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Influence of microorganisms on retinol isomerization in milk

Published online by Cambridge University Press:  29 January 2008

Gianfranco Panfili ,
Alessandra Fratianni ,
Tiziana Di Criscio ,
Daniela Gammariello  and
Elena Sorrentino
Gianfranco Panfili*
Affiliation:
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy
Alessandra Fratianni
Affiliation:
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy
Tiziana Di Criscio
Affiliation:
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy
Daniela Gammariello
Affiliation:
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy
Elena Sorrentino
Affiliation:
DISTAAM, Università degli Studi del Molise, via De Sanctis, 86100 Campobasso, Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

In order to have a deeper insight into the retinol isomerization phenomenon, in this work different milk samples have been analysed for their content of trans retinol and its cis-isomers, by means of reliable HPLC techniques. Levels of the different isomers and the degree of retinol isomerization (13-cis/all-trans ratio, %) have been monitored during milk storage at different temperatures and after addition of specific microorganisms. In raw milk stored at 4°C for 96 h the degree of retinol isomerization shifted from 1·1 to 2·3%, while in raw milk stored at 22°C for 24 h it increased from 1·1 to 12·7%. Among microorganisms tested in pasteurized milk, the most active in causing an increment in the 13-cis/all-trans ratio (%), from 3·4 to 33·4% in 8 h, was Streptococcus thermophilus. The results obtained demonstrated a relationship between microbial evolution and retinol isomerization. Therefore, the determination of retinol isomers is of importance not only for a more precise evaluation of vitamin A activity but also for the evaluation of the microbiological quality of milk.

Keywords

Milkretinol isomerizationvitamin A
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 1 , February 2008 , pp. 37 - 43
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Brinkmann, E, Dehne, L, Oei, HB, Tiebach, R & Baltes, W 1995 Separation of geometrical retinol isomers in food samples by using narrow-bore high-performance liquid chromatography. Journal of Chromatography A 693 271–279CrossRefGoogle Scholar
Duarte-Favaro, RM, Iha, ME & Bianchi, MLP 2003 Liquid chromatographic determination of geometrical retinol isomers and carotene in enteral feeding formulas. Journal of Chromatography A 1021 125–132CrossRefGoogle Scholar
Erdman, JW, Poor, CL & Dietz, JM 1988 Factors affecting the bioavailability of vitamin A carotenoids and vitamin E. Food Technology 42 214–221Google Scholar
Gambelli, L, Manzi, P, Panfili, G, Vivanti, V & Pizzoferrato, L 1999 Constituents of nutritional relevance in fermented milk products commercialized in Italy. Food Chemistry 66 353–358CrossRefGoogle Scholar
Haroon, Y, Shearer, MJ, Rahim, S, Gunn, WG, Mcenery, G & Barkhan, P 1982 The content of phylloquinone (vitamin K1) in human milk, cows'milk and infant formula foods determined by high-performance liquid chromatography. Journal of Nutrition 112 1105–1117CrossRefGoogle ScholarPubMed
Holand, B, Unwin, ID & Buss, DH 1989 Milk products and eggs. In The Fourth supplement to McCance & Widdowson's. The composition of Foods, 4th ed (Eds Holand, B). Cambridge: The Royal Society of ChemistryGoogle Scholar
Jung, MY, Lee, KH & Kim, SY 1998 Retinyl palmitate isomers in skim milk during light storage as affected by ascorbic acid. Journal of Food Science 63 597–600CrossRefGoogle Scholar
Lampert, LM 1975 In Modern Dairy Products, 3rd ed (Ed. Lampert, LM). New York: Chemical PublishingGoogle Scholar
Le Marguer, I & Jackson, H 1983. Stability in vitamin A in pasteurized and ultra-high temperature processed milks. Journal of Dairy Science 66 2452–2458CrossRefGoogle Scholar
Marconi, E & Panfili, G 1998 Chemical composition and nutritional properties of commercial products of mare milk powder. Journal of Food Composition and Analysis 11 178–187CrossRefGoogle Scholar
Murphy, PA, Engelhardt, R & Smith, SE 1988 Isomerization of retinil palmitate in fortified skim milk under retail fluorescent light. Journal of Agricultural and Food Chemistry 36 592–595CrossRefGoogle Scholar
Panfili, G, Manzi, P & Pizzoferrato, L 1994 High performance liquid chromatographic method for the simultaneous determination of tocopherols, carotenes, and retinol and its geometric isomers in Italian cheeses. Analyst 119 1161–1165CrossRefGoogle ScholarPubMed
Panfili, G, Manzi, P & Pizzoferrato, L 1998 Influence of thermal and other manufacturing stresses on retinol isomerization in milk and dairy products. Journal of Dairy Research 65 253–260CrossRefGoogle ScholarPubMed
Panfili, G, Chimisso, E, Manzi, P & Pizzoferrato, L 1999 Il grado di isomerizzazione del retinolo può rappresentare un indice di processo in prodotti lattiero-caseari? Scienza e Tecnica Lattiero-Casearia 50 138–146Google Scholar
Perez, MD & Calvo, M 1995 Interaction of β-lactoglobulin with retinol and fatty acids and its role as a possible biological function for this protein: a review. Journal of Dairy Science 78 978–988CrossRefGoogle ScholarPubMed
Sierra, I, Prodanov, M, Calvo, M, Olano, A & Vidal-Valverde, C 1996 Vitamin stability and growth of psychrotrophic bacteria in refrigerated raw milk acidified with carbon dioxide. Journal of Food Protection 59 1305–1310CrossRefGoogle ScholarPubMed
Smith, DE & Berge, CB 1997 The effect of two antioxidants on the light stability of vitamin A in fluid milk. Milchwissenschaft-Milk Science International 52 551–554Google Scholar
Stancher, B & Zonta, F 1982 High-performance liquid chromatographic determination of carotene and vitamin A and its geometric isomers in foods. Application to cheese analysis. Journal of Chromatography 238 217–225CrossRefGoogle Scholar
Tateo, F 1978 In Analisi dei prodotti alimentari, pp. 33–34 (Ed. Tateo, ). Pinerolo, Italia: ChiriottiGoogle Scholar
Vassila, E, Badeka, A, Kondyli, E, Savvaidis, I & Kontominas, MG 2002 Chemical and microbiological changes in fluid milk as affected by packaging conditions. International Dairy Journal 12 715–722CrossRefGoogle Scholar
Weiser, H & Somorjai, G 1992 Bioactivity of cis and dicis isomers of vitamin A esters. International Journal for Vitamins and Nutrition Research 62 201–208Google ScholarPubMed
Woollard, DC & Fairweather, JP 1985 The storage stability of vitamin A in fortified ultra-high temperature processed milk. Journal of Micronutrient Analysis 1 13–21Google Scholar
Woollard, DC & Indyk, H 1986 The HPLC analysis of vitamin A isomers in dairy products and their significance in biopotency estimations. Journal of Micronutrient Analysis 2 125–146Google Scholar
Zahar, M, Smith, DE & Martin, F 1995 Vitamin A distribution among fat globule core, fat globule membrane, and serum fraction in milk. Journal of Dairy Science 78 498–505CrossRefGoogle ScholarPubMed