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Chemiluminescent determination of xanthine oxidase activity in milk

Published online by Cambridge University Press:  01 August 1999

STEFANO GIROTTI ,
SANDRO LODI ,
ELIDA FERRI ,
GRAZIELLA LASI ,
FABIANA FINI ,
SEVERINO GHINI  and
ROLANDO BUDINI
STEFANO GIROTTI
Affiliation:
Unità Complessa Scienze Chimiche, Radiochimiche e Metallurgiche, Università degli Studi di Bologna, Via San Donato 15, I-40127 Bologna, Italia
SANDRO LODI
Affiliation:
Granarolo-Felsinea SpA, Via Cadriano 27/2, I-40127 Bologna, Italia
ELIDA FERRI
Affiliation:
Unità Complessa Scienze Chimiche, Radiochimiche e Metallurgiche, Università degli Studi di Bologna, Via San Donato 15, I-40127 Bologna, Italia
GRAZIELLA LASI
Affiliation:
Granarolo-Felsinea SpA, Via Cadriano 27/2, I-40127 Bologna, Italia
FABIANA FINI
Affiliation:
Unità Complessa Scienze Chimiche, Radiochimiche e Metallurgiche, Università degli Studi di Bologna, Via San Donato 15, I-40127 Bologna, Italia
SEVERINO GHINI
Affiliation:
Unità Complessa Scienze Chimiche, Radiochimiche e Metallurgiche, Università degli Studi di Bologna, Via San Donato 15, I-40127 Bologna, Italia
ROLANDO BUDINI
Affiliation:
Granarolo-Felsinea SpA, Via Cadriano 27/2, I-40127 Bologna, Italia
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Abstract

A chemiluminescent method for determining xanthine oxidase (XOD) activity was developed and applied to the assay of milk enzyme activity using a photomultiplier luminometer. Various kinds of milk and cream samples were analysed for XOD content. In pasteurized milk, XOD activity depended on the fat content and in UHT milk it disappeared owing to the heat treatment. Milk sample preparation was very simple, requiring only homogenization at 40°C followed by a 1[ratio ]10 dilution with UHT (‘XOD-free’) milk. The assay was carried out at 25°C. The response obtained from XOD standard solutions in milk was linear from 0·1 to 500 enzyme units (U) l−1, but for the actual milk samples values ranged only from 1 to 135 U l−1. The detection limit at 2 SD was 0·1 U l−1 in milk, while in buffer it was 100 times lower. The intra-assay and interassay CV for XOD activity in milk were 6–12%.

Type
Research Article
Information
Journal of Dairy Research , Volume 66 , Issue 3 , August 1999 , pp. 441 - 448
Copyright
© Proprietors of Journal of Dairy Research 1999

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