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Action of plasmin on bovine β-casein in a membrane reactor

Published online by Cambridge University Press:  01 June 2009

Servaas Visser ,
Henk J. Noorman ,
Charles J. Slangen  and
Harry S. Rollema
Servaas Visser
Affiliation:
Netherlands Institiite for Dairy Research (NIZO), P.O. Box 20, 6710 BA Ede, The Netherlands
Henk J. Noorman
Affiliation:
Netherlands Institiite for Dairy Research (NIZO), P.O. Box 20, 6710 BA Ede, The Netherlands
Charles J. Slangen
Affiliation:
Netherlands Institiite for Dairy Research (NIZO), P.O. Box 20, 6710 BA Ede, The Netherlands
Harry S. Rollema
Affiliation:
Netherlands Institiite for Dairy Research (NIZO), P.O. Box 20, 6710 BA Ede, The Netherlands
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Summary

The degradation of bovine β-casein by plasmin was used as a model system to investigate the applicability of an enzyme recycle reactor for the continuous production and isolation of peptide fractions. A simple, ultrafiltration-type membrane reactor was used incorporating a commercially available hollow-fiber module normally employed for dialysis purposes (artificial kidney). The results were compared with those obtained in a batch reactor process, using HPLC to monitor the course of proteolysis.

Peptide fragments were isolated and were characterized using amino-acid analysis and N-terminal end-group determination. Sixteen peptide fragments were identified, which together accounted for virtually all the potential cleavage sites in β-casein. In the system studied the N-terminal half of β-casein appeared to be more sensitive to plasmic hydrolysis than the rest of the molecule.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 3: Special Issue: Proceedings of the Hannah Research Institute Casein Conference , May 1989 , pp. 323 - 333
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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