Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T03:38:18.599Z Has data issue: false hasContentIssue false
  • English
  • Français

Growth hormone gene variability and its effects on milk traits in primiparous Sarda goats

Published online by Cambridge University Press:  23 April 2013

Maria Luisa Dettori ,
Angela Maria Rocchigiani ,
Sebastiano Luridiana ,
Maria Consuelo Mura ,
Vincenzo Carcangiu ,
Michele Pazzola  and
Giuseppe Massimo Vacca
Maria Luisa Dettori*
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Angela Maria Rocchigiani
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Sebastiano Luridiana
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Maria Consuelo Mura
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Vincenzo Carcangiu
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Michele Pazzola
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
Giuseppe Massimo Vacca
Affiliation:
Dipartimento di Medicina Veterinaria, Università degli Studi di Sassari, via Vienna 2, 07100 Sassari, Italy
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of this research was to investigate variability in each of the five exons of the caprine growth hormone (gGH) gene, in order to establish the possible relationships with milk traits in Sarda breed goat. The general linear model procedure was used to analyse the effects of the single strand conformation (SSCP) profiles on milk traits of 100 lactating goats. Analysis of conformational polymorphism at exons 1–5 revealed a total of 25 differing banding patterns. Sequencing revealed 21 nucleotide changes (compared with GenBank D00476): 14 were polymorphic and 7 monomorphic; 19 in exonic regions, 5 of which were nonsynonymous. A SNP upstream of the transcription initiation codon (c.-3A>G) and an indel (c.*29_30insC) in the 3′UTR, were detected. Alignment of 4 cloned sequences including the entire gGH gene led to the identification of 22 nucleotide variations within the intron regions, including two indels. Association analysis revealed that each exon, except exon-1, affected milk yield, exons 1 and 3 influenced milk fat percentage, and all exons, except exon-2, had an effect on protein percentage, supporting previous results in livestock. The variability detected at the caprine GH gene might provide useful information for the phylogeny of ruminants and, more importantly, have implications on the biological function of the growth hormone and on those traits resulting from its physiological action, including milk production and composition. The caprine GH gene may become a useful molecular marker for a more effective genetic selection for milk production traits in goats.

Keywords

Goatgrowth hormonegGH genegenotypepolymorphism
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 3 , August 2013 , pp. 255 - 262
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baldi, A 1999 Manipulation of milk production and quality by use of somatotropin in dairy ruminants other than cow. Domestic Animal Endocrinology 17 131–137CrossRefGoogle ScholarPubMed
Barrera-Saldaña, HA, Ascacio-Martínez, JA, Sifuentes-Rincón, AM, Arellano-Vera, W & Arbiza, SI 2010 Applications of biotechnology and genomics in goats. Small Ruminant Research 89 81–90Google Scholar
Dettori, ML, Rocchigiani, AM, Pazzola, M, Carcangiu, V & Vacca, GM 2009 PCR-SSCP analysis of the GH gene in Sarda goats: a high variability and its preliminary effects on dairy performances. In: Proceedings of the 18th ASPA Congress, Palermo, Italy. Italian Journal of Animal Science 8 75–77Google Scholar
Falaki, M, Gengler, N & Sneyers, M 1996 Relationship of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. Journal of Dairy Science 79 1446–1453Google Scholar
Frankham, R, Ballou, JD & Briscoe, DA 2007 Genetic diversity. In: Introduction to Conservation Genetics, 1st edition. Cambridge University Press, New York, USA, pp. 47–50Google Scholar
Fries, R 1993 Mapping the bovine genome: methodological aspects and strategy. Animal Genetics 24 111–116CrossRefGoogle ScholarPubMed
Gupta, N, Ahlawat, SPS, Kumar, D, Gupta, SC, Pandey, A & Malik, G 2007 Single nucleotide polymorphism in growth hormone gene exon-4 and exon-5 using PCR-SSCP in Black Bengal goats – A prolific meat breed of India. Meat Science 76 658–665Google Scholar
Gupta, N, Pandey, A, Malik, G & Gupta, SC 2009 Single nucleotide polymorphism (SNP) in growth hormone gene of Jakhrana, a prominent milk goat breed in India. Small Ruminant Research 81 35–41CrossRefGoogle Scholar
Hall, TA 1999. BioEdit: A User-friendly Biological Sequence Alignment Editor and Analysis. http://www.mbio.ncsu.edu/BioEdit/bioedit.htmlGoogle Scholar
Hua, GH, Chen, SL, Yu, JN, Cai, KL, Wu, CJ, Li, QL, Zhang, CY, Liang, AX, Han, L, Geng, LY, Shen, Z, Xu, DQ & Yang, LG 2009 Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Science 81 391–395Google Scholar
Juárez-Aguilar, E, Castro-Muñozledo, F, Guerra-Rodríguez, NE, Reséndez-Pérez, D, Martínez-Rodríguez, HG, Barrera-Saldaña, HA & Kuri-Harcuch, W 1999 Functional domains of human growth hormone necessary for the adipogenic activity of hGH/hPL chimeric molecules. Journal of Cell Science 112 3127–3135CrossRefGoogle ScholarPubMed
Kioka, N, Manabe, E, Abe, M, Hashi, H, Yato, M, Okuno, M, Yamano, Y, Sakai, H, Komano, T, Utsumi, K & Iritani, A 1989 Cloning and sequencing of goat growth hormone gene. Agricultural and Biological Chemistry 53 1583–1587Google Scholar
Kumar, S, Dixit, SP, Gupta, SC, Vyas, MK & Kaur, J 2011 Genetic Variability of growth hormone gene and its association with growth traits in Sirohi breed of goat. Indian Journal of Animal Sciences 81 272–275Google Scholar
Lagziel, A, Lipkin, E, Ezra, E, Soller, M & Weller, JI 1999 An MspI polymorphism at the bovine growth hormone (bGH) gene is linked top a locus affecting milk protein percentage. Animal Genetics 30 296–299Google Scholar
Malveiro, E, Pereira, M, Marques, PX, Santos, IC, Belo, C, Renaville, R & Cravador, A 2001 Polymorphisms at the five exons of the growth hormone gene in the Algarvia goat: possible association with milk traits. Small Ruminant Research 41 163–170Google Scholar
Marques, PX, Pereira, M, Marques, MR, Santos, IC, Belo, C, Renaville, R & Cravador, A 2003 Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research 50 177–185Google Scholar
Marques, MR, Santos, IC, Carolino, N, Belo, C, Renaville, R & Cravador, A 2006 Effects of genetic polymorphisms at the growth hormone gene on milk yield in Serra da Estrela sheep. Journal of Dairy Research 73 394–405Google Scholar
Mattick, JS 2009 The genetic signatures of noncoding RNAs. PLoS Genetics 5 e1000459. doi:10.1371/journal.pgen.1000459Google Scholar
McDonald, MJ, Wang, W-C, Huang, H-D & Leu, J-Y 2011 Clusters of nucleotide substitutions and insertion/deletion mutations are associated with repeat sequences. PLoS Biology 9 e1000622. doi: 10.1371/journal.pbio.1000622Google Scholar
Ofir, R & Gootwine, E 1997 Ovine growth hormone gene duplication: structural and evolutionary implications. Mammalian Genome 8 770–772Google Scholar
Park, YW, Juarez, M, Ramos, M & Haenlein, GFW 2007 Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research 68 88–113Google Scholar
Pazzola, M, Dettori, M, Carcangiu, V, Luridiana, S, Mura, MC & Vacca, GM 2011 Relationship between milk urea, blood plasma urea and BCS in primiparous browsing goats with different milk yield level. ARCHIV FUR TIERZUCHT 54 546–556Google Scholar
Pazzola, M, Balia, F, Carcangiu, V, Dettori, M, Piras, G & Vacca, GM 2012 Higher somatic cell counted by the electronic counter method do not influence renneting properties of goat milk. Small Ruminant Research 102 32–36Google Scholar
Polkowska, J, Wańkowska, M, Romanowicz, K, Gajewska, A, Misztal, T & Wójcik-Gładysz, A 2011 The effect of intracerebroventricular infusions of ghrelin and/or short fasting on the gene expression and immunoreactivity of somatostatin in the hypothalamic neurons and on pituitary growth hormone in prepubertal female lambs. Morphological arguments. Brain Research 1414 41–49Google Scholar
Rubio, L, Ayllon, MA, Guerri, J, Pappu, H, Niblett, C & Moreno, P 1996 Differentiation of citrus tristeza closterovirus (CTV) isolates by single-strand conformation polymorphism analysis of the coat protein gene. Annals of Applied Biology 129 479–489Google Scholar
Scaramuzzi, RJ, Murray, JF, Downing, JA & Campbell, BK 1999 The effects of exogenous growth hormone on follicular steroid secretion and ovulation rate in sheep. Domestic Animal Endocrinology 17 269–277CrossRefGoogle ScholarPubMed
Tian, D, Wang, Q, Zhang, P, Araki, H, Yang, S, Kreitman, M, Nagylaki, T, Hudson, R, Bergelson, J & Chen, JQ 2008 Single-nucleotide mutation rate increases close to insertions/deletions in eukaryotes. Nature 455 105–108Google Scholar
Wallis, M, Lioupis, A & Wallis, OC 1998 Duplicate growth hormone genes in sheep and goat. Journal of Molecular Endocrinolpgy 21 1–5Google Scholar
Yeh, F, Yang, RC & Boyle, T 2000. Popgene V.1·32. Microsoft Windows based Freeware for Population Genetic Analysis. http://www.ualberta.ca/fyeh/index.htmGoogle Scholar
Zhang, C, Liu, Y, Huang, K, Zeng, W, Xu, D, Wen, Q & Yang, L 2011 The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds. Genetics and Molecular Biology 34 49–55Google Scholar
Zhou, GL, Liu, HG, Liu, C, Guo, SL, Zhu, Q & Wu, YH 2005 Association of genetic polymorphism in GH gene with milk production traits in Beijing Holstein cows. Journal of Biosciences 30 595–598Google Scholar