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Confocal laser scanning microscopy of porcine skin: implications for human wound healing studies

Published online by Cambridge University Press:  01 June 1997

N. J. VARDAXIS ,
T. A. BRANS ,
M. E. BOON ,
R. W. KREIS  and
L. M. MARRES
N. J. VARDAXIS
Affiliation:
Department of Medical Laboratory Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
T. A. BRANS
Affiliation:
Burns Unit, Red Cross Hospital, Beverwijk, The Netherlands
M. E. BOON
Affiliation:
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands
R. W. KREIS
Affiliation:
Department of Medical Laboratory Sciences, Royal Melbourne Institute of Technology, Bundoora, Victoria, Australia
L. M. MARRES
Affiliation:
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands
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Abstract

The structure of porcine skin as examined by light microscopy is reviewed and its similarities to and differences from human skin are highlighted. Special imaging techniques and staining procedures are described and their use in gathering morphological information in porcine skin is discussed. Confocal laser scanning microscopy (CLSM) was employed to examine the structure of porcine skin and the findings are presented as an adjunct to the information already available in the literature. It is concluded that CLSM provides valuable additional morphological information to material examined by conventional microscopy and is useful for wound healing studies in the porcine model.

Keywords

Pontamine sky blue stainMIB-1collagenelastin
Type
Research Article
Information
The Journal of Anatomy , Volume 190 , Issue 4 , June 1997 , pp. 601 - 611
Copyright
© Anatomical Society of Great Britain and Ireland 1997

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