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Association of bovine CD4 and STAT5b single nucleotide polymorphisms with somatic cell scores and milk production traits in Chinese Holsteins

Published online by Cambridge University Press:  25 March 2011

Yanghua He ,
Qin Chu ,
Peipei Ma ,
Yachun Wang ,
Qin Zhang ,
Dongxiao Sun ,
Yi Zhang ,
Ying Yu  and
Yuan Zhang
Yanghua He
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Qin Chu
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, 100097, Beijing, P.R. China
Peipei Ma
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Yachun Wang
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Qin Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Dongxiao Sun
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Yi Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Ying Yu*
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
Yuan Zhang*
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, P.R. China
*
*For correspondence: [email protected];[email protected]
*For correspondence: [email protected];[email protected]
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Abstract

CD4+ T cells play a key role in the immune response of pathogen-induced mastitis in dairy cattle. Mammary gland factor STAT5b is involved in the regulation of CD4+T cell differentiation during inflammatory response and milk production. Little is known about the genetic variation effects of bovine CD4 and STAT5b genes on somatic cell score (SCS) and milk production traits in dairy cattle. The aim of the study was to investigate the single nucleotide polymorphisms (SNPs) of bovine CD4 and STAT5b in Chinese Holsteins and to analyse their association with estimated breeding values (EBVs) for SCS and milk production traits. In the present study, SNPs of CD4 (NC_007303 g.13598C>T) and STAT5b (NC_007317 g.31562 T>C) were identified and genotyped in Chinese Holstein population. The results showed that both SNPs were significantly associated with the EBVs for milk yield and protein yield in Chinese Holstein cows, and the SNP in CD4 was associated with the EBV for SCS (P<0·01). The additive effect of CD4 SNP on protein yield was significant (P<0·05), and the dominant effect of STAT5b SNP was significant on milk yield and protein yield (P<0·01). Cows with combination genotype C7 (CCTT: CD4 g.13598C>T and STAT5b g.31562 T>C) had the highest SCS EBV but lower milk yield, while cows with C2 (TTTC) produced more milk, fat and protein than the other eight combination genotypes. These results suggested that the SNPs in CD4 and STAT5b may be potential genetic markers for SCS and milk/protein yields selecting and warrant further functional research.

Keywords

CD4STAT5bsingle nucleotide polymorphismssomatic cell scoremilk production traitsChinese Holstein
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 2 , May 2011 , pp. 242 - 249
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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References

Ahn, J, Yu, K, Stolzenberg-Solomon, R, Simon, KC, McCullough, ML, Gallicchio, L, Jacobs, EJ, Ascherio, A, Helzlsouer, K, Jacobs, KB, Li, Q, Weinstein, SJ, Purdue, M, Virtamo, J, Horst, R, Wheeler, W, Chanock, S, Hunter, DJ, Hayes, RB, Kraft, P & Albanes, D 2010 Genome-wide association study of circulating vitamin D levels. Human Molecular Genetics 19 2739–2745Google Scholar
Arenas, M, Fairbanks, LD, Vijayakumar, K, Carr, L, Escuredo, E & Marinaki, AM 2009 An unusual genetic variant in the MOCS1 gene leads to complete missplicing of an alternatively spliced exon in a patient with molybdenum cofactor deficiency. Journal of Inherited Metabolic Diseases 32 560–569CrossRefGoogle Scholar
Bansal, A, van den Boom, D, Kammerer, S, Honisch, C, Adam, G, Cantor, CR, Kleyn, P & Braun, A 2002 Association testing by DNA pooling: an effective initial screen. Proceedings of the National Academy of Sciences of the USA 99 16871–16874Google Scholar
Darnell, JE Jr 1997 STATs and gene regulation. Science 277 1630–1635CrossRefGoogle ScholarPubMed
Falconer, DS & Mackay, TFC 1996 Introduciton to Quantitiative Genetics, 4th Edition. New York, USA: Longman Scientific and TechnicalGoogle Scholar
Fedorova, L & Fedorov, A 2003 Introns in gene evolution. Genetica 118 123–131CrossRefGoogle ScholarPubMed
Gao, J, Liu, XQ, Chen, LB, Shi, WM, Su, JL & Han, B 2010 Epidemiological survey about prevalence of bovine mastitis in Beijing region. Highlights of Science Papers Online 3 1180–1185Google Scholar
Glazov, EA, Kongsuwan, K, Assavalapsakul, W, Horwood, PF, Mitter, N & Mahony, TJ 2009 Repertoire of bovine miRNA and miRNA-like small regulatory RNAs expressed upon viral infection. PLoS One 4 e6349CrossRefGoogle ScholarPubMed
Hagnestam-Nielsen, C, Emanuelson, U, Berglund, B & Strandberg, E 2009 Relationship between somatic cell count and milk yield in different stages of lactation. Journal of Dairy Science 92 3124–3133CrossRefGoogle ScholarPubMed
He, F, Sun, DX, Yu, Y, Wang, YC & Zhang, Y 2007 SNPs detection of STAT5A and association with milk production traits in Holstein cattle. Acta Veterinaria et Zootechnica Sinica 38 326–331Google Scholar
Hill, WL 2010 Understanding and using quantitative genetic variation. Philosphical Transactions of the Royal Society of London, B Biological Sciences 365 73–85CrossRefGoogle ScholarPubMed
Holmberg, M & Andersson-Eklund, L 2004 Quantitative trait loci affecting health traits in Swedish dairy cattle. Journal of Dairy Science 87 2653–2659Google Scholar
Hu, HC, Wang, HM, Li, JB, Wang, CF, Lai, SJ, Li, QL & Zhong, JF 2009 Genetic polymorphism of Nramp1 gene and correlation with mastitis in Holstein cattle. Hereditas 31 57–2Google ScholarPubMed
Igaz, P, Tóth, S & Falus, A 2001 Biological and clinical significance of the JAK-STAT pathway: lessons from knockout mice. Inflammation Disease 50 435–441Google Scholar
Imada, K, Bloom, ET, Nakajima, H, Horvath-Arcidianoco, JA & Udy, GB 1998 Stat5b is essential for natural killer cell-mediated proliferation and cytolytic activity. Journal of Experimental Medicine 188 2067–2074CrossRefGoogle ScholarPubMed
Jiang, L, Liu, JF, Sun, DX, Ma, PP, Ding, XD, Yu, Y & Zhang, Q 2010 Genome wide association studies for milk production traits in Chinese Holstein population. Plos One 5 e13661CrossRefGoogle ScholarPubMed
Ke, X, Collins, A & Ye, S 2001 PIRA-PCR designer for restriction analysis of single nucleotide polymorphisms. Bioinformatics 17 838–839Google Scholar
Keightley, PD & Halligan, DL 2009 Analysis and implications of mutational variation. Genetica 136 359–369CrossRefGoogle ScholarPubMed
Khatib, H, Monson, RL, Schutzkus, V, Kohl, DM, Rosa, GJM & Rutledge, JJ 2008 Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle. Journal of Dairy Science 91 784–793CrossRefGoogle ScholarPubMed
Leyva-Baca, I, Schenkel, F, Martin, J & Karrow, NA 2008 Polymorphisms in the 5′ upstream region of the CXCR1 chemokine receptor genes, and their association with somatic cell score in Holstein cattle in Canada. Journal of Dairy Science 91 407–417CrossRefGoogle ScholarPubMed
Li, S & Rosen, JM 1995 Nuclear factor 1 and mammary gland factor (STAT5) play a critical role in regulating rat whey acidic protein gene expression in transgenic mice. Molecular and Celluar Biology 15 2063–2070Google Scholar
Liu, X, Robinson, GW, Gouilleux, F, Groner, B & Hennighausen, L 1995 Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue. Proceedings of the National Academy of Sciences of the USA 92 8831–8835CrossRefGoogle Scholar
Madsen, P & Jensen, J 2007 A user's Guide to DMU, version 6, release 4.7, University of Aarhus, Faculty of Agricultural Sciences, Denmark.Google Scholar
Madsen, P, Sørensen, P & Su, G 2006 DMU—a package for analysing multivariate mixed models. In: Book of Abstracts, Proceedings CD of the 8th World Congress on Genetics Applied to Livestock Production, 13–18. Belo Horizonte, Brazil.Google Scholar
Mark, T, Fikse, F, Banos, G, Emanuelson, U & Philipsson, J 2000 Summary of mace pilot-runs for somatic cell count and clinical mastitis. Interbull Bulletin 26 43–52Google Scholar
Mark, T, Fikse, WF, Emanuelson, U & Philipsson, J 2002 International genetic evaluations of Holstein sires for milk somatic cells and clinical mastitis. Journal of Dairy Science 85 2384–2392Google Scholar
Miglior, F, Gong, W, Wang, Y, Kistemaker, GJ, Sewalem, A & Jamrozik, J 2009 Short communication: Genetic parameters of production traits in Chinese Holsteins using a random regression test-day model. Journal of Dairy Science 92 4697–4706Google Scholar
Moriggl, R, Topham, DJ, Teglund, S, Sexl, V, McKay, C & Wang, D 1999 STAT5 is required for IL-2-induced cell cycle progression of peripheral T cells. Immunity 10 249–259CrossRefGoogle ScholarPubMed
Mohammed, KA, Nasreen, N, Ward, MJ & Antony, VB 2000 Induction of acute pleural inflammation by Staphylococcus aureus. I. T cells play a critical role in experimental empyema. Journal of Infectious Diseases 181 1693–1699CrossRefGoogle Scholar
Oyugi, JO, Vouriot, FC, Alimonti, J, Wayne, S, Luo, M, Land, AM, Ao, Z, Yao, X, Sekaly, RP, Elliott, LJ, Simonsen, JN, Ball, TB, Jaoko, W, Kimani, J, Plummer, FA & Fowke, KR 2009 A common CD4 gene variant is associated with an increased risk of HIV-1 infection in Kenyan female commercial sex workers. Journal of Infectious Diseases 199 1327–1334Google Scholar
Rivas, AL, Schwager, SJ, Gonzále, RN, Quimby, FW & Anderson, KL 2007 Multifactorial relationships between intramammary invasion by Staphylococcus aureus and bovine leukocyte markers. Canadian Journal of Veterinary Research 71 135–144Google Scholar
Ron, M, Feldmesser, E, Golik, M, Tager-Cohen, I, Kliger, D, Reiss, V, Domochovsky, R, Alus, O, Seroussi, E, Ezra, E & Weller, JI 2004 A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. Journal of Dairy Science 87 476–490CrossRefGoogle ScholarPubMed
Rothschild, M, Jacobson, C, Vaske, D, Tuggle, C, Wang, L, Short, T, Eckardt, G, Sasaki, S, Vincent, A, McLaren, D, Southwood, O, van der Steen, H, Mileham, A & Plastow, G 1996 The estrogen receptor locus is associated with a major gene influencing litter size in pigs. Proceedings of the National Academy of Sci ences of theUSA 93 201–205Google Scholar
Rupp, R & Boichard, D 1999 Genetic parameters for clinical mastitis, somatic cell score, production, udder type traits, and milking ease in first lactation Holsteins. Journal of Dairy Science 82 2198–2204CrossRefGoogle ScholarPubMed
Rupp, R, Bergonier, D, Dion, S, Hygonenq, MC, Aurel, MR, Foulon, E & Foucras, G 2006 Effects of SCC-based selection for mastitis resistance: First results from a divergent selection experiment in sheep. In Eigthth world congress on genetics applies to livestock production Belo horizonte, MG, Brazil.Google Scholar
SAS Institute 2006 SAS OnlineDoc. Version 9.1. SAS Institute Inc., Cary NC, USAGoogle Scholar
Selvaggi, M, Dario, C, Normanno, G, Celano, GV & Dario, M 2009 Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle. Journal of Dairy Research 76 441–445Google Scholar
Shelley, S & Hill, AVS 2003 Genetic susceptibility to infectious disease. Trends in Microbiology 11 445–448Google Scholar
Soltys, J & Quinn, MT 1999 Selective recruitment of T-cell subsets to the udder during Staphylococcal and Streptococcal mastitis: analysis of lymphocyte subsets and adhesion molecule expression. Infection and Immunity 67 6293–6302CrossRefGoogle Scholar
Taylor, BC, Keefe, RG, Dellinger, JD, Nakamura, Y & Cullor, JS 1997 T cell populations and cytokine expression in milk derived from normal and bacteria-infected bovine mammary glands. Cell Immunology 182 68–76CrossRefGoogle ScholarPubMed
Wakao, H, Gouilleux, F & Groner, B 1994 Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO Journal 13 2182–2191Google Scholar
Wang, XP, Xu, SZ, Ma, TH, Gao, X, Ren, HY & Chen, JB 2006 Genetic variation in the 5′ flanking region of bovine TLR4 gene and correlation with mastitis. Hereditas 28 1520–1524Google ScholarPubMed
Wilson, CB, Rowell, E & Sekimata, M 2009 Epigenetic control of T helper-cell differentiation. Nature Reviews (Immunology) 9 91–105CrossRefGoogle ScholarPubMed
Viguier, C, Arora, S, Gilmartin, N, Welbeck, K & O'Kennedy, R 2009 Mastitis detection: current trends and future perspectives. Trends in Biotechnology 27 489–493Google Scholar