Hostname: page-component-586b7cd67f-vdxz6 Total loading time: 0 Render date: 2024-11-30T19:07:44.092Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of insulin in conjunction with glucose, amino acids and potassium on net metabolism of glucose and amino acids in the goat mammary gland

Published online by Cambridge University Press:  01 June 2009

Sophie Tesseraud ,
Jean Grizard ,
Boguslaw Makarski ,
Elisabeth Debras ,
Gerard Bayle  and
Claude Champredon
Sophie Tesseraud
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Jean Grizard
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Boguslaw Makarski
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Elisabeth Debras
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Gerard Bayle
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Claude Champredon
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Get access Rights & Permissions [Opens in a new window]

Summary

The hyperinsulinaemic euglycaemic insulin clamp technique was used to study the effect of insulin on the arterio-venous concentration differences of glucose and amino acids across the mammary gland in dairy goats. Insulin was given in conjunction with K to prevent insulin hypokalaemia. Appropriate amino acid infusion was used to blunt insulin-induced hypoaminoacidaemia or to create hyperaminoacidaemia and maintain this state under insulin treatment. Hyper-aminoacidaemia alone only stimulated mammary leucine uptake but did not significantly modify the net metabolism of other amino acids and glucose. Insulin infusion at physiological level in conjunction with glucose, KCl-NaCl and amino acids failed to alter mammary uptake of glucose and essential amino acids; occasional increase in arginine extraction and decrease in tyrosine extraction were exceptions. Thus these new experimental conditions did not reveal any galactopoietic effect of insulin.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 2 , May 1992 , pp. 135 - 149
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Annison, E. F. 1983 Metabolite utilization by the ruminant mammary gland. In Biochemistry of Lactation pp. 399–436 (Ed. Mepham, T. B.). Amsterdam: Elsevier Science PublishersGoogle Scholar
Bauman, D. E. & Elliot, J. M. 1983 Control of nutrient partitioning in lactating ruminants. In Biochemistry of Lactation pp. 437–468 (Ed. Mepham, T. B.). Amsterdam: Elsevier Science Publishers.Google Scholar
Baumrucker, C. R. & Stemberger, B. H. 1989 Insulin and insulin-like growth factor-I stimulate DNA synthesis in bovine mammary tissue in vitro. Journal of Animal Science 67 3505–3514CrossRefGoogle ScholarPubMed
Bennet, W. M., Connacher, A. A., Scrimgeour, C. M., Jung, R. T. & Rennie, M. J. 1990 Euglycemic hyperinsulinemia augments amino acid uptake by human leg tissues during hyperaminoacidemia. American Journal of Physiology 259 E185–E194Google Scholar
Bennet, W. M., Connacher, A. A., Scrimgeour, C. M., Smith, K. & Rennie, M. J. 1989 Increase in anterior tibialis muscle protein synthesis in healthy man during mixed amino acid infusion: studies of incorporation of [1-13C] leucine. Clinical Science 76 447–454Google Scholar
Bloxam, D. L. & Warren, W. H. 1974 Error in the determination of tryptophan by the method of Denkla and Dewey. A revised procedure. Analytical Biochemistry 60 621–625Google Scholar
Brockman, R. P., Bergman, E. N., Joo, P. K. & Manns, J. G. 1975 Effects of glucagon and insulin on net hepatic metabolism of glucose precursors in sheep. American Journal of Physiology 229 1344–1350Google Scholar
Burnol, A. P., Ebner, S., Ferré, P. & Girard, J. 1988 Regulation by insulin of glucose metabolism in mammary gland of anaesthetized lactating rats. Stimulation of phosphofructokinase-1 by fructose 2,6-biphosphate and activation of acetyl-CoA carboxylase. Biochemical Journal 254, 11–14CrossRefGoogle ScholarPubMed
Burnol, A. F., Ferré, P., Leturque, A. & Girard, J. 1987 Effect of insulin on in vivo glucose utilization in individual tissues of anesthetized lactating rats. American Journal of Physiology 252 E183–E188Google Scholar
Burnol, A. F., Leturque, A., Ferré, P. & Girard, J. 1983 Glucose metabolism during lactation in the rat: quantitative and regulatory aspects. American Journal of Physiology 245 E351–E358Google Scholar
Campbell, P. G., Frey, D. M. & Baumrucker, C. R. 1987 Changes in bovine mammary insulin binding during pregnancy and lactation. Comparative Biochemistry and Physiology 87B 649–653Google Scholar
Castellino, P., Luzi, L., Simonson, D. C., Haymond, M. & De Fronzo, R. A. 1987 Effect of insulin and plasma amino acid concentrations on leucine metabolism in man. Role of substrate availability on estimates of whole body protein synthesis. Journal of Clinical Investigation 80 1784–1793Google Scholar
Chaiyabutr, N., Faulkner, A. & Peaker, M. 1983 Effects of exogenous glucose on glucose metabolism in the lactating goat in vivo. British Journal of Nutrition 49 159–165CrossRefGoogle ScholarPubMed
Champredon, C., Pion, R. & Prugnaud, J. 1977 [Effect of intravenous methionine infusion on amino acid levels in dairy goats.] Annales de Biologie Animale, Biochimie, Biophysique 17 85–93Google Scholar
Davis, S. R. & Bickerstaffe, R. 1978 Mammary glucose uptake in the lactating ewe and the use of methionine arterio-venous difference for the calculation of mammary blood flow. Australian Journal of Biological Science 31 133–139Google Scholar
Davis, S. R., Bickerstaffe, R. & Hart, D. S. 1978 Amino acid uptake by the mammary gland of the lactating ewe. Australian Journal of Biological Science 31 123–132Google Scholar
Debras, E., Grizard, J., Aina, E., Tesseraud, S., Champredon, C. & Arnal, M. 1989 Insulin sensitivity and responsiveness during lactation and dry period in goats. American Journal of Physiology 256 E295–E302Google Scholar
Early, R. J., McBride, B. W. & Ball, R. O. 1988 a Phenylalanine metabolism in sheep infused with glucose plus insulin. I. Effects on plasma phenylalanine concentration, entry rate and utilization by the hindlimb. Canadian Journal of Animal Science 68 711–719Google Scholar
Early, R. J., McBride, B. W. & Ball, R. O. 1988 b Phenylalanine metabolism in sheep infused with glucose plus insulin. II. Effects on in vivo and in vitro protein synthesis and related energy expenditures. Canadian Journal of Animal Science 68 721–730Google Scholar
Flakoll, P. J., Kulaylat, M., Frexes-Steed, M., Hourani, H., Brown, L. L., Hill, J. O. & Abumrad, N. N. 1989 Amino acids augment insulin's suppression of whole body proteolysis. American Journal of Physiology 257, E839–E847Google Scholar
Fleet, I. R. & Mepham, T. B. 1985 Mammary uptake of amino acids and glucose throughout lactation in Friesland sheep. Journal of Dairy Research 52 229–237Google Scholar
Fukagawa, N. K., Minaker, K. L., Rowe, J. W., Goodman, M. N., Matthews, D. E., Bier, D. M. & Young, V. R. 1985 Insulin-mediated reduction of whole body protein breakdown. Dose-response effects on leucine metabolism in postabsorptive men. Journal of Clinical Investigation 76 2306–2311CrossRefGoogle ScholarPubMed
Fukagawa, N. K., Minaker, K. L., Young, V. R., Matthews, D. E., Bier, D. M. & Rowe, J. W. 1989 Leucine metabolism in aging humans: effect of insulin and substrate availability. American Journal of Physiology 256 E288–E294Google Scholar
Garlick, P. J. & Grant, I. 1988 Amino acid infusion increases the sensitivity of muscle protein synthesis in vivo to insulin. Effect of branched-chain amino acids. Biochemical Journal 254 579–584CrossRefGoogle ScholarPubMed
Hove, K. 1978 Effects of hyperinsulinaemia on lactose secretion and glucose uptake by the goat mammary gland. Acta Physiologica Scandinavica 104 422–430Google Scholar
Jones, R. G., Ilic, V. & Williamson, D. H. 1984 Regulation of lactating-rat mammary-gland lipogenesis by insulin and glucagon in vivo. The role and site of action of insulin in the transition to the starved state. Biochemical Journal 223 345–351Google Scholar
Laarveld, B., Christensen, D. A. & Brockman, R. P. 1981 The effect of insulin on net metabolism of glucose and amino acids by the bovine mammary gland. Endocrinology 108 2217–2221Google Scholar
Linzell, J. L. 1967 The effect of infusions of glucose, acetate and amino acids on hourly milk yield in fed, fasted and insulin-treated goats. Journal of Physiology 190 347–357Google Scholar
Linzell, J. L. & Mepham, T. B. 1974 Effects of intramammary arterial infusion of essential amino acids in the lactating goat. Journal of Dairy Research 41 101–109CrossRefGoogle Scholar
Mepham, T. B. 1988 Nutrient uptake by the lactating mammary gland. In Nutrition and Lactation in the Dairy Cow pp. 15–31 (Ed. Garnsworthy, P. C.). London: ButterworthsGoogle Scholar
Mepham, T. B. & Linzell, J. L. 1966 A quantitative assessment of the contribution of individual plasma amino acids to the synthesis of milk proteins by the goat mammary gland. Biochemical Journal 101 76–83CrossRefGoogle Scholar
Mepham, T. B. & Linzell, J. L. 1974 Hour-to-hour variation in amino acid arterio-venous concentration differences across the lactating goat mammary gland. Journal of Dairy Research 41 95–100CrossRefGoogle ScholarPubMed
Nair, K. S., Ford, G. C. & Halliday, D. 1987 Effect of intravenous insulin treatment on in vivo whole body leucine kinetics and oxygen consumption in insulin-deprived type I diabetic patients. Metabolism 36 491–495Google Scholar
Oddy, V. H., Lindsay, D. B., Barker, P. J. & Northrop, A. J. 1987 Effect of insulin on hind-limb and whole-body leucine and protein metabolism in fed and fasted lambs. British Journal of Nutrition 58 437–452Google Scholar
Oldham, J. D. 1981 Amino acid requirements for lactation in high-yielding dairy cows. In Recent Advances in Animal Nutrition - 1980 pp. 33–65 (Ed. Haresign, W.). London: ButterworthsCrossRefGoogle Scholar
Park, C. S., Smith, J. J., Eigel, W. N. & Keenen, T. W. 1979 Selected hormonal effects on protein secretion and amino acid uptake by acini from bovine mammary gland. International Journal of Biochemistry 10 889–894Google Scholar
Peeters, G., Houvenaghel, A., Roets, E., Massart-Leen, A. M., Verbeke, R., Dhondt, G. & Verschooten, F. 1979 Electromagnetic blood flow recording and balance of nutrients in the udder of lactating cows. Journal of Animal Science 48 1143–1153Google Scholar
Reynolds, M., Linzell, J. L. & Rasmussen, F. 1968 Comparison of four methods for measuring mammary blood flow in conscious goats. American Journal of Physiology 214 1415–1424Google Scholar
Shamay, A., Cohen, N., Niwa, M. & Gertler, A. 1988 Effect of insulin-like growth factor I on deoxyribonucleic acid synthesis and galactopoiesis in bovine undifferentiated and lactating mammary tissue in vitro. Endocrinology 123 804–809CrossRefGoogle ScholarPubMed
Tessari, P., Inchiostro, S., Biolo, G., Trevisan, R., Fantin, G., Marescotti, M. C., Iori, E., Tiengo, A. & Crepaldi, G. 1987 Differential effects of hyperinsulinemia and hyperaminoacidemia on leucine-carbon metabolism in vivo. Evidence for distinct mechanisms in regulation of net amino acid deposition. Journal of Clinical Investigation 79 1062–1069CrossRefGoogle ScholarPubMed
Topper, Y. J., Nicholas, K. R., Sankaran, L. & Kulski, J. 1984 Insulin as a developmental hormone. In Hormones and Cancer pp 63–77 (Eds Gurpide, E., Calandra, R., Levy, C. and Soto, R. J.). New York: LissGoogle Scholar
Vernon, R. G. 1989 Endocrine control of metabolic adaptation during lactation. Proceedings of the Nutrition Society 48 23–32CrossRefGoogle ScholarPubMed
Weekes, T. E. C. 1986 Insulin and growth. In Control and Manipulation of Animal Growth pp. 187–222 (Eds Buttery, P. J., Lindsay, D. B. and Hayne, N. B.). London: ButterworthsCrossRefGoogle Scholar
Williamson, D. H. 1986 Regulation of metabolism during lactation in the rat. Reproduction, Nutrition, Développements 26 597–603Google Scholar
Yalow, R. S. & Berson, S. A. 1960 Immunoassay of endogenous plasma insulin in man. Journal of Clinical Investigation 39 1157–1175CrossRefGoogle ScholarPubMed