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Carbohydrate analysis of the glycopeptides released by the action of rennin on whole milk

Published online by Cambridge University Press:  01 June 2009

G. Sinkinson  and
J. V. Wheelock
G. Sinkinson
Affiliation:
School of Biological Sciences, The University, Bradford 7
J. V. Wheelock
Affiliation:
School of Biological Sciences, The University, Bradford 7
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Summary

A gas-liquid chromatographic method is described for the simultaneous estimation of the carbohydrates attached to the glycopeptides released by the action of rennin on whole milk. The method can also be used for κ-casein. Certain of the findings may be applicable to other glycoproteins or glycopeptides. In particular: (a) the conditions for methanolysis of the carbohydrates from the glycopeptides must be chosen carefully to avoid decomposition of N-acetyl neuraminic acid; (b) it is not possible to obtain a satisfactory calibration for N-acetyl neuraminic acid using the free acid as a standard.

Type
Original Articles
Information
Journal of Dairy Research , Volume 37 , Issue 1 , February 1970 , pp. 113 - 120
Copyright
Copyright © Proprietors of Journal of Dairy Research 1970

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References

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