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Macrophages and apoptosis in the stellate reticulum of the rat enamel organ

Published online by Cambridge University Press:  19 October 2000

LIRIANE BARATELLA ,
VICTOR E. ARANA-CHAVEZ  and
EDUARDO KATCHBURIAN
LIRIANE BARATELLA
Affiliation:
Department of Biology, University of Ponta Grossa, PR, Brazil Department of Morphology, School of Medicine, Federal University of São Paulo, São Paulo, SP, Brazil
VICTOR E. ARANA-CHAVEZ
Affiliation:
Department of Histology and Embryology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil
EDUARDO KATCHBURIAN
Affiliation:
Department of Morphology, School of Medicine, Federal University of São Paulo, São Paulo, SP, Brazil
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Abstract

Tooth germs of upper first molars of 1, 3, and 5-d-old rats, fixed in formaldehyde, were stained for the detection of apoptosis by the TUNEL method, and by the azo-dye method for the demonstration of acid phosphatase. For conventional light and electron microscopy the specimens were fixed in glutaraldehyde- formaldehyde and embedded in glycol methacrylate and Araldite. Results showed that macrophages, present in the stellate reticulum, contained basophilic bodies and TUNEL-positive globules, i.e. apoptotic bodies, in their interior. Macrophages also possessed strong acid phosphatase activity. Electron microscopy showed the presence of large vacuoles inside the macrophages containing dense fragmented material. Taken together these results suggest that the intra-epithelial macrophages of the stellate reticulum engulf apoptotic bodies.

Keywords

Tooth germamelogenesisodontogenesisenamel
Type
Short Report
Information
The Journal of Anatomy , Volume 197 , Issue 2 , August 2000 , pp. 303 - 306
Copyright
© Anatomical Society of Great Britain and Ireland 2000

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