpe, M. E. (1958). J. Dairy Res. 25, 92.CrossRefGoogle Scholar
Robertson, P. S. (1960 a). J. Dairy Res. 27, 1.CrossRefGoogle Scholar
Robertson, P. S. (1960 b). J. Dairy Res. 27, 161.CrossRefGoogle Scholar
Shattock, P. M. F. (1949). J. gen. Microbiol. 3, 80.CrossRefGoogle Scholar
Shattock, P. M. F. (1955). Ann. Inst. Pasteur, 7, 95.Google Scholar
Stadhouders, J. & Mulder, H. (1957). Ned. melk- en Zuiveltijdschr. 11, 164.Google Scholar
Wheater, D. M. (1955). J. gen. Microbiol. 12, 123.CrossRefGoogle Scholar