Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-24T03:37:58.699Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro inhibition studies of natural resin acids to Clostridium perfringens, Staphylococcus aureus and Escherichia coli O149

Published online by Cambridge University Press:  06 March 2018

Krisna Roy ,
Ulrike Lyhs ,
Juhani Vuorenmaa  and
Karl Pedersen
Krisna Roy*
Affiliation:
Technical University of Denmark, National Veterinary Institute, Kemitorvet, DK-2800 Kongens Lyngby, Denmark. Department of Pathology and Parasitology, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Chittagong-4225, Bangladesh.
Ulrike Lyhs
Affiliation:
Technical University of Denmark, National Veterinary Institute, Kemitorvet, DK-2800 Kongens Lyngby, Denmark.
Juhani Vuorenmaa
Affiliation:
Research and Development, Hankkija Oy / Suomen Rehu, 05801 Hyvinkaa, Finland.
Karl Pedersen
Affiliation:
Technical University of Denmark, National Veterinary Institute, Kemitorvet, DK-2800 Kongens Lyngby, Denmark.
*
*Corresponding author Tel.: +88 -01796299974, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The following experiment evaluated the inhibitory activity of a resin acids-based product (RAP) to bacterial pathogens. Clostridium perfringens isolated from chickens, turkeys and pigs, Staphylococcus aureus from chickens, pigs and cattle, and Escherichia coli O149 isolated from pigs were tested. Two different methods were used, a broth dilution method (BDM) using 0.01%, 0.1% and 0.5% resin acid, and an agar diffusion method (ADM) using 0.01%, 0.1%, 0.5%, 1% and 5% resin acid. For the BDM, C. perfringens was inhibited completely at all concentrations. S. aureus was inhibited completely at 0.5%, but only slightly at 0.1% and not at all at 0.01%. The E. coli strains showed no or little inhibition at 0.5%. For the ADM, narrow inhibition zones evolved around the concentration of 0.5% (8–10 mm), 1% (8.0–12.0 mm), and 5% (9.0–19.5 mm) on the C. perfringens strains, while the inhibition zones for S. aureus were smaller and E. coli developed no inhibition zones. Overall, the RAP inhibited C. perfringens at all concentrations of the product, S. aureus at 0.1%, 0.5%, 1% and 5% concentrations, and E. coli O149 only at 0.5% concentrations, although some strain variation was recorded.

Keywords

In vitroinhibitionbacteriaresin-acids
Type
Original Research
Information
Journal of Applied Animal Nutrition , Volume 6 , 2018 , e2
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bartolo, J. and Sall, K. (2015) Tannin based feed additives as a natural way in reducing incidence of necrotic enteritis. Abstract. 1st International Conference on Necrotic Enteritis in Poultry, Copenhagen, Denmark, pp. 35.Google Scholar
Cariou, N., Fadel, C. and Rothstein, T. (2015) Activo® Liquid used for prevention of necrotic enteritis on free range label broilers vaccinated with Paracox®−5. Abstract. 1st International Conference on Necrotic Enteritis in Poultry, Copenhagen, Denmark, pp. 56.Google Scholar
Duijkeren, E.V., Ikawaty, R., Broekhuizen-Stins, M.J., Jansen, M.D., Spalburg, E.C., de Neeling, A.J., Allaart, J.G., Van Nes, A., Wagenaar, J.A. and Fluit, A.C. (2008) Transmission of methicillin-resistant Staphylococcus aureus strains between different kinds of pig farms. Veterinary Microbiology, 126: 383–389.Google Scholar
Fairbrother, J.M. and Gyles, C.L. (2006) Escherichia coli infections, in: Straw, B.E., Zimmerman, J.J., D'Allaire, S. & Taylor, D.J (Eds) Diseases in swine, 9th edn pp. 639–674. (UK, Oxford, Blackwell Publishing).Google Scholar
Huyghebaert, G., Ducatelle, R. and Van Immerseel, F. (2011) An update on alternatives to antimicrobial growth promoters for broilers. Veterinary Journal, 187: 182–188.Google Scholar
Jokinen, J.J. and Sipponen, A. (2016) Refined spruce resin to treat chronic wounds: Rebirth of an old folkloristic therapy. Advances in Wound Care, 5: 198–207.Google Scholar
Kettunen, H., Vuorenmaa, J., Rinttila, T., Gronberg, H., Valkonen, E. and Apajalahti, J. (2015) Natural resin acid enriched composition as a modulator of intestinal microbiota and performance enhancer in broiler chicken. Journal of Applied Animal Nutrition, 3: 1–9.CrossRefGoogle Scholar
Kettunen, H., Eerden, E. V., Lipinski, K., Rinttila, T., Valkonen, E. and Vuorenmaa, J. (2017) Dietary resin acid composition as a performance enhancer for broiler chickens. Journal of Applied Animal Nutrition, 5: 1–8.Google Scholar
Lyhs, U., Perko-Makela, P., Kallio, H., Brockmann, A., Heinikainen, S., Tuuri, H. and Pedersen, K. (2013) Characterization of Clostridium perfringens isolates from healthy turkeys and from turkeys with necrotic enteritis. Poultry Science, 92: 1750–1757.CrossRefGoogle ScholarPubMed
Nagase, N., Sasaki, A., Yamashita, K., Shimizu, A., Wakita, Y., Kitai, S. and Kawa, J. (2002) Isolation and species distribution of staphylococci from animal and human Skin. Journal of Veterinary Medical Science, 64: 245–250.Google Scholar
Nauerby, B., Pedersen, K. and Madsen, M. (2003) Analysis by pulsed-field gel electrophoresis of the genetic diversity among Clostridium perfringens isolates from chickens. Veterinary Microbiology, 94: 257–266.CrossRefGoogle ScholarPubMed
Noamani, B.N., Fairbrother, J.M. and Gyles, C.L. (2003) Escherichia coli from outbreaks of post-weaning diarrhea in pigs. Veterinary Microbiology, 97: 87–101.Google Scholar
Rubio, J., Calderón, J.S., Flores, A., Castroa, C. and Céspedes, C.L. (2005) Trypanocidal activity of oleoresin and terpenoids isolated from Pinus oocarpa . Zeitschrift für Naturforschung, 60: 711–716.Google Scholar
San Feliciano, A., Gordaliza, M., Salinero, M.A. and Miguel del Corral, J.M. (1993) Abietane acids; Sources, biological activities, and therapeutic uses. Planta Medica, 59: 485–490.CrossRefGoogle ScholarPubMed
Savluchinske-Feio, S., Curto, M.J., Gigante, B. and Roseiro, J.C. (2006) Antimicrobial activity of resin acid derivatives. Applied Microbiology and Biotechnology, 72: 430–436.Google Scholar
Savluchinske-Feio, S., Gigante, B., Roseiro, J.C. and Marcelo-Curto, M.J. (1999) Antimicrobial activity of diterpene resin acid derivatives. Journal of Microbiological Methods, 35: 201–206.CrossRefGoogle ScholarPubMed
Smith, E., Williamson, E., Zloh, M. and Gibbons, S. (2005) Isopimaric acid from Pinus nigra shows activity against multidrug-resistant and EMRSA strains of Staphylococcus aureus . Phytotherapy Research, 19: 538–542.Google Scholar
Söderberg, T.A., Johansson, A. and Gref, R. (1996) Toxic effects of some conifer resin acids and tea tree oil on human epithelial and fibroblast cells. Toxicology, 107: 99–109.Google Scholar
Songer, J.G. (1996) Clostridial enteric diseases of domestic animals. Clinical Microbiology, 9: 216–234.Google Scholar
Tellez, G., Latorre, J.D. and Hargis, B.M. (2015) Efficacy of lactic acid base probiotics or Bacillus direct-fed microbials (DFM) candidates as alternatives to antibiotics to control necrotic enteritis in poultry. Abstract. 1st International Conference on Necrotic Enteritis in Poultry, Copenhagen, Denmark, pp. 41.Google Scholar
Timbermont, L., Haesebrouck, F., Ducatelle, R. and Van Immerseel, F. (2011) Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian Pathology, 40: 341–317.CrossRefGoogle ScholarPubMed