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Catabolism of L-phenylalanine by some microorganisms of cheese origin

Published online by Cambridge University Press:  01 June 2009

Chang-Won Lee  and
Jean Richard
Chang-Won Lee
Affiliation:
Laboratoire de Microbiologie Laitière, Institut National de la Recherche Agronomique, CNRZ 78350, Jouy-en-Josas, France
Jean Richard
Affiliation:
Laboratoire de Microbiologie Laitière, Institut National de la Recherche Agronomique, CNRZ 78350, Jouy-en-Josas, France
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Summary

The ability of 42 microorganisms isolated from cheese, including strains of yeasts, Geotrichum candidum, Arthrobacter, Moraxella spp., Brevibacterium linens and Staphylococcus saprophyticus spp. to produce phenethyl alcohol (PEA) from L-phenylalanine was studied. All the yeast strains produced labelled PEA from L-[U-14C]phenylalanine. Phenylpyruvic acid was detected as an intermediate of PEA production and CO2 was produced by decarboxylation of this acid. For five strains tested, the level of PEA which had accumulated in the culture at the end of exponential growth phase represented 39–52% of the L-Phe added. None of G. candidum strains nor bacterial isolates produced PEA from L-Phe. Strains of Moraxella spp. and four strains of the S. saprophyticus group produced phenylacetaldehyde. For three strains of Arthrobacter spp., five of B. linens and all the Moraxella, 14CO2 produced from uniformly labelled L-Phe represented more than one carbon atom of the L-Phe molecule, suggesting that Phe was catabolized beyond the stage of phenylacetic acid. Production and disappearance of PEA during Camembert cheese ripening is probably related to the metabolic activity of microorganisms present at the cheese surface.

Type
Original Articles
Information
Journal of Dairy Research , Volume 51 , Issue 3 , August 1984 , pp. 461 - 469
Copyright
Copyright © Proprietors of Journal of Dairy Research 1984

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References

REFERENCES

Adda, J., Roger, S. & Dumont, J. P. 1978 Some recent advances in the knowledge of cheese flavor. In Flavor of Foods and Beverages pp. 65–74. (Eds Charalambous, G. and Inglett, G. E.). New York: Academic PressCrossRefGoogle Scholar
Asakawa, T., Wada, H. & Yamano, T. 1968 Enzymatic conversion of phenylpyruvate to phenylacetate. Biochimica et Biophysica Acta 170 375–391CrossRefGoogle ScholarPubMed
Äyräpää, T. 1965 The formation of phenethyl alcohol from 14C-labeled phenylalanine. Journal of the Institute of Brewing 71 341–347CrossRefGoogle Scholar
Chen, T. C. & Levin, R. E. 1974 Taxonomic significance of phenethyl alcohol production by Achromobacter isolates from fishery sources. Applied and Environmental Microbiology 28 681–687CrossRefGoogle ScholarPubMed
Dumont, J. P., Adda, J. & Rousseaux, P. 1981 [An example of flavour variation within the same type of cheese: Gruyère de Comté.] Lebensmittel-Wissenschaft und Technologie 14 198–202Google Scholar
Dumont, J. P., Roger, S., Cerf, O. & Adda, J. 1974 [Study of the neutral volatile compounds in Camembert cheese.] Lait 54 501–516CrossRefGoogle Scholar
Hrabák, A. & Ferenczi, S. 1971 Thin layer ion-exchange chromatography on resin-coated chromatoplates. IV. Determination of ornithine in biological fluids. Acta Biochimica et Biophysica Acadeviiae Scientiarum Hungaricae 6 383–384Google ScholarPubMed
Kishore, G., Sugumaran, M. & Vaidyanathan, C. S. 1976 Metabolism of DL-(±)-phenylalanine by Aspergillus niger. Journal of Bacteriology 128 182–191CrossRefGoogle ScholarPubMed
Kloos, W. E. & Wolfshohl, J. F. 1982 Identification of Staphylococcus species with the Api Staph-Ident System. Journal of Clinical Microbiology 16 509–516CrossRefGoogle ScholarPubMed
Lamparsky, D. & Klimes, I. 1981 Cheddar cheese flavour – its formation in the light of new analytical results. In Flavour ′81 pp. 557–577. (Ed. Schreier, P.). Berlin: W. de GruyterCrossRefGoogle Scholar
Marusich, W. C., Jensen, R. A. & Zamir, L. O. 1981 Induction of phenylalanine ammonia-lyase during utilisation of phenylalanine as a carbon or nitrogen source in Rhodotorula glutinis. Journal of Bacteriology 146 1013–1019CrossRefGoogle ScholarPubMed
Moutounet, M. 1969 [Biosynthesis of higher alcohols in fermented drinks.] Annales de Technologie Agricole 18 249–261Google Scholar
Richard, J. & Zadi, H. 1983 [List of the dominant bacterial species in Camembert cheeses made with raw milk.] Lait 63 25–42CrossRefGoogle Scholar
Salo, P. 1970 Determining the odor thresholds for some compounds in alcoholic beverages. Journal of Food Science 35 95–99CrossRefGoogle Scholar
Sapis, J. C. 1967 [Study in wines of tyrosol, tryptophol, phenylethanol and γ-butyrolactone, secondary products of alcoholic fermentation.] Thesis, Bordeaux University.Google Scholar
Schmidt, J. L. & Lenoir, J. 1978 [The yeast flora of Camembert cheese. Its development during ripening.] Lait 58 355–370CrossRefGoogle Scholar
S., Senthe Shanmuganathan & Elsden, S. R. 1958 The mechanism of the formation of tyrosol by Saccharomyces cerevisiae. Biochemical Journal 69 210–218Google Scholar
Wright, E. A. & Levin, R. E. 1976 The mechanism of phenylethanol production from L-phenylalanine by Moraxella-like isolates from refrigerated foods. Abstracts of the annual meeting of the American Society for Microbiology P8 p. 188Google Scholar
Wright, E. A. & Levin, R. E. 1977 Significance of phenethyl alcohol production from L-phenylalanine by psychrotrophic Moraxella-like isolates from refrigerated foods. Abstracts of the annual meeting of the American Society for Microbiology. P14 p. 257Google Scholar