Hostname: page-component-669899f699-ggqkh Total loading time: 0 Render date: 2025-04-26T22:32:12.636Z Has data issue: false hasContentIssue false
  • English
  • Français

Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows

Published online by Cambridge University Press:  08 August 2018

Mengqi Wang ,
Hailiang Song ,
Xiaorui Zhu ,
Shiyu Xing ,
Meirong Zhang ,
Huimin Zhang ,
Xiaolong Wang ,
Zhangping Yang ,
Xiangdong Ding  and
Niel A. Karrow
...Show all authors
Mengqi Wang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Hailiang Song
Affiliation:
Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Xiaorui Zhu
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Shiyu Xing
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Meirong Zhang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Huimin Zhang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Xiaolong Wang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Zhangping Yang
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China
Xiangdong Ding
Affiliation:
Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Niel A. Karrow
Affiliation:
Department of Animal Biosciences, Center for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, Canada
Sven König
Affiliation:
Institute of Animal Breeding and Genetics, Justus-Liebig-University Gießen, Ludwigstr. 21b, Gießen D-35390, German
Yongjiang Mao*
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China Department of Animal Biosciences, Center for Genetic Improvement of Livestock, University of Guelph, Guelph, Ontario N1G 2W1, Canada Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, P. R. China
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The research reported in this Research Communication aimed to describe the influence of Toll-like receptor 4 gene polymorphisms on milk production traits in Chinese Holstein cows. Toll-like receptor 4 (TLR4) is an important member of the toll-like receptor gene family that is widely found in various organisms. Since TLR4 can identify molecular patterns from various pathogenic microorganisms and induce natural and acquired immunity, it plays an important role in disease resistance in dairy cows. Two single nucleotide polymorphisms (SNPs) of TLR4 (c.−226 G > C and c.2021 C > T) that were previously found to be associated with health traits were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) for Chinese Holstein cows (n = 866). The associations between SNPs or their haplotypes and milk production traits and somatic cell count were analyzed by the generalized linear model procedure of Statistics Analysis System software (SAS). The c.−226 G > C and c.2021 C > T showed low linkage disequilibrium (r2 = 0·192). There was no association between these two SNPs and SCC, but significant effects were found for SNP c.−226 G > C on test-day milk yield, fat content, protein content, and total solid and milk urea nitrogen (P < 0·05), and SNP c.2021 C > T and the SNP haplotypes on test-day milk yield, fat content, protein content, lactose content and total solids (P < 0·05). The software MatInspector revealed that c.−226 G > C was located within several potential transcription factor binding sites, including transcription factor AP-2. The polymorphisms c.−226 G > C and c.2021 C > T had significant effects on the milk production for Chinese Holstein, and these SNP could be used for molecular marker-assisted selection of milk production.

Keywords

Toll-like receptor 4single nucleotide polymorphismsSomatic Cell Countmilk production traits
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 4 , November 2018 , pp. 407 - 411
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

Footnotes

†

These authors contributed equally to this work.

References

Beecher, C, Daly, M, Childs, S, Berry, DP, Magee, DA, McCarthy, TV & Giblin, L 2010 Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genetics 11 99Google Scholar
Beecher, C, Daly, M, Ross, RP, Flynn, J, McCarthy, TV & Giblin, L 2012 Characterization of the bovine innate immune response in milk somatic cells following intramammary infection with Streptococcus dysgalactiae subspecies dysgalactiae. Journal of Dairy Science 95 5720–5729Google Scholar
Cartharius, K, Frech, K, Grote, K, Klocke, B, Haltmeier, M, Klingenhoff, A, Frisch, M, Bayerlein, M & Werner, T 2005 Matinspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics 21 2933–2942Google Scholar
Hilger-Eversheim, K, Moser, M, Schorle, H & Buettner, R 2000 Regulatory roles of AP-2 transcription factors in vertebrate development, apoptosis and cell-cycle control. Gene 260 1–12Google Scholar
Hill, WG & Robertson, A 1968 Linkage disequilibrium in finite populations. Theoretical & Applied Genetics 38 226–231Google Scholar
Mishra, C, Kumar, S, Yathish, HM, Choudhary, R & Kumar, A 2016. Association of tlr4 gene variants with milk production traits in crossbred cattle. Agri Gene 1 69–71.Google Scholar
Miyake, K 2007 Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors. Seminars in Immunology 19 3–10Google Scholar
Ruiz-Larrañaga, O, Manzano, C, Iriondo, M, Garrido, JM, Molina, E, Vazquez, P, Juste, RA & Estonba, A 2011 Genetic variation of toll-like receptor genes and infection by Mycobacterium avium ssp. paratuberculosis in Holstein-Friesian cattle. Journal of Dairy Science 94 3635–3641Google Scholar
Rupp, R & Boichard, D 1999 Genetic parameters for clinical mastitis, somatic cell score, production, udder type and milking easy in first lactation Holsteins. Journal of Dairy Science 82 2198–2204Google Scholar
Sharma, BS, Mount, J & Karrow, NA 2008 Functional characterization of a single nucleotide polymorphism in the 5′ UTR region of the bovine toll-like receptor 4 gene. Developmental Biology 132 331–336Google Scholar
Sharma, BS, Abo-Ismail, MK, Schenkel, FS, You, Q, Verschoor, CP, Pant, SD, Karrow, NA 2015 Association of TLR4 polymorphisms with Mycobacterium avium subspecies paratuberculosis infection status in Canadian Holsteins. Animal Genetics 46 560–565Google Scholar
Shi, YY & He, L 2005 SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction & genetics association at polymorphism loci. Cell Research 15 97–98Google Scholar
Stephens, M, Smith, N & Donnelly, P 2001 A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics 68 978–989Google Scholar
Tian, W, Wu, T, Zhao, R, Xu, J, He, Y, & Wang, H 2017 Responses of milk production of dairy cows to jugular infusions of a mixture of essential amino acids with or without exclusion leucine or arginine. Animal Nutrition 3 271–275Google Scholar
Wang, WP, Xu, SZ, Gao, X, Li, JY, Ren, HY & Zhuo-ma, LR 2008 Cloning and SNP screening of the TLR4 gene and the association between its polymorphism and somatic cell score in dairy cattle. South African Journal of Animal Science 38 101–109Google Scholar
Wang, XP, Luoreng, ZM, Gao, SX, Guo, DS, Li, JY, Gao, X, Xu, SZ, Li, F, Chen, G & Wang, JR 2014 Haplotype analysis of TLR4 gene and its effects on milk somatic cell score in Chinese commercial cattle. Molecular Biology Reports 41 2345–2351Google Scholar
Wiggans, GR & Shook, GE 1987 A lactation measure of somatic cell count. Journal of Dairy Science 70 2666–2672Google Scholar
Winfrey, MR, Rott, MA & Wortman, AT 1997 Unraveling DNA: Molecular Biology for the Laboratory. Upper Saddle River, NJ, USA: Prentice-HallGoogle Scholar
Zhou, H, Cheng, L, Gong, H, Byun, SO, Edwards, GR, & Hickford, J 2017 Variation in the toll-like receptor 4 (tlr4) gene affects milk traits in dairy cows. Journal of Dairy Research 84 426–429Google Scholar
Supplementary material: PDF

Wang et al. supplementary material

Wang et al. supplementary material 1

Download Wang et al. supplementary material(PDF)
PDF 321.4 KB