Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-12-03T15:00:36.542Z Has data issue: false hasContentIssue false
  • English
  • Français

Carbohydrate analysis of the glycopeptides released by the action of rennin on whole milk

Published online by Cambridge University Press:  01 June 2009

G. Sinkinson  and
J. V. Wheelock
G. Sinkinson
Affiliation:
School of Biological Sciences, The University, Bradford 7
J. V. Wheelock
Affiliation:
School of Biological Sciences, The University, Bradford 7
Get access Rights & Permissions [Opens in a new window]

Summary

A gas-liquid chromatographic method is described for the simultaneous estimation of the carbohydrates attached to the glycopeptides released by the action of rennin on whole milk. The method can also be used for κ-casein. Certain of the findings may be applicable to other glycoproteins or glycopeptides. In particular: (a) the conditions for methanolysis of the carbohydrates from the glycopeptides must be chosen carefully to avoid decomposition of N-acetyl neuraminic acid; (b) it is not possible to obtain a satisfactory calibration for N-acetyl neuraminic acid using the free acid as a standard.

Type
Original Articles
Information
Journal of Dairy Research , Volume 37 , Issue 1 , February 1970 , pp. 113 - 120
Copyright
Copyright © Proprietors of Journal of Dairy Research 1970

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Blix, G., Lindberg, E., Odin, L. & Werner, I. (1956). Acta Soc. Med. upsal. 61, 1.Google Scholar
Bolton, C. H., Clamp, J. R., Dawson, G. & Hough, L. (1965). Carbohydrate Res. 1, 333.CrossRefGoogle Scholar
Clamp, J. R., Dawson, G. & Hough, L. (1967). Biochim. biophys. Acta 148, 342.CrossRefGoogle Scholar
Jollès, P., Alais, C. & Jollès, J. (1961). Biochim. biophys. Acta 51, 309.CrossRefGoogle Scholar
Karkas, J. D. & Chargaff, E. (1964). J. biol. Chem. 239, 949.Google Scholar
Mackinlay, A. G. & Wake, R. G. (1965). Biochim. biophys. Acta 104, 167.CrossRefGoogle Scholar
Neuberger, A. & Marshall, R. D. (1966). In Glycoproteins. (Ed. Gottschalk, A..) Amsterdam, London and New York: Elsevier Publishing Company.Google Scholar
Sweeley, C. C., Bentley, R., Makita, M. & Wells, W. W. (1963). J. Am. chem. Soc. 85, 2497.CrossRefGoogle Scholar
Wake, R. G. (1959). Aust. J. biol. Sci. 12, 479.Google Scholar
Warren, L. (1959). J. biol. Chem. 234, 1971.CrossRefGoogle Scholar
Waugh, D. F. & Hippel, P. H. von (1956). J. Am. chem. Soc. 78, 4576.CrossRefGoogle Scholar