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Production of consistent crush lesions of murine skeletal muscle in vivo using an electromechanical device

Published online by Cambridge University Press:  01 April 1997

J. L. RUSHTON ,
I. DAVIES ,
M. A. HORAN ,
M. MAHON  and
R. WILLIAMS
J. L. RUSHTON
Affiliation:
School of Biological Sciences, University of Manchester, UK Department of Geriatric Medicine, University Hospital of South Manchester, Manchester, UK
I. DAVIES
Affiliation:
School of Biological Sciences, University of Manchester, UK Department of Geriatric Medicine, University Hospital of South Manchester, Manchester, UK
M. A. HORAN
Affiliation:
Department of Geriatric Medicine, University Hospital of South Manchester, Manchester, UK
M. MAHON
Affiliation:
School of Biological Sciences, University of Manchester, UK
R. WILLIAMS
Affiliation:
Department of Medical Physics, University Hospital of South Manchester, Manchester, UK
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Abstract

The crush model of injury in skeletal muscle is widely used in the investigation of tissue degeneration and regeneration. Previously, such trauma has been induced by using forceps to crush the muscle, commonly applying sufficient pressure to bring the mid-arms of the forceps together. This report introduces a reliable electromechanical device designed to generate reproducible focal lesions in skeletal muscle of mice. The tibialis anterior was crushed in 17 young adult mice. Two days after injury, the muscles were examined microscopically. By morphometric analysis, it was determined that the volumes of the lesions produced were similar (mean 0.499 mm3±0.098, range 0.278−0.601 mm3), and that the full extent of the damaged muscle was easily distinguished and readily quantifiable. This will allow a more precise comparison in future investigations into regenerative differences between age groups, satellite cell activation and the inflammatory response.

Keywords

Mousemuscle crush injury
Type
Research Article
Information
The Journal of Anatomy , Volume 190 , Issue 3 , April 1997 , pp. 417 - 422
Copyright
© Anatomical Society of Great Britain and Ireland 1997

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