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CD49f+ mammary epithelial cells decrease in milk from dairy cows stressed by overstocking during the dry period

Published online by Cambridge University Press:  20 November 2017

Mario Baratta ,
Silvia Miretti ,
Paolo Accornero ,
Giovanna Galeati ,
Andrea Formigoni ,
Gianfranco Gabai ,
Daniele Nucera  and
Eugenio Martignani
Mario Baratta*
Affiliation:
Department Veterinary Science, University of Turin, Italy, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Silvia Miretti
Affiliation:
Department Veterinary Science, University of Turin, Italy, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Paolo Accornero
Affiliation:
Department Veterinary Science, University of Turin, Italy, Largo Braccini 2, 10095 Grugliasco (TO), Italy
Giovanna Galeati
Affiliation:
Department Veterinary Medical Sciences (DIMEVET), University of Bologna, 40062 Ozzano dell'Emilia (BO), Italy
Andrea Formigoni
Affiliation:
Department Veterinary Medical Sciences (DIMEVET), University of Bologna, 40062 Ozzano dell'Emilia (BO), Italy
Gianfranco Gabai
Affiliation:
Department Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro (PD), Italy
Daniele Nucera
Affiliation:
Department Agricultural, Forest and Food Sciences, University of Torino, 10095 Grugliasco (TO), Italy
Eugenio Martignani
Affiliation:
Department Veterinary Science, University of Turin, Italy, Largo Braccini 2, 10095 Grugliasco (TO), Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

The work reported in this Research Communication describes the modification in epithelial cell populations during the first and the last month of milking in Holstein Friesian cows that have undergone different management during the dry period, and we report the differential expression of CD49f+ and cytokeratin18+ cell subpopulations. Twenty six cows were randomly divided into 2 balanced groups that were housed at stocking density of either 11 m2 (CTR) or 5 m2 from 21 ± 3 d before the expected calving until calving. Cells collected from milk samples taken in early lactation and late lactation were directly analysed for CD45, CD49f, cytokeratin 14, cytokeratin 18 and cell viability. We observed a differential expression with a significant reduction in CD49f+ (P < 0·01) and cytokeratin 18+ (P < 0·05) cells in early lactation. Differences were still evident in late lactation but were not significant. These observations suggest that mammary epithelial cell immunophenotypes could be associated with different animal management in the dry period and we hypothesise they may have a role as biomarkers for mammary gland function in dairy cows.

Keywords

integrin alpha 6stressbiomarkerdairy cow
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 4 , November 2017 , pp. 414 - 417
Copyright
Copyright © Hannah Research Foundation 2017 

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References

Baratta, M, Volpe, MG, Nucera, D, Gabai, G, Guzzo, N, Fustini, M & Martignani, E 2015 Differential expression of living mammary epithelial cell subpopulations in milk during lactation in dairy cows. Journal of Dairy Science 98 6897–6904 CrossRefGoogle ScholarPubMed
Bayer, J, Grunwald, D, Lambert, C, Mayol, JF & Maynadie, M 2007 Thematic workshop on fluorescence compensation settings in multicolor flow cytometry. Cytometry B, Clinical Cytometry 72 8–13 Google Scholar
Deugnier, MA, Faraldo, MM, Rousselle, P, Thiery, JP & Glukhova, MA 1999 Cell-extracellular matrix interactions and EGF are important regulators of the basal mammary epithelial cell phenotype. Journal of Cell Science 112 1035–1044 Google Scholar
Fustini, M, Galeati, G, Gabai, G, Mammi, LE, Bucci, D, Baratta, M, Accorsi, PA & Formigoni, A 2017 Overstocking dairy cows during the dry period affects dehydroepiandrosterone and cortisol secretion. Journal of Dairy Science 100 620–628 Google Scholar
Gabai, G, Marinelli, L, Simontacchi, C & Bono, GG 2004 The increase in plasma C19Delta5 steroids in subcutaneous abdominal and jugular veins of dairy cattle during pregnancy is unrelated to estrogenic activity. Steroids 69 121–7Google Scholar
Garbe, JC, Pepin, F, Pelissier, FA, Sputova, K, Fridriksdottir, AJ, Guo, DE, Villadsen, R, Park, M, Petersen, OW, Borowsky, AD, Stampfer, MR & Labarge, MA 2012 Accumulation of multipotent progenitors with a basal differentiation bias during aging of human mammary epithelia. Cancer Research 72 3687–3701 CrossRefGoogle ScholarPubMed
LaBarge, MA, Nelson, CM, Villadsen, R, Fridriksdottir, A, Ruth, JR, Stampfer, MR, Petersen, OW & Bissell, MJ 2009 Human mammary progenitor cell fate decisions are products of interactions with combinatorial microenvironments. Integrative Biology 1 70–79 CrossRefGoogle ScholarPubMed
Martignani, E, Cravero, D, Miretti, S, Accornero, P & Baratta, M 2015 Clonogenic assay allows for selection of a primitive mammary epithelial cell population in bovine. Experimental Cell Research 338 245–250 Google Scholar
Prunier, A, Mounier, L, Le, NP, Leterrier, C, Mormede, P, Paulmier, V, Prunet, P, Terlouw, C & Guatteo, R 2013 Identifying and monitoring pain in farm animals: a review. Animal 7 998–1010 CrossRefGoogle ScholarPubMed
Rupp, R, Beaudeau, F & Boichard, D 2000 Relationship between milk somatic-cell counts in the first lactation and clinical mastitis occurrence in the second lactation of French Holstein cows. Preventive Veterinary Medicine 46 99–111 Google Scholar
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