Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-18T10:06:36.141Z Has data issue: false hasContentIssue false

The rebodying of stirred yoghurt: interactions between proteins

Published online by Cambridge University Press:  14 July 2008

Marie Renan
Affiliation:
Inra-Agrocampus Rennes, UMR1253, Science et Technologie du Lait et de l'Œuf, 65 rue de St Brieuc, F-35 042 Rennes cedex, France
Fanny Guyomarc'h
Affiliation:
Inra-Agrocampus Rennes, UMR1253, Science et Technologie du Lait et de l'Œuf, 65 rue de St Brieuc, F-35 042 Rennes cedex, France
Véronique Arnoult-Delest
Affiliation:
DANONE Research, RD 128, 91767 Palaiseau Cedex, France
Denis Pâquet
Affiliation:
DANONE Research, RD 128, 91767 Palaiseau Cedex, France
Gérard Brulé
Affiliation:
Inra-Agrocampus Rennes, UMR1253, Science et Technologie du Lait et de l'Œuf, 65 rue de St Brieuc, F-35 042 Rennes cedex, France
Marie H Famelart*
Affiliation:
DANONE Research, RD 128, 91767 Palaiseau Cedex, France
*
*For correspondence; e-mail: [email protected]

Abstract

The aim of the present study was to identify the nature of bonds established between protein particles after stirring that are responsible for the texture improvement of stirred yoghurts, called rebodying. Using a constant model yoghurt at pH 4·4, the effects of changes in the physicochemical conditions at stirring were studied on the subsequent rebodying. Short term rebodying was measured as the changes in viscoelastic properties at 4°C during 20 h after stirring, while long-term rebodying was measured as the viscosity changes during 28 d storage at 4°C. Moreover, stirred gels obtained from either set gels that were allowed time or not for ionic equilibration were compared. Increasing or decreasing ionic strength did not change the properties of stirred gels. Calcium chloride addition significantly decreased G′0 h, G′20 h and tan20 h but did not induce changes in the gel microstructure as observed by confocal scanning microscopy. Yoghurt rebodying could not be explained by fulfilling ionic equilibrium. Moreover, N-ethyl maleimide addition had no effect on the stirred yoghurt. Attractive electrostatic and disulphide interactions were not involved in the gel rebodying and increasing calcium concentration in the set gel limited rebodying.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Afonso, IM & Maia, JM 1999 Rheological monitoring of structure evolution and development in stirred yoghurt. Journal of Food Engineering 42 183190CrossRefGoogle Scholar
Alting, AC, Hamer, RJ, de Kruif, CG & Visschers, RW 2000 Formation of disulfide bonds in acid-induced gels of pre-heated whey proteins isolate. Journal of Agricultural and Food Chemistry 48 50015007CrossRefGoogle Scholar
Arshad, M, Paulsson, M & Dejmek, P 1993 Rheology of build-up, breakdown, and rebodying of acid casein gels. Journal of Dairy Science 76 33103316CrossRefGoogle Scholar
Brulé, G, Maubois, JL & Fauquant, J 1974 Etude de la teneur en éléments minéraux des produits obtenus lors de l'ultrafiltration du lait sur membrane. Le Lait 54 600615CrossRefGoogle Scholar
Cayot, P, Fairise, JF, Colas, B, Lorient, D & Brulé, G 2003 Improvement of rheological properties of firm acid gels by skim milk heating is conserved after stirring. Journal of Dairy Research 70 423431CrossRefGoogle ScholarPubMed
Cordeschi, M, Di Paola, L, Marrelli, L & Maschietti, M 2003 Net proton charge of beta- and kappa-casein in concentrated aqueous electrolyte solutions. Biophysical Chemistry 103 7788CrossRefGoogle ScholarPubMed
Holt, C, Dalgleish, DG & Jenness, R 1981 Calculation of the ion equilibria in milk diffusate and comparison with experiment. Analytical Biochemistry 113 154163CrossRefGoogle ScholarPubMed
International Dairy Federation 1987 Milk, cream and evaporated milk – Determination of total solids content. Standard 21B, pp. 2. Brussels Belgium: International Dairy FederationGoogle Scholar
Laligant, A, Famelart, MH, Brulé, G, Piot, M & Paquet, D 2003 Fermentation by lactic bacteria at two temperatures of pre-heated reconstituted milk. I – Behaviour of proteins and minerals. Le Lait 83 181192CrossRefGoogle Scholar
Lankes, H, Ozer, HB & Robinson, RK 1998 The effect of elevated milk solids and incubation temperature on the physical properties of natural yoghurt. Milchwissenschaft 53 510513Google Scholar
Le Graet, Y & Brulé, G 1993 Les équilibres minéraux du lait: Influence du pH et de la force ionique. Le Lait 73 5160CrossRefGoogle Scholar
Lee, WJ & Lucey, JA 2004 Structure and physical properties of yogurt gels: Effect of inoculation rate and incubation temperature. Journal of Dairy Science 87 31533164CrossRefGoogle ScholarPubMed
Lee, WJ & Lucey, JA 2006 Impact of gelation conditions and structural breakdown on the physical and sensory properties of stirred yogurts. Journal of Dairy Science 89 23742385CrossRefGoogle ScholarPubMed
Lefebvre-Cases, E, Gastaldi, E, Vidal, V, Marchesseau, S, Lagaude, A, Cuq, J-L & Tarodo de la Fuente, B 1998 Identification of interactions among casein gels using dissociating chemical agents. Journal of Dairy Science 81 932938CrossRefGoogle Scholar
Lucey, JA, Van Vliet, T, Grolle, K, Geurts, T & Walstra, P 1997a Properties of acid casein gels made by acidification with glucono-delta-lactone. I. Rheological properties. International Dairy Journal 7 381388CrossRefGoogle Scholar
Lucey, JA, Van Vliet, T, Grolle, K, Geurts, T & Walstra, P 1997b Properties of acid casein gels made by acidification with glucono-delta-lactone. II. Syneresis, permeability and microstructural properties. International Dairy Journal 7 389397CrossRefGoogle Scholar
Martens, R 1972 Influence de quelques facteurs sur la consistance et le goÛt du yoghourt brassé. Revue de L'Agriculture 3 461480Google Scholar
Martin, NC, Skokanova, J, Latrille, EFJ, Beal, CE & Corrieu, GV 1998 Sensory and instrumental characterization of the texture of stirred yoghurt. In: Texture of fermented milk products and dairy desserts proceedings of the IDF symposium held in Vicenza, Italy, 5–6 May 1997, pp. 2433. Brussels Belgium: International Dairy FederationGoogle Scholar
Martin, NC, Skokanova, J, Latrille, E, Beal, C & Corrieu, G 1999 Influence of fermentation and storage conditions on the sensory properties of plain low fat stirred yoghurts. Journal of Sensory Studies 14 139160CrossRefGoogle Scholar
Mekmene, O, Le Graet, Y & Gaucheron, F 2008 A model for predicting salt equilibria in milk and mineral-enriched milks. Food Chemistry submittedGoogle Scholar
Ozer, BH, Stenning, R, Grandison, A & Robinson, RK 1999 Effect of protein concentration on the properties and structure of concentrated yogurts. International Journal of Dairy Technology 52 135138CrossRefGoogle Scholar
Rasic, JLJ & Kurmann, JA 1978 Yoghurt: scientific grounds, technology, manufacture and preparations. Copenhagen, Denmark: The Technical Dairy Publishing HouseGoogle Scholar
Renan, M, Arnoult-Delest, V, Paquet, D, Brulé, G & Famelart, MH 2008a Changes in the rheological properties of stirred acid milk gels as induced by the acidification procedure. Dairy Science and Technology 88 341353CrossRefGoogle Scholar
Renan, M, Guyomarc'h, F, Arnoult-Delest, V, Pâquet, D, Brulé, G & Famelart, MH 2008b Rheological properties of stirred yoghurt as affected by gel pH at stirring, storage temperature and pH changes after stirring. International Dairy Journal submittedGoogle Scholar
Roefs, SPFM & Van Vliet, T 1990 Structure of acid casein gels. 2. Dynamic measurements and type of interaction forces. Colloids and Surfaces 50 161175CrossRefGoogle Scholar
Ronnegard, E & Dejmek, P 1993 Development and breakdown of structure in yoghurt studied by oscillatory rheological measurements. Le Lait 73 371379CrossRefGoogle Scholar
Schellhaass, SM & Morris, HA 1985 Rheological and scanning electron microscopic examination of skim milk gels obtained by fermenting with ropy and non-ropy strains of lactic acid bacteria. Food Microstructure 4 279287Google Scholar
Skriver, A, Roemer, H & Qvist, KB 1993 Rheological characterization of stirred yogurt: viscometry. Journal of Texture Studies 24 185198CrossRefGoogle Scholar
Sodini, I, Remeuf, F, Haddad, S & Corrieu, G 2004 The relative effect of milk base, starter, and process on yogurt texture: A review. Critical Review in Food Science and Nutrition 44 113137CrossRefGoogle ScholarPubMed
Van Marle, ME 1998 Structure and rheological properties of yoghurt gels and stirred yoghurts, PhD. pp. 1151. University of Twente, EnschedeGoogle Scholar
Van Marle, ME & Zoon, P 1995 Permeability and rheological properties of microbially and chemically acidified skim-milk gels. Netherlands Milk and Dairy Journal 49 4765Google Scholar
Vasbinder, AJ, Alting, AC, Visschers, RW & de Kruif, CG 2003 Texture of acid milk gels: formation of disulfide cross-links during acidification. International Dairy Journal 13 2938CrossRefGoogle Scholar