Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-04T22:43:34.711Z Has data issue: false hasContentIssue false
  • English
  • Français

381. A rapid and simple phosphatase test for milk

Published online by Cambridge University Press:  01 June 2009

R. Aschaffenburg  and
J. E. C. Mullen
R. Aschaffenburg
Affiliation:
National Institute for Research in Dairying, University of Reading
J. E. C. Mullen
Affiliation:
National Institute for Research in Dairying, University of Reading
Get access Rights & Permissions [Opens in a new window]

Extract

A simple and rapid phosphatase test is described which is based on the use of p–nitrophenylphosphate as the substrate. Execution of the test requires few and easy operations: incubation of the milk at 37° C. with a buffered solution of the substrate, followed directly by a visual comparison of the intensity of yellow colour of liberated p–nitrophenol with that of boiled-milk standards containing known amounts of p–nitrophenol. The test permits the detection of minor processing faults with an incubation time of only 30 min. In this form it should prove of value in creamery control. A more searching, but equally simple test is obtained by increasing the period of incubation to 2 hr.

Type
Original Articles
Information
Journal of Dairy Research , Volume 16 , Issue 1 , January 1949 , pp. 58 - 67
Copyright
Copyright © Proprietors of Journal of Dairy Research 1949

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

(1)Kay, H. D. & Graham, W. R. Jnr (1935). J. Dairy Res. 6, 191.Google Scholar
(2)Aschaffenburg, R. & Neave, F. K. (1939). J. Dairy Res. 10, 485.CrossRefGoogle Scholar
(3)Scharer, H. (1938). J. Dairy Sci. 21, 21; J. Milk Tech. 1 (5), 35.Google Scholar
(4)Huggins, C. & Talalay, P. (1945). J. biol. Chem. 159, 399.Google Scholar
(5)Bessey, O. A., Lowry, O. H. & Brock, M. J. (1946). J. biol. Chem. 164, 321.CrossRefGoogle Scholar
(6)Stiven, D. (1947). J. Dairy Res. 15, 57.CrossRefGoogle Scholar
(7)Hartmann, S. & Andersen, K. (1942–1943). Beretn. Forsoksm., Kbh., No. 38, p. 15.Google Scholar
(8)Ohmori, Y. (1937). Enzymologia, 4, 217.Google Scholar
(9)Axelrod, B. (1947). J. biol. Chem. 167, 57.CrossRefGoogle Scholar
(10)King, E. J. & Delory, G. E. (1940). Enzymologia, 8, 278.Google Scholar
(11)Horwitz, W. (1946). J. Ass. off. agric. Chem., Wash., 29, 129.Google Scholar