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Characterisation of Staphylococcus aureus strains isolated from mastitis bovine milk in Argentina

Published online by Cambridge University Press:  22 February 2018

Mariela E Srednik ,
Valentine Usongo ,
Sarah Lépine ,
Xavier Janvier ,
Marie Archambault  and
Elida R Gentilini
Mariela E Srednik*
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Microbiología, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina
Valentine Usongo
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Sarah Lépine
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Xavier Janvier
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Marie Archambault
Affiliation:
Faculté de médecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St- Hyacinthe, Québec J2S 2M2, Canada
Elida R Gentilini
Affiliation:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Microbiología, Av. Chorroarín 280, C1427CWO, Buenos Aires, Argentina
*
*For correspondence; e-mail: [email protected]
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Abstract

The study reported in this Research Communication was conducted to characterise Staphylococcus aureus isolates recovered from mastitic bovine milk from dairy herds in Argentina. A total of 829 mastitic milk samples, both clinical and subclinical, were collected from 21 farms by veterinarians and submitted to the laboratory for testing from which 229 S. aureus isolates were recovered, an isolation rate of 28·1%. These isolates were tested for susceptibility to the antibiotics penicillin, erythromycin and clindamycin. Of the 229 isolates, 53 (23·1%) were resistant to penicillin, 31 (13·5%) to erythromycin and 28 (12·2%) to clindamycin. All isolates were negative for the mecA, mecC and pvl genes by PCR. Southernblot hybridisation revealed that the ermC gene was located on plasmid bands. Eighty isolates were randomly selected from the 229 for further characterisation. Restriction analysis of chromosomal DNA with Cf9I followed by PFGE of the 80 isolates revealed 23 distinct pulsotypes at 80% similarity. Seven major types (A, B, N, P, S, T, U and V) accounted for 68·7% of these isolates and 12 pulsotypes (A, B, F, G, J, K, M, N, P, S, T and U) occurred on more than one farm indicating genetic diversity within the farms. MLST of a representative isolate from dominant types identified the STs 97 705, 746, 2102 and 2187 with ST97 being the most predominant. Antibiotic susceptibility testing showed that 53·7% of the 80 randomly selected isolates were resistant to at least one of the three antibiotics tested. To our knowledge, this study represents the first large scale molecular studies on S. aureus isolates from dairy farms in Argentina.

Keywords

Staphylococcus aureusbovine mastitisantimicrobial resistancemolecular typing
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 1 , February 2018 , pp. 57 - 63
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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Footnotes

†

These authors contributed equally to the work.

References

Bagcigil, AF, Taponen, S, Koort, J, Bengtsson, B, Myllyniemi, AL & Pyorala, S 2012 Genetic basis of penicillin resistance of S. aureus isolated in bovine mastitis. Acta Veterinaria Scandinavica 54 69 Google Scholar
Barkema, HW, Schukken, YH & Zadoks, RN 2006 Invited review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. Journal of Dairy Science 89 1877–1895 Google Scholar
Brakstad, OG, Aasbakk, K & Maeland, JA 1992 Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Journal of Clinical Microbiology 30 1654–1660 CrossRefGoogle ScholarPubMed
Clinical and Laboratory Standards Institute (CLSI) 2013 Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard Fourth Edition and Supplement VET01-A4 and VET01-S2 (Replaces M31 A3). Wayne, PA: Clinical Laboratory Standard Institute Google Scholar
El Haddad, L, Ben Abdallah, N, Plante, PL, Dumaresq, J, Katsarava, R, Labrie, S, Corbeil, J, St-Gelais, D & Moineau, S 2014 Improving the safety of Staphylococcus aureus polyvalent phages by their production on a Staphylococcus xylosus strain. PLoS ONE 9 e102600 CrossRefGoogle ScholarPubMed
Enright, MC, Day, NP, Davies, CE, Peacock, SJ & Spratt, BG 2000 Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. Journal of Clinical Microbiology 38 1008–1015 Google Scholar
Fessler, A, Scott, C, Kadlec, K, Ehricht, R, Monecke, S, Schwarz, S 2010 Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis. Journal of Antimicrobial Chemotherapy 65 619–625 Google Scholar
Haran, KP, Godden, SM, Boxrud, D, Jawahir, S, Bender, JB, Sreevatsan, S 2012 Prevalence and characterization of Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus, isolated from bulk tank milk from Minnesota dairy farms. Journal of Clininical Microbiology 50 688–695 Google Scholar
Hata, E, Katsuda, K, Kobayashi, H, Uchida, I, Tanaka, K & Eguchi, M 2010 Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin-resistant isolates from humans. Journal of Clinical Microbiology 48 2130–2139 Google Scholar
Kadlec, K, Wendlandt, S, Feβler, AT & Schwarz, S 2015 Methods for the detection of antimicrobial resistance and the characterization of Staphylococcus aureus isolates from food-producing animals and food of animal origin. Antimicrobial Resistance and Food Safety 45 207–232 Google Scholar
Kumar, R, Yadav, BR & Singh, RS 2010 Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Current Microbiology 60 379–386 Google Scholar
Li, J, Zhou, H, Yuan, L, He, T, Hu, S 2009 Prevalence, genetic diversity, and antimicrobial susceptibility profiles of staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China. Journal of Zhejiang University Science B 10 753–760 Google Scholar
Mulvey, MR, Chui, L, Ismail, J, Louie, L, Murphy, C, Chang, N, Alfa, M & Canadian Committee for the Standardization of Molecular Methods 2001 Development of a Canadian standardized protocol for subtyping methicillin-resistant Staphylococcus aureus using pulsed-field gel electrophoresis. Journal of Clinical Microbiology 39 3481–3485 Google Scholar
Neder, VE, Canavesio, VR & Calvinho, LF 2011 Presence of enterotoxigenic Staphylococcus aureus in bulk tank milk from Argentine dairy farms. Revista Argentina de Microbiologia 43 104–106 Google Scholar
Schmidt, T, Kock, MM & Ehlers, MM 2017 Characterization of Staphylococcus aureus isolated from bovine mastitis and close human contacts in South African dairy herds: genetic diversity and inter-species host transmission. Frontiers in Microbiology 8 511 CrossRefGoogle ScholarPubMed
Smith, EM, Green, LE, Medley, GF, Bird, HE, Fox, LK, Schukken, YH, Kruze, JV, Bradley, AJ, Zadoks, RN & Dowson, CG 2005 Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates. Journal of Clinical Microbiology 43 4737–4743 Google Scholar
Tenhagen, BA, Koster, G, Wallmann, J & Heuwieser, W 2006 Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Journal of Dairy Science 89 2542–2551 Google Scholar
Tenover, FC, Arbeit, RD, Goering, RV, Mickelsen, PA, Murray, BE, Persing, DH & Swaminathan, B 1995 Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of Clinical Microbiology 33 2233–2239 Google Scholar
Torres-Sangiao, E, Perez-Castro, S, Fernandez-Natal, MI, Cisterna-Cancer, R, Zapico-Gonzalez, M, Fernandez-Perez, B, Ojeda-Fernandez, E, Nebreda, T, Gozalo-Marguello, M, Fuster-Foz, C, Roiz-Mesones, MP, Miguel-Martin, MD, Torroba, L, Coira-Nieto, A, Vasallo-Vidal, F, Mendez-Lage, S, Prieto-Rodriguez, E, Eiros, JM, Torres, J, Bou, G 2012 Identification of international circulating lineages of meticillin-resistant Staphylococcus aureus in the north of Spain and their glycopeptide and linezolid susceptibility. Journal of Medical Microbiology 61 305–307 Google Scholar
Vintov, J, Aarestrup, FM, Zinn, CE & Olsen, JE 2003 Association between phage types and antimicrobial resistance among bovine Staphylococcus aureus from 10 countries. Veterinary Microbiology 95 133–147 Google Scholar
Wang, D, Wang, Z, Yan, Z, Wu, J, Ali, T, Li, J, Lv, Y & Han, B 2015 Bovine mastitis Staphylococcus aureus: antibiotic susceptibility profile, resistance genes and molecular typing of methicillin-resistant and methicillin-sensitive strains in China. Infection, Genetics and Evolution 31 9–16 Google Scholar
Wang, Y, Wu, CM, Lu, LM, Ren, GW, Cao, XY & Shen, JZ 2008 Macrolide-lincosamide-resistant phenotypes and genotypes of Staphylococcus aureus isolated from bovine clinical mastitis. Veterinary Microbiology 130 118–125 Google Scholar
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