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Automatic method to quantify starter activity based on pH measurement

Published online by Cambridge University Press:  01 June 2009

Henry E. Spinnler  and
Georges Corrieu
Henry E. Spinnler
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, France
Georges Corrieu
Affiliation:
Laboratoire de Génie des Procédés Biotechnologiques Agro-Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, France
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Summary

A new method for measuring the activity of mesophilic and thermophilic lactic acid bacteria is proposed based on measuring the pH of cultures at extremely short intervals (30–90 s) during growth and calculating several kinetic parameters, i.e. the maximum acidification rate (Vm), the time and pH at which Vm occurred, the time or pH range during which the observed rates were greater than Vm/2. These were easily calculated by coupling the pH meter to a microcomputer.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 755 - 764
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

REFERENCES

Accolas, J. P. & Auclair, J. 1970 [Determination of the acid-producing activity of concentrated frozen suspensions of lactic acid bacteria.] Lait 50 609–626CrossRefGoogle Scholar
Accolas, J. P., Veaux, M. & Auclair, J. 1971 [Study of the interactions between various thermopbilie and mesopbilic lactic acid bacteria in relation to the manufacture of Gruyère cheese.] Lait 51 249–272CrossRefGoogle Scholar
Bautista, E. S., Dahiva, R. S. & Speck, M. L. 1966 Identification of compounds causing symbiotic growth of Streptococcus thermophilus and Lactobacillus bulgaricus in milk. Journal of Dairy Research 33 297–307CrossRefGoogle Scholar
Bracquart, P. & Lorient, D. 1977 [The effect of amino acids on the growth of Streptococcus thermophilus.] Milchwissenschaft 32 221–224Google Scholar
De Soet, J. J. & De Graaff, J. 1988 An improved method with computer registration of fermentation activities of microorganisms. Journal of Fermentation Technology 66 239–242CrossRefGoogle Scholar
Famelart, M. H. & Maubois, J. L. 1988 [Comparison of refractive index and viscosity evolution during acid coagulation of milk.] Lait 68 1–12CrossRefGoogle Scholar
Galesloot, T. E., Hassing, F. & Veringa, H. A. 1968 Symbiosis in yoghurt. I. Stimulation of Lactobacillus bulgaricus by a factor produced by Streptococcus thermophilus. Netherlands Milk and Dairy Journal 22 50–63Google Scholar
Heap, H. A. & Lawrence, R. C. 1981 Recent modifications to the New Zealand activity test for Cheddar cheese starters. New Zealand Journal of Dairy Science and Technology 16 91–94Google Scholar
Horral, B. E. & Elliker, P. R. 1947 An activity test for Cheddar and cottage cheese starters. Journal of Dairy Science 30 523–524Google Scholar
Juillard, V., Desmazeaud, M. J. & Spinnler, H. E. 1988 [Demonstration of urease activity in Streptococcus thermophilus.] Canadian Journal of Microbiology 34 818–822CrossRefGoogle Scholar
Juillard, V., Spinnler, H. E., Desmazeaud, M. J. & Boquien, C. Y. 1987 [Cooperation and inhibition between lactic acid bacteria used in the dairy industry.] Lait 67 149–172CrossRefGoogle Scholar
Lamprech, E. D. & Foster, E. M. 1963 The survival of starter organisms in concentrated suspensions. Journal of Applied Bacteriology 26 359–369CrossRefGoogle Scholar
Okigbo, L. M., Oberg, C. J. & Richardson, G. H. 1985 Lactic culture activity tests using pH and impedance instrumentation. Journal of Dairy Science 68 2521–2526CrossRefGoogle Scholar
Radke-Mitchell, L. C. & Sandine, W. E. 1986 Influence of temperature on associative growth of Streptococcus thermophilus and Lactobacillus bulgaricus. Journal of Dairy Science 69 2558–2568CrossRefGoogle ScholarPubMed
Spinnler, H. E., Bouillanne, C., Desmazeaud, M. J. & Corrieu, G. 1987 Measurement of the partial pressure of dissolved CO2 for estimating the concentration of Streptococcus thermophilus in coculture with Lactobacillus bulgaricus. Applied Microbiology and Biotechnology 25 464–470CrossRefGoogle Scholar
Spinnler, H. E., Jomier, Y., Corrieu, G. & Chabanet, C. 1989 [Statistical analysis of measures enabling the viability and activity of lactic starters to be estimated.] Industries Agricoles et Alimentaires (submitted for publication).Google Scholar
Tinson, W., Broome, M. C., Hillier, A. J. & Jago, G. R. 1982 Metabolism of Streptococcus thermophilus. 2. Production of CO2 and NH3 from urea. Australian Journal of Dairy Technology 37 14–16Google Scholar