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Chemical changes in ultra-heat-treated milk during storage: III. Methods for the estimation of lysine and sugar-lysine derivatives formed by the maillard reaction

Published online by Cambridge University Press:  01 June 2009

A. B. Möller ,
A. T. Andrews  and
G. C. Cheeseman
A. B. Möller
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
A. T. Andrews
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
G. C. Cheeseman
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
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Summary

Four chemical methods were used in a comparison of the measurement of the extent of modification of lysine residues in casein during storage of ultra-heat-treated (UHT) milk. Determination of homoarginine after reaction with o-methylisourea appeared to be the best measure of total lysine modification. Reduction with borohydride before acid hydrolysis prevented recovery of lysine from lactuloselysine (ε-N-deoxylactulosyl-L-lysine) and fructoselysine (ε-N-deoxyfructosyl-L-lysine). This procedure gave values for these derivatives which were in good agreement with those obtained from the determination of furosine (ε-N-(2-furoylmethyl)-L-lysine) in acid hydrolysates. Determination of lysine after acid hydrolysis showed that lysine loss was accounted for by lactuloselysine and fructoselysine formation in most of the samples.

Browning was most apparent in UHT milk stored at 37 °C for 3 years and in this sample only part of the lysine residues was accounted for as lactuloselysine and fructoselysine, thus indicating that lysine had become involved further in sugar degradation products.

Type
Original Articles
Information
Journal of Dairy Research , Volume 44 , Issue 2 , June 1977 , pp. 277 - 281
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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References

REFERENCES

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