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Association of bovine αs1- and β-casein using the fluorescent properties of 1,8-anilinonaphthalene sulphonate

Published online by Cambridge University Press:  01 June 2009

L. K. Creamer  and
J. V. Wheelock
L. K. Creamer
Affiliation:
New Zealand Dairy Research Institute, Palmerston North, New Zealand
J. V. Wheelock
Affiliation:
School of Biological Sciences, University of Bradford, Bradford, England
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Summary

The effects of NaCl, CaCl2 and temperature on the emitted fluorescence of anilinonaphthalene sulphonate (ANS) in solutions of αs1- and β-casein indicated that the ANS was associated with the protein. Increased aggregation of the protein was responsible for further enhancement of the fluorescence. Addition of CaCl2 to a mixture of αs1- and β-casein at 37 °C caused a greater increase in fluorescence than when αs1- or β-casein was used alone, supporting the suggestion that these 2 proteins form co-polymers with different properties from the homopolymers and indicating that hydrophobic bonding between αs1- and β-casein may be important in the presence of Ca.

Type
Research Article
Information
Journal of Dairy Research , Volume 42 , Issue 3 , October 1975 , pp. 381 - 389
Copyright
Copyright © Proprietors of Journal of Dairy Research 1975

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References

REFERENCES

Ainsworth, S. & Flanagan, M. T. (1969). Biochimica et Biophysica Acta 194, 213.CrossRefGoogle Scholar
Barel, A. O., Turneer, M. & Dolmans, M. (1972). European Journal of Biochemistry 30, 26.CrossRefGoogle Scholar
Berry, G. P. & Creamer, L. K. (1975). Biochemistry (in the press).Google Scholar
Brand, L. & Gohlke, J. R. (1972). Annual Review of Biochemistry 41, 843.CrossRefGoogle Scholar
Clarke, R. F. L. & Nakai, S. (1972). Biochimica et Biophysica Acta 257, 70.CrossRefGoogle Scholar
Creamer, L. K. & Berry, G. P. (1975). Journal of Dairy Research 42, 169.CrossRefGoogle Scholar
Dalgleish, D. G. (1973). European Journal of Biochemistry 40, 375.CrossRefGoogle Scholar
Edelman, G. M. & McClure, W. O. (1968). Accounts of Chemical Research 1, 65.CrossRefGoogle Scholar
Gally, J. A. & Edelman, G. M. (1964). Biopolymers Symposium 1, 367.Google Scholar
Ho, C. & Chen, A. H. (1967). Journal of Biological Chemistry 242, 551.CrossRefGoogle Scholar
Lawrence, R. C. & Creamer, L. K. (1969). Journal of Dairy Research 36, 11.CrossRefGoogle Scholar
Payens, T. A. J. & Van Markwijk, B. W. (1963). Biochimica et Biophysica Acta 71, 517.CrossRefGoogle Scholar
Payens, T. A. J. & Nijhuis, H. (1974). Biochimica et Biophysica Acta 336, 201.CrossRefGoogle Scholar
Pearce, K. N. (1975). European Journal of Biochemistry (in the press).Google Scholar
Pearce, K. N. & Creamer, L. K. (1974). Analytical Chemistry 46, 457.CrossRefGoogle Scholar
Penzer, G. R. (1972). European Journal of Biochemistry 25, 218.CrossRefGoogle Scholar
Pepper, L. (1972). Biochimica et Biophysica Acta 278, 147.CrossRefGoogle Scholar
Schmidt, D. G. (1970). Biochimica et Biophysica Acta 207, 130.CrossRefGoogle Scholar
Schmidt, D. G. & Payens, T. A. J. (1972). Journal of Colloid and Interface Science 39, 655.CrossRefGoogle Scholar
Stryer, L. (1968). Science 162, 526.CrossRefGoogle Scholar
Sullivan, R. A., Fitzpatrick, M. M., Stanton, E. K., Annino, R., Kissel, G. & Palermiti, F. (1955). Archives of Biochemistry and Biophysics 55, 455.CrossRefGoogle Scholar
Swaisgood, H. E. & Timasheff, S. N. (1968). Archives of Biochemistry and Biophysics 125, 344.CrossRefGoogle Scholar
Waugh, D. F. (1971). In Milk Proteins 2, 3. (Ed. McKenzie, H. A..) New York: Academic Press.CrossRefGoogle Scholar
Waugh, D. F., Creamer, L. K., Slattery, C. W. & Dresdner, G. W. (1970). Biochemistry 9, 786.CrossRefGoogle Scholar
Waugh, D. F. & Noble, R. W. (1965). Journal of the American Chemical Society 87, 2246.CrossRefGoogle Scholar
Waugh, D. F., Slattery, C. W. & Creamer, L. K. (1971). Biochemistry 10, 817.CrossRefGoogle Scholar