In dealing with fertility in sheep there are a number of initial difficulties. On the one hand, there is generally a lack of any uniform definition of such terms as ‘fertility’, ‘fecundity’ and ‘prolificacy’ (cf. Marshall & Hammond, 1947; Lush, 1938; Rice, 1942; Asdell, 1946; and others); on the other hand, investigators have been confronted with a real difficulty in procuring suitable information which would conform to the requirements of any single and adequate definition of fertility. Because of this, fertility figures have been calculated in different ways* and may differ by as much as 30%. The situation is at present so confused that reference to similar work, especially when the original papers are not available, or the terms not clearly denned, is often of dubious value if not altogether misleading. In the past the data analysed were mostly based on farmers' answers to questionnaires or on flock records. The limitations of such methods are only too obvious; yet it must be clearly realized that in commercial flocks there is no possibility of any basic departure from them, even though their accuracy could in many cases be improved. In other words, only such information is collected as the circumstances allow. This, for instance, may be the number of lambs docked per ewes put to ram in one flock and number of lambs docked per ewes lambed in another.

1. Studies were made of the effects of varying ground water-levels upon the productivity and composition of Italian Ryegrass grown on a calcareous light peat in the Fenland area. Six cuts were taken throughout the season at 3 to 4-weekly intervals.

2. High ground water-level (approximately 15in. below ground surface) had a very deleterious effect on the total yield of fresh grass and of dry matter. Yields were little more than half of those obtained at medium and low water-levels (24 and 38 in. below ground surface, respectively).

3. High water-level apparently interfered with nitrogen metabolism in the soil, and considerably lower percentages of crude protein were found in the grass growing on the high water-level plots than at the other levels. On the other hand, the percentage of crude fibre remained fairly constant for all levels of ground water.

4. High water-level also had a depressing effect on the percentage of potassium, magnesium and chlorine in the grass. It had no obvious effect upon the calcium and phosphorus levels in the plants. The silica content of the grass rose steadily as the season advanced, this being most marked on the high water-level plots.

5. Physical examination of typical plants showed the effect of the various ground water-levels upon the development of the root systems, with consequent effect upon the chemical composition of the grass.

6. The composition of hay and aftermath showed similar changes to those reported for the green herbage.

7. The findings are discussed in relation to grassdrying policies in Fenland areas.

The influence of growing plants on nitrification in the soil was studied by means of small lysimeters of which four were planted to a perennial grass, four to an annual millet crop and four were left fallow.

Nitrification was entirely repressed under the grass from the second season after its establishment onwards, and did not take place even when the grass was dormant in winter. This was due to a direct influence of the living root, since in the fallow soil which was treated similarly, nitrification took place freely throughout the winter. Under the annual crop a repression of nitrification could be detected only towards maturity of the crop and the soil solution was completely depleted of nitrates at this period. Nitrification was resumed, however, immediately after the crop was ripe and had died off and continued through the winter.

During the period that nitrification was depressed replaceable ammonia made its appearance in the soil in more than normal quantities. This fact is hold to indicate that the plant exerts its influence on the mineralization of nitrogen in the soil by paralysing the autotrophic dehydrogenase system of the nitrifying organisms without interfering with the process of ammonification and not, as has been claimed, by excreting such quantities of carbonaceous matter that nitrates are reassimilated by micro-organisms.

By virtue of the constancy of the carbon-nitrogen ratio in soils this influence of plants on the mineralization of nitrogen has a very important bearing on the conservation of soil humus and consequently on any system of alternate husbandry. Some of its implications were discussed with particular reference to local fertilizer practice and field experience.

An attempt has been made to evaluate the role of seven ‘ultra-simple’ seeds mixtures in the production of pasture during the grazing season. The individual value of each mixture is assessed and its period of production described.

Pasture output was measured in dry matter and protein yields along with live-weight gains obtained with fattening cattle.

In the course of two breeding seasons 137 mature to aged ewes of mixed breeds but predominantly Border Leicester × Cheviots, Dorset × Cheviots, Suffolks and Hampshires and their crosses, have been injected with PMS or PU, and at different levels of dosage, and slaughtered at intervals after service to determine the sequence of events following treatment. In addition ten ewe lambs received similar treatment and eighty-one flock Suffolk and Romney Marsh ewes, of which forty-nine were injected, have been studied. The results may be summarized as follows:

1. The injection of 500–2000 i.u. PMS on the 12th day of the oestrous cycle will regularly induce multiple ovulations at the ensuing heat.

2. There is a significant dose-response relationship between 500 and 2000 i.u. PMS. Mean ovulation rates observed were: 500 i.u., 4·1; 1000 i.u., 10·6; 2000 i.u., 15·8. There is, however, considerable variation, the ranges being 2–9, 4–33 and 8–29 respectively.

3. The injection of 1000 i.u. PU subcutaneously on the 12th day of the cycle causes marked ovarian and cyclic abnormalities, and heat may be entirely suppressed. When injected intravenously at heat following a priming injection with PMS on the 12th day there is no evidence that it increases the rate of ovulation; in other words, the ewe can ovulate up to at least thirty ova without difficulty, provided the follicles are matured.

4. PMS levels of 500 and 1000 i.u. do not cause any apparent ovarian abnormalities apart from superovulation. A very high proportion of all follicles developed rupture and form apparently normal corpora lutea. PMS at 2000 i.u. causes lutein cysts and ovulation may be inhibited.

5. PMS at 500 and 1000 i.u. causes no cyclic abnormalities, although cycle length is slightly shortened. In the event of the ewe failing to conceive, the subsequent oestrus is normal, and ovulation and fertilization of the ova occur.

6. Commercial PMS is probably as effective as fresh PMS provided it is correctly standardized. There seems no reason to suspect differences in multiple ovulating efficiency between different batches of fresh PMS.

7. While breed differences in response possibly exist they were not observed in these experiments. Nor were differences apparently related to the relative time of injection within the breeding season.

8. There is an ovarian weight-PMS level, doseresponse relationship which is almost entirely accounted for by the numbers of corpora lutea and hence of luteal tissue.

9. Multiple-ovulated ova are highly fertilizable. However, when fifteen or more are shed the rate of tubal transport is considerably accelerated and the proportion fertilized appears to fall. One- and two- cell ova have been recovered from the uterus within 48 hr. of service. Nine fertilized ova have been recovered from one ewe.

10. Considerable embryonic mortality occurs before attachment of the blastocysts. None the less, multiple impregnation does occur but is followed by further early death. Up to thirteen attachments have been observed in one ewe, but all but three were showing signs of regression by the 19th day.

11. Post-attachment mortality takes the form of an initial retardation commencing about the 15th day, embryos apparently dying a day or so later. The peak of mortality occurs between the 17th and 19th day, by which time definite signs of resorption are apparent.

12. By 21 days equilibrium has been reached, the mean numbers of survivals of those ewes pregnant being some 260%. This level is maintained without further loss until the 68th day. Several cases of four normal foetuses were noted up to the 41st day, and one uterus containing six perfectly normal foetuses was recovered at 61 days.

13. The overall fertility indicated by these slaughterhouse ewes varies with the level of PMS administered. While equilibrium is achieved by animals conceiving, at about 250–260% viable foetuses, regardless of the dose injected, there is increasing proportion of returns to service with increasing dosage. For the most part this appears due to hormonal imbalance at the time of ovulation and fertilization when an excessive number (> 15) ova are shed. This results in accelerated ovum transport through the tubes and lowered rate of fertilization. Between ovulation rates of 4 and 12, produced by 500 i.u., rate of conception is extremely high. Of twenty-five ewes receiving 500 i.u. PMS, twenty-three (92%) conceived to the first fertile service. The conception rates were reduced to 80 and 60% by 1000 and 2000 i.u. respectively.

14. Although after the end of the 3rd week there is no direct evidence of further foetal mortality, the percentage of lambs born in the flock ewes injected with 500 i.u. PMS was only 192% of those lambing or 167% of all ewes mated (147% for controls). This indicates a loss late in pregnancy. This can in part accounted for by abortion; one ewe aborted, due, is believed, to her carrying more lambs than she was physically capable of retaining.

15. OffifteenflockSuffolksinjectedinthe 2nd year with 500 i.u. PMS, thirteen lambed, one aborted and one did not conceive. The thirteen ewes lambing presented twenty-five lambs, including one set triplets and one of quadruplets. All the latter were viable, but two sets of twins were bom dead. One triplet was lost on fostering and one other lamb was lost, so nineteen were tailed. Of fifteen controls, all lambed, giving twenty-one lambs bom of which twenty survived. Conception to first fertile service was higher in the injected than in the control ewes. In the preceding year when 1000 i.u. was given, both conception and lambing rates were appreciably lowered as compared with untreated controls. The significance of this in respect to the level of PMS administered is stressed.

16. It is concluded that the major part of the embryonic loss is due to a uterine environment which is incapable of supporting more than a limited number of embryos. It is considered possible that there are breed differences in this, and that each breed, and individual within the breed, has ‘Maximum Potential Fertility’.

17. This ‘Maximum Potential Fertility’ is not attained in normal breeding practice, since the number of ova shed by the ewe tends to constitute a limiting factor. Use of a level of PMS which will induce between three and ten ovulations—in the case of this experiment, 500 i.u.—removes this limiting factor, giving the individual full opportunity to express its full potential breeding capabilities.

18. There is some indication that the use of PMS in lambs may not give good results, and its use for the while should be restricted to mature ewes.

19. Since 500 i.u. PMS has resulted in a higher conception rate to first service and to a greater number of lambs born, it is apparent that this technique of injecting on the 12th day of the cycle a level of PMS which will result in a moderate number of ovulations is worthy of exhaustive field trials, notwithstanding the high post-natal mortality observed in the few cases which were allowed to go to term.

20. In view of the higher indicated fertility of the slaughtered ewes which were mainly Border Leicester cross and Dorset × Cheviots—normally very highly fertile ewes as compared with the flock Suffolks—it is considered possible that this technique will yield best results with more highly fertile breeds.

21. To have any hope for successful increase of fertility in mammals following gonadotrophin treatment the hormone must be administered at a time and at such a concentration that it will augment, but not upset, normal hypophysial and ovarian function.

1. Chicks from commercial sources were housed intensively and fed on a series of rations containing decorticated extracted groundnut meal as the main supplementary source of protein, with and without additions of animal by-products and aureomycin. Parallel groups received rations conforming to Government regulations in their content of sources of animal protein.

2. Growth and feed efficiency up to point of lay were as good in groups fed on all-vegetable rations, containing 18% of crude protein in the starting ration and 16% in the growing ration, as in groups fed on regulation rations of the same protein content.

3. Performance on the all-vegetable ration up to 6 weeks of age was not as good as on the regulation ration. This inferiority could be largely or wholly corrected by adding extra groundnut meal and condensed fish solubles to the ration. Growth and feed efficiency over the whole rearing period were not improved by the addition of these supplements.

4. Addition of aureomycin stimulated growth over the whole rearing period but failed to improve feed efficiency.

5. Protein levels in the ration, the provision of animal protein factor(s) and aureomycin were without effect on the age at which laying began.

6. Current estimates of the chick's requirement for certain amino-acids appeared to be too high when this type of ration was used.

7. The addition of riboflavin to these starting rations was essential. When this was done mortality was low, leg weakness absent and feathering rate normal.

Mating Wessex Saddleback with Gloucester Old Spot pigs shows clearly that the former are of the three genotypes expected if the belt pattern is primarily determined by a single dominant gene (Be). Breeding tests and measurements of belt width agree in showing that homozygotes tend to have medium to wide belts, heterozygotes tend to have medium, narrow or broken belts, while pigs homozygous for the recessive are black. Crosses of Wessex with black-spotted pigs produce black piglings with or without wide symmetrical belts depending on the genotype of the Wessex parents.

Selection of breeding pigs with narrow belts maintains a high incidence of recessive blacks. Solid blacks which are nevertheless genetically belted may occur, but if so their numbers are likely to be very small.

Evidence is given which suggests that pigmentation in the skin and hair of Wessex × Large White pigs is much reduced if the Large White parents have blue eyes.

An attempt was made to determine what becomes of the nitrogen added to a poor soil in moderate quantities of organic manures or of ammonium sulphate. Losses by drainage, in two successive barley crops, and by fixation in an unavailable form in the soil were determined, and it was found that with every material used (including tare residues containing 3½% nitrogen, mustard plant residues containing 1½–2¼ nitrogen and pure ammonium sulphate) and with intensive cropping and leaching, not more than 40–51% of the added nitrogen could be accounted for at the end of the experimental period. The balance, it is suggested, must have disappeared from the system, possibly as gaseous nitrogen.

Incidentally to the above, it would appear that after the first crop of barley the presence of the manurial additions of tare residues, and especially of ammonium sulphate, tends to hinder the formation of leachable nitrogen in the soil.

1. The experiment previously described (series 4, Holmes, 1949) on the effect of massive applications of nitrogenous fertilizers on the productivity of a ryegrass dominant pasture was continued for 3 years (1946, 1947, 1948). The manurial treatments ranged from none to the application of 312 lb. nitrogen per acre and this was applied with and without 135 lb. P2O5 and 168 lb. K2O per acre. Farmyard manure was applied to one block in 1948.

2. With the heaviest nitrogen treatment plus phosphate and potash the average yield for 3 years was 8000 lb. dry matter and 1640 lb. crude protein (similar to the yield in 1946) compared with a control yield of 4720 lb. dry matter and 590 lb. crude protein. The yields declined from year to year when phosphate and potash were not applied, the decline being greatest with the heaviest application of nitrogen.

3. The seasonal distribution of the yield of herbage was very considerably modified by the time when fertilizers were applied.

4. The botanical composition of the swards was related to the yield, 70% of the grasses in the highest yielding sward being perennial ryegrass and cocksfoot while the lowest yielding sward contained only 35% of these grasses.

5. The manurial treatments had no effect on the pH, loss on ignition or the content of readily soluble P2O5 in the soil, but in the first year there was some evidence of a general reduction of readily soluble K2O to a low level.

1. The structure of the cattle population (4 June returns), as well as of cattle sales to the Ministry of Food, have been illustrated.

2. The trends in cattle production show the definite changes from that of the traditional storestock rearing to that of milk selling.

3. A considerable part (38·2%) of the total graded animals slaughtered for beef are the ‘wastage’ animals from the breeding and dairying herds.

4. Reference has also been made to the high proportion of ungraded cows (36·7% of the total cows) purchased by the Ministry. The ungraded cattle, however, are only 17% of the total sales. On the other hand, the ungraded ‘wastage’ cattle contribute 90% of the total ungraded cattle.

5. More heifers than steers were sold for slaughter, and it has been shown that the average live weight of the graded steers is nearly 224 lb. heavier than that of the heifers.

1. A small-scale plot experiment was carried out from 1947 till 1949 to study the effect of massive dressings of nitrogen with and without phosphate and potash on the yield of a ryegrass-dominant sward. A 4 × 4 Graeco-Latin square was used.

The nitrogen treatments were: (1) no nitrogenous fertilizer, (2) 260 lb., (3) 520 lb., (4) 416 lb. nitrogen per acre per annum. These applications were divided into four or five equal dressings, the first being applied in March and the others after successive cuts.

In 1948 and 1949 the following mineral treatments were also applied: (A) no mineral fertilizer, (B) 180 lb. K2O per acre, (C) 120 lb. P2O5 per acre, and (D) treatments B and C combined. These treatments were applied in two parts, half in spring and half in midsummer.

2. Applications of phosphate did not affect the yields of herbage but yields were severely restricted in the absence of potash.

3. Where potash was applied there was no significant change in the annual yield of herbage from 1947 to 1949 for any one of the treatments. The nitrogen treatments increased the yields from 4300 lb. dry matter and 600 lb. crude protein per acre with treatment 1 to 9000 lb. dry matter and 1900 lb. crude protein with treatment 3. The yield response of dry matter to nitrogen applications was curvilinear, but that for crude protein was rectilinear.

4. Increasing the application of nitrogen per cut from 52 to 104 lb. per acre raised the protein content in the dry matter of the herbage from 15·20 to 18·25%.

5. The plots receiving the nitrogen treatments gave earlier production and also produced a greater proportion of the total yield in July-September than the untreated control plots.

6. Where potash was present the average net efficiency of recovery of nitrogen for 3 years was 47, 39 and 37% for treatments 2, 3 and 4 respectively.

7. Where potash was present the content of vigorous grasses in the sward (perennial ryegrass and timothy) was increased in relation to the quantity of nitrogen applied, from approximately 40% in treatment 1 to 75% in treatment 3. The content of vigorous grasses in the sward was reduced in the absence of potash.

8. The results and their practical implications are discussed.

Designs are given for factorial experiments with three, four or five factors at two levels each, using thirty-two plots in a 4 × 8 lay-out on the ground. The effects of both rows and columns can be eliminated from the estimate of error. Provided that three-factor interactions can be ignored, information can be retained on all two-factor interactions.

1. The relation between the soluble-nitrogen content of grass silage and the pepsin digestibility has been investigated, and formulae have been derived for calculating the protein digestibility in the silage dry matter from the determined solublenitrogen content.

2. Comparisons have been made between the calculated results for protein digestibility, the results using the formulae of Watson and Dijkstra and the digestibility as determined on sheep.

3. The peculiarities of molassed silage in the above connexion have been noted.

1. Differences in cattle population (4 June returns) and in average body size of fat cattle (data from the collecting centres) have been illustrated for the three counties (Cardiganshire, Carmarthenshire and Pembrokeshire) constituting this region. They indicate the superiority of Pembrokeshire.

2. The six westerly collecting areas of Pembrokeshire produce the heaviest cattle of better grading. It has a higher proportion of steers to heifers than the rest of the region.

3. The statistical analysis of the data has made it possible to partition the region into four main groups of centres, which show significant differences in the size of the fat cattle.

4. The superiority of group 1 has been demonstrated in relation to (a) the seasonally of deliveries, (b) the percentage grading and (c) in the average live weights.

5. These differences are examined in greater detail by comparisons of the deliveries to the Pembroke and to the Llandilo centres. These show that Pembroke is superior in average live weights, percentage grading and in the proportion of steers to heifers.

6. The ungraded cattle are also examined, and the results indicate the same trends within the region as for the graded cattle.

7. The possible explanation of the variations or the ecology of beef production has been discussed in the light of Ashton's (1930) suggestions that variations in body size between cattle of different breeds is mainly due to the calcium and phosphorus content of the soil.

8. The conclusion is drawn that differences of climate dominate the whole picture, because of the effect of rainfall on leaching and soil erosion, as well as on the quality of the harvested fodders, the effect of temperature on the length of the growing season and of the beneficial influence of sunshine on the quality of both grass and hay.

1. A statistical study of the causes of variation in milk yields has been made on the basis of 5000 lactation records from twelve leading herds of Ayrshire cattle in south-west Scotland for the period 1930–9.

2. The unit of measurement of milk production employed was the yield during the first 180 days of the lactation period. This eliminated the effect of variations in length of current calving interval on milk yield.

3. The effect of month of calving on milk yield varied significantly between herds, and it was shown that correction factors for month of calving should be calculated on a within-herd basis. The average difference in 180-day yield between the summer and winter calvers of all herds was about 10% in favour of winter calvers.

4. The milk yield of a cow was found to be influenced both by the number of her previous lactations and also by her age at calving. The types of corrections for age employed by previous investigators were discussed, and it was shown that percentage corrections are the most satisfactory.

5. There was a positive correlation between milk yield and length of preceding calving interval. From an economic point of view, the optimum length of calving interval was about 400 days for the first lactation, and 1 year for subsequent ones. Corrections for preceding calving interval, like those for age, were most satisfactory when they were proportionate and not additive.

6. No significant differences were found among the first three records of a cow in their ability to indicate her actual production capacity. The probable performance of a cow in any lactation was predicted as accurately from the lactation immediately preceding it as from the average of a number of previous lactations.

7. The average repeatability of milk yield was 0.46, and heritability was of the order of 0.25–0.30.

8. The probable effect on herd improvement of selecting breeding females was found to be very small, extremely little genetic progress being attained by this method in the twelve herds.

1. A statistical study of factors affecting variation in persistency of lactation has been made using 5000 lactation records from twelve leading herds of Ayrshire cattle in south-west Scotland for the period 1930–9.

2. It is suggested that a satisfactory numerical expression for the shape of the lactation curve could be obtained from the formula, Persistency = where A is the milk yield during the first 180 days and B is the initial milk yield, namely, the milk yield during the first ten weeks of lactation.

3. The interrelationships of persistency, initial milk yield and 180-day milk yield showed that these three characteristics are positively correlated with one another on a between cows within herd basis. This meant that it should be possible to combine high initial milk yield with high persistency to obtain increased total production.

4. The effect of month of calving on persistency of lactation varied significantly between herds. On the average, the highest persistency was attained by cows calving in the winter, and the lowest by summer calvers.

5. The variation of persistency with age showed that it is necessary to correct for the high persistency of first calvers when comparing cows with different lactations.

6. The average repeatability of persistency was 0.242 in the present material, while heritability was of the order of 0.10–0.15.

7. It has been concluded that improved feeding and management would bring about the greatest returns in the direction of improved persistency.

During the period 1942–50 some thirty-seven multifactorial field experiments have been carried out to determine the conditions under which weeds in fibre flax can be selectively controlled by herbicides. In order to assess the direct effects of treatment on flax, some experiments have been undertaken in weed-free crops, while others have been sited in weed-infested crops so that the increases in productivity following on weed suppression could be evaluated.

In almost all experiments, the total crop weight, the yield of de-seeded straw and the amount of scutched fibre per plot were determined, while in a proportion seed yield was also measured. Where weeds were present, counts were made to establish the degree of control.

The preliminary experiments demonstrated that sulphuric acid, ammonium sulphamate and ammonium thiocyanate were far too toxic to flax. An examination of a range of cupric salts suggested that the most promising compound was cupric chloride, while of the formulations of dinitro-o-cresol investigated only the sodium salt appeared to be sufficiently selective. It was also found that in terms of fibre production sodium 2-methyl-4-chlorophenoxyacetate was less toxic than sodium 2:4-dichlorophenoxyacetate. In consequence, the bulk of the investigations have related to determining the optimal conditions for the use of cupric chloride, sodium dinitro-o-cresylate and sodium methylchlorophenoxyacetate.

For all three compounds it has been observed that flax is least liable to injury at a height range of 2–6 in. There are limitations to the amounts which can be safely employed, namely, 20 lb. of cupric chloride or 6 lb. of sodium dinitro-o-cresylate applied in 100 gal. of spray solution per acre. With sodium methyl-chlorophenoxyacetate the corresponding limit is 1 lb./acre, and the evidence suggests that the crop may be injured if the volume of application is reduced below 25 gal./acre.

Within these limitations of concentration volume and stage of development of the crop, the suppression of annual weeds has led to significant increases in the yield of straw, seed and scutched fibre. Larger gains resulted from the use of sodium methyl-chlorophenoxyacetate and sodium dinitro-ocresylate. This superiority over cupric chloride is in part attributed to the wider range of weed species which are killed by the two sodium salts and in part due to the greater liability of crop damage if the limits on amount and time of application are not strictly observed.

The results have also clearly established that for fibre flax the weight of total crop, the yield of straw, the amount of seed or plant height cannot be taken as reliable criteria for the evaluation of herbicides, for while all these may be little affected by treatment yet there may still be large reductions in the amount of recoverable fibre. A preliminary anatomical examination of the internal changes in the stems of flax has shown that when treatment with dichlorophenoxyacetic acid is delayed, a marked but localized curvature of the stem results and in this zone fibre formation is abnormal: thus breakages either during pulling or processing may occur at this point of weakness.

Between 1941 and 1946 some twenty-five field experiments were carried out at various centres in England to assess the factors which determine the yield level and seed composition of linseed (oil flax). The experiments were of multifactorial design, and the main variables studied were varietal differences, levels of mineral nutrient supply and plant population. Additions of nitrogen (35 lb./acre) over all experiments raised the yield of seed by 10%, whereas additional phosphorus (50 lb. P2O5/acre) or potassium (80 lb. K2O/acre) had small and inconsistent effects. A significant interaction between nitrogen and variety was recorded in one trial, but in twelve out of fourteen experiments the varieties reacted uniformly to a combined application of nitrogen, phosphorus and potassium, while in the remaining two experiments the interactions were contradictory.

1. Trials made near Cambridge in 1949 and 1950, under ‘mild’ and ‘hard’ spring weather conditions respectively, have confirmed that the findings of Aberg & Denward in Sweden, and of Pedersen, Andersen & Hermansen in Denmark, concerning distortions in barley caused by MCPA and 2,4-D, are also valid under English conditions.

2. Damage to barley caused by the application of MCPA and 2,4-D up to the 2-leaf and early 3-leaf stages may be recognized by the occurrence of many tubular leaves and bowed or trapped ears. As little as 2 lb./acre of 2,4-D, as the amine salt, applied at this stage may also cause severe stunting of theplants with distortion of the roots.

3. Damage to barley by the application of MCPA and 2,4-D at the 3–4-leaf stages may be recognized by the occurrence of tweaked ears. The application of as little as 2 lb./acre of MCPA, as the sodium salt, at these stages may cause tweaking in over 50% of the ears. 2,4-D (as amine) at the same dosage causes more distortion. Although the reduction in yield due to such tweaking may be slight, the grain sample is irregular in size, with a number of naked grains, and malting quality may be reduced.

1. On the basis of 5000 records from twelve leading herds of Ayrshire cattle in south-west Scotland for the period 1930–9, a statistical study has been made of some of the causes of variations which occur in butterfat percentage.

2. Butterfat percentage was found to be affected only very slightly by month of calving, age and length of calving intervals.

3. Selection for milk yield would not affect butterfat percentage adversely to any appreciable extent.

4. The average repeatability of butterfat percentage was 0·691 in the present material, and heritability was of the order of 0·50–0·60.

5. Mass selection would be an effective tool in bringing about improvement in butterfat percentage.