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Antimicrobial susceptibility and biofilm forming ability of staphylococci from subclinical buffalo mastitis

Published online by Cambridge University Press:  13 March 2023

Ece Koldaş Ürer ,
Özkan Aslantaş ,
Erhan Tek ,
Mehmet Ali Yılmaz  and
Yaşar Ergün
Ece Koldaş Ürer
Affiliation:
Department of Obstetrics and Gynecology, Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, 31060, Hatay, Turkey
Özkan Aslantaş*
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060, Hatay, Turkey
Erhan Tek
Affiliation:
Department of Microbiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060, Hatay, Turkey
Mehmet Ali Yılmaz
Affiliation:
International Center for Livestock Research and Training, Lalahan, Ankara, Turkey
Yaşar Ergün
Affiliation:
Department of Obstetrics and Gynecology, Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, 31060, Hatay, Turkey
*
Author for correspondence: Özkan Aslantaş, Email: ozkanaslantas@yahoo.com/aslantas@mku.edu.tr
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Abstract

The starting objective of this research communication was to determine the prevalence of subclinical mastitis in buffalo in Turkey. We also seeked to isolate and identify staphylococci, determine their antimicrobial susceptibilities and biofilm-forming abilities as well as investigating the presence of biofilm-related genes and microbial surface components recognizing adhesive matrix molecules. A total of 107 (66.9%) staphylococci (28 S. aureus and 79 coagulase-negative staphylococci, CoNS) were isolated from 160 mastitic milk samples collected from 200 lactating water buffalos. The staphylococci were especially resistant to beta-lactams except for cefoxitin but were less resistant to the other antimicrobials that were tested. Based on the Congo red agar method, 92.9% of the S. aureus and 70.9% of the CoNS isolates were positive for biofilm-forming ability, while all S. aureus and 97.5% of CoNS isolates were positive by a microtiter plate analysis. The presence of icaA and icaD genes was not always correlated with biofilm synthesis, and even in the absence of these genes, the isolates were able to synthesize biofilm.

Keywords

Antibiotic resistancebiofilmbuffalomastitisstaphylococci
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 1 , February 2023 , pp. 38 - 41
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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References

Ciftci, A, Findik, A, Onuk, EE and Savasan, S (2009) Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis. Brazilian Journal of Microbiology 40, 254–261.CrossRefGoogle ScholarPubMed
Clinical and Laboratory Standards Institute (CLSI) (2022) Performance standards for antimicrobial susceptibility testing. CLSI document: M100-32. Wayne, PA, USA.Google Scholar
Cramton, SE, Gerke, C, Schnell, NF, Nichols, WW and Götz, F (1999) The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infection and Immunity 67, 5427–5433.CrossRefGoogle ScholarPubMed
Darwish, SF and Asfour, HA (2013) Investigation of biofilm forming ability in Staphylococci causing bovine mastitis using phenotypic and genotypic assays. The Scientific World Journal 2013, 1–9.CrossRefGoogle ScholarPubMed
de Souza Santos, A, de Lima, DCV, Abad, ACA, de Oliveira, PRF, da Silva, JG, de Moura, GS and Mota, RA (2020) Antimicrobial resistance profile of non-aureus Staphylococci isolates from buffalo, goat and sheep mastitis in the Northeast region of Brazil. Journal of Dairy Research 87, 290–294.CrossRefGoogle Scholar
Elhaig, MM and Selim, A (2015) Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt. Tropical Animal Health and Production 47, 271–276.CrossRefGoogle ScholarPubMed
Felipe, V, Morgante, CA, Somale, PS, Varroni, F, Zingaretti, ML, Bachetti, RA, Correa, SG and Porporatto, C (2017) Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms. Microbial Pathogenesis 104, 278–286.CrossRefGoogle ScholarPubMed
Foster, TJ and Höök, M (1998) Surface protein adhesins of Staphylococcus aureus. Trends in Microbiology 12, 484–488.CrossRefGoogle Scholar
Gomes, F, Saavedra, MJ and Henriques, M (2016) Bovine mastitis disease/pathogenicity: evidence of the potential role of microbial biofilms. FEMS Pathogens and Disease 74(3), ftw006.CrossRefGoogle ScholarPubMed
Liberto, M, Mater, G, Quirino, A, Lamberti, AG, Capicotto, R, Puccio, R, Barreca, GS, Focà, E, Cascio, A and Focà, A (2009) Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques. Microbiological Research 164, 522–528.CrossRefGoogle ScholarPubMed
Medeiros, ES, França, CA, Krewer Cda, C, Peixoto Rde, M, de Souza, AF, Cavalcante, MB Jr, da Costa, MM and Mota, RA (2011) Antimicrobial resistance of Staphylococcus spp. isolates from cases of mastitis in buffalo in Brazil. Journal of Veterinary Diagnostic Investigation 23, 793–796.CrossRefGoogle ScholarPubMed
Melchior, MB, van Osch, MHJ, Lam, TJGM, Vernooij, JCM, Gaastra, W and Fink-Gremmels, J (2011) Extended biofilm susceptibility assay for Staphylococcus aureus bovine mastitis isolates: evidence for association between genetic makeup and biofilm susceptibility. Journal of Dairy Science 94, 5926–5937.CrossRefGoogle ScholarPubMed
Oliver, SP and Murinda, SE (2012) Antimicrobial resistance of mastitis pathogens. Review. Veterinary Clinics of North America: Food Animal Practice 28, 165–185.Google Scholar
Özenç, E, Vural, MR, Şeker, E and Uçar, M (2008) An evaluation of subclinical mastitis during lactation in Anatolian buffaloes. Turkish Journal of Veterinary and Animal Sciences 32, 359–368.Google Scholar
Preethirani, PL, Isloor, S, Sundareshan, S, Nuthanalakshmi, V, Deepthikiran, K, Sinha, AY, Rathnamma, D, Nithin Prabhu, K, Sharada, R, Mukkur, TK and Hegde, NR (2015) Isolation, biochemical and molecular identification, and in-vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India. PLoS ONE 10, e0142717.CrossRefGoogle ScholarPubMed
Quinn, PJ, Carter, ME, Markey, BK and Carter, GR (1999) Clinical Veterinary Microbiology. Mosby, Edinburgh, UK.Google Scholar
Sharif, A and Ahmad, T (2007) Prevalence of severity of mastitis in buffaloes in district Faisalabad (Pakistan). Journal of Agriculture and Social Science 3, 34–36.Google Scholar
Singha, S, Ericsson, CD, Chowdhury, S, Nath, SC, Paul, OB, Hoque, MA and Rahman, MM (2021) Occurrence and aetiology of subclinical mastitis in water buffalo in Bangladesh. Journal of Dairy Research 88, 314–320.CrossRefGoogle ScholarPubMed
Tremblay, YD, Lamarche, D, Chever, P, Haine, D, Messier, S and Jacques, M (2013) Characterization of the ability of coagulase-negative staphylococci isolated from the milk of Canadian farms to form biofilms. Journal of Dairy Science 96, 234–246.CrossRefGoogle ScholarPubMed
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