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Isolation and in vitro translation of mRNA from the calf abomasal mucosa and identification of an mRNA coding for a precursor of prochymosin

Published online by Cambridge University Press:  01 June 2009

Bruce H. Nicholson  and
Peter Jones
Bruce H. Nicholson
Affiliation:
Department of Physiology and Biochemistry, The University, Whiteknights, Reading RG6 2AJ, UK
Peter Jones
Affiliation:
Department of Physiology and Biochemistry, The University, Whiteknights, Reading RG6 2AJ, UK
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Summary

The mRNA coding for prochymosin (prorennin) has been partly purified from calf abomasum. The in vitro translation products of the total polyadenylated RNA show a major band on gel electrophoresis which reacts with antibody raised against purified chymosin. The mol. wt of 43000 is higher than expected from the reported amino acid sequence and would correspond to prochymosin with an unprocessed signal sequence of ∼ 17 amino acids. The synthesis of chymosin mRNA is age-related, and ceases by 3 months even in milk-fed calves.

Type
Original Articles
Information
Journal of Dairy Research , Volume 51 , Issue 1 , February 1984 , pp. 79 - 89
Copyright
Copyright © Proprietors of Journal of Dairy Research 1984

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