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The 31P nuclear magnetic resonance spectrum of cows' milk

Published online by Cambridge University Press:  01 June 2009

Peter S. Belton ,
Richard L. J. Lyster  and
Colin P. Richards
Peter S. Belton
Affiliation:
Food Research Institute, Norwich NR4 7UA, UK
Richard L. J. Lyster
Affiliation:
† ‡National Institute for Research in Dairying (University of Reading), Shinfield, Reading RG2 9 AT, UK
Colin P. Richards
Affiliation:
§Laboratory of the Government Chemist, London SE1 9NQ, UK
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Summary

The 31P nuclear magnetic resonance spectrum of liquid milk was examined. Of the three peaks observed, the two larger were assigned to inorganic phosphate (Pi) and the seryl phosphate (SerP) residues of casein; the third peak was assigned to a phosphodiester, which is probably glycerophosphoryl choline. The pH-dependences of the chemical shifts of the Pi and SerP were measured with and without added EDTA, and the results confirm the assignments. The width of the Pi peak in milk is significantly greater than in similar solutions lacking casein, probably because of binding to, or chemical exchange with, the casein micelle. Most of the SerP residues in milk are not sufficiently mobile to have been detected in these experiments but a significant fraction of SerP residues is able to move freely and can be titrated.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 1 , February 1985 , pp. 47 - 54
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

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