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Discrete phosphorylation generates the electrophoretic heterogeneity of ovine β-casein

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Giuseppina Garro ,
Pasquale Ferranti ,
Antonio Malorni ,
Francesco Addeo ,
Araceli Rabasco  and
Pilar Molina Pons
Lina Chianese
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Giuseppina Garro
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Pasquale Ferranti
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Antonio Malorni
Affiliation:
Servizio di Spettrometria di Massa, CNR, Via Pansini 5, 1-80131 Napoli, Istituto per la Chimica delle Molecole di Interesse Biologic, CNR, 1-80072 Arcofeli, Italia
Francesco Addeo
Affiliation:
Dipartimento di Scienza degli Alimenti, Facoltà di Agraria, Università degli Studi di Napoli Federico II, Parco Gussone, 1-80055 Portici, Italia
Araceli Rabasco
Affiliation:
Departamento Genetica, Facultad de Veterinar, Universidad de Estremadu, E-10071 Caceres, España
Pilar Molina Pons
Affiliation:
Departamento de Ciencia Animal, Universidad Politecnica de Valencia, E-46071 Valencia, España
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Summary

The heterogeneity of ovine β-casein, apparent after discontinuous gel electrophoresis at alkaline pH and gel isoelectric focusing either by staining with Coomassie blue or immunoblotting, was investigated in detail. Electrospray mass spectrometry was used to characterize ovine casein by direct examination of whole casein samples. β-Casein in individual milks consisted of several components which were characterized through the accurate determination of their molecular masses. It was demonstrated that protein species occurring simultaneously in individual ovine milks differed mainly in the number of phosphate groups.

Type
Original articles
Information
Journal of Dairy Research , Volume 62 , Issue 1 , February 1995 , pp. 89 - 100
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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