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Elevation of bovine serum C-reactive protein and serum amyloid P component levels by lactation

Published online by Cambridge University Press:  01 June 2009

Masami Morimatsu ,
Atsushi Watanabe ,
Kumiko Yoshimatsu ,
Toru Fujinaga ,
Masahiko Okubo  and
Masaharu Naiki
Masami Morimatsu
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Atsushi Watanabe
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Kumiko Yoshimatsu
Affiliation:
Department of Biochemistry, Hokkaido University, Sapporo 060, Japan
Toru Fujinaga
Affiliation:
Department of Surgery, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan
Masahiko Okubo
Affiliation:
Department of Animal Science, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan
Masaharu Naiki
Affiliation:
Department of Veterinary Science, National Institute of Health, Tokyo 141, Japan
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Summary

C-reactive protein (CRP) and serum amyloid P component (SAP), which are known to increase in sera from humans and many other animals with acute inflammation caused by infection, toxic drug administration or injury, were previously purified from bovine serum. These serum levels were determined by enzyme-linked immunosorbent assay (ELISA) using specific antiserum to bovine CRP or SAP which was prepared by immunizing rabbits and goats with each purified protein. Among 68 healthy Holstein cows, 45 non-lactating cows had levels of CRP and SAP of 20·6±1·4 and 27·6 ± 1·3 μg/ml respectively; 23 lactating cows had higher levels of CRP and SAP (76·0±13·6 and 38·3±5·5 μg/ml respectively). In the latter group, there was a high correlation between milk yield and serum CRP levels (P <0·001). From these observations, it was assumed that lactation might stimulate CRP synthesis rather than SAP synthesis in bovine liver as an acute phase reaction, and that CRP might be called a lactation-associated protein.

Type
Original Articles
Information
Journal of Dairy Research , Volume 58 , Issue 3 , August 1991 , pp. 257 - 261
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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References

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