Data from several field experiments (eight crops grown under a widely varying nitrogen supply on a loess loam soil) were used for a simulation modelling based analysis of nitrogen availability of cauliflower. The model was built out of components describing root growth, nitrate transport to the roots and the vertical nitrate transport within the soil.

Root observations obtained over 2 years indicated an increased fraction of dry matter allocated to the fine roots under N deficiency. An adopted version of a root growth model for cauliflower described the rooting data with an R2=0·75. Based upon an acceptable description of the soil water budget, vertical nitrate movement during the growth period of cauliflower was accurately described. The magnitude of this movement, however, was limited to soil depths of about 60 cm even after periods of high rainfall, because of a high soil water holding capacity. An analysis of the factors determining nitrate availability indicated that apparent mass flow was only of high importance for conditions of extremely high N supply where high amounts of nitrate nitrogen remain in the soil up to the end of the growing season. Otherwise, the dominating fraction of nitrate has to be transported to the roots by diffusion. Single root model based calculations of maximum nitrate transport to roots overestimated N availability as indicated by estimates of critical soil nitrate N that were too low. The introduction of a restricted uptake activity period of the roots was used to bridge the gap between theoretical calculations and empirical results. Scenario calculations were carried out to obtain functional relationships between N supply and residual soil nitrate levels for different soil conditions and management practices.

Based on studies concerning dry matter (DM) partitioning, DM production, root growth, nitrogen (N) contents of cauliflower organs and soil nitrate availability (first part of the paper Kage et al. 2003b), an integrated simulation model for the cauliflower/soil system is constructed, parameterized and evaluated.

Dry matter production of cauliflower is described and predicted using a simple light use efficiency (LUE) based approach assuming a linear decrease of light use efficiency with increasing differences between actual, NCAProt, and ‘optimal’, NCAoptProt area based leaf protein concentrations. For 2 experimental years the decline of LUE with decreasing nitrogen concentration was at 0·82 and 0·75 (g DM×m2/(MJ×g N)). Using the parameters obtained from the first experimental year shoot DM production data of cauliflower from five independent experiments with varied N supply containing intermediate harvests could be predicted with a residual mean square error (RMSE) of 72 g/m2 for intermediate harvest DM values ranging from about 50 to 900 g/m2. Nitrogen uptake and partitioning of cauliflower was simulated using functions describing an organ size dependent decline of N content. Leaf nitrate was considered explicitly as a radiation intensity dependent pool, mobilized first under N deficiency. The curd was assumed to have a sink priority for nitrogen. The model predicted shoot N uptake including data of intermediate harvest with a RMSE of 2·4 g/m2 for intermediate harvest N values ranging from about 3 to 30 g/m2. Nitrogen uptake of cauliflower at final harvest was correlated to final leaf number.

A scenario simulation was carried out to quantify seasonal variation in N uptake of cauliflower cultivars under unrestricted N availability. Due to variations in the length of the vernalization phase, simulated shoot N uptake ranged from about 260 kg N/ha for spring planted crops to about 290 kg N/ha for summer planted crops of the cultivar ‘Fremont’. The cultivar ‘Linday’, which shows a more severe delay of vernalization under high temperatures, shows on average a larger shoot N uptake for summer planted crops of about 320 kg N/ha and a much larger variation of shoot N uptake.

Durum wheat genotypes with different responses to moisture stress were studied in a glasshouse under moisture stress and non-stress conditions to investigate differences in water use and transpiration efficiency and interrelationships among water use and transpiration efficiency and associated traits. Significantly high genotypic variability in the cumulative amount of water used before (ETba) and after (ETpa) anthesis was observed. Susceptible genotypes used higher amounts of water before anthesis and lower amounts after anthesis. In contrast, tolerant genotypes used a higher proportion of water during the post-anthesis period. Significantly high variability among the genotypes was observed for various measures of water use and transpiration efficiency, total dry matter and harvest index. Ranking of cultivars for water use efficiency based on grain yield (WUEG) and transpiration efficiency based on grain yield was consistent with ranking of cultivars for drought susceptibility indices. Drought susceptibility index was significantly but negatively correlated with harvest index, WUEG and grain yield. However, it was positively and significantly correlated with the ETba[ratio ]ETpa ratio. A high positive correlation of WUEG with harvest index and grain yield with harvest index was found. It was concluded that selection for lower ETba[ratio ]ETpa ratios up to about 0·8 could indirectly lead to improved WUEG and HI, hence improved grain yield. Selection for increased WUEG and/or grain yield would increase yield in water-limited environments.

The risk of drought is high in the Sudan savannah zone of West and Central Africa because rainfall in this area is unpredictable in quantity and distribution. Thus, improved maize genotypes tolerant to drought could stabilize maize grain yield in this zone, where recurrent drought threatens grain production. Six maize genotypes, two each of hybrids, open-pollinated varieties (OPVs) and landraces, were evaluated for tolerance to terminal water deficit before flowering. Water deficit significantly reduced growth, grain yield and yield components of the maize genotypes. Significant differences were observed among genotypes for all the traits measured. One hybrid, 9011-30, and two improved OPVs, STR-EV-IWD and IYFD-C0, that showed tolerance to water stress recorded higher grain yield, and accumulated and partitioned more assimilates to the grain than the drought-susceptible genotypes. Also the drought-tolerant genotypes, 9011-30, STR-EV-IWD and IYFD-C0 had more ears/plant and greater numbers of kernels/ear. These genotypes could serve as sources of drought tolerance for the development and improvement of new drought-tolerant maize genotypes.

Physiological traits responsible for differences in yield performance among pre-released genotypes are normally not known. The objective of the present study was to evaluate seasonal variations in dynamic growth and development traits between 12 large-seeded Virginia-type and 14 small-seeded Spanish-type advanced breeding lines of peanut (Arachis hypogaea L.). The experiment was conducted in 1999 and 2000 at Khon Kaen University in Northeast Thailand for three planting dates, representing the early-rainy season, mid-rainy season and dry season. Very similar phenological development was observed for all lines within each group and between the early and the mid-rainy seasons. However, a lower temperature during the early growth stage in the dry season delayed flower initiation and extended maturity. During this period, crop growth rates as well as the development rate for leaf area index and specific leaf area were also lower compared with the rainy seasons. On average, both pod yield and total biomass for the rainy season and the dry season were about the same. Variations among lines within each group were also small. For these advanced breeding lines, the pod growth rate was the most important yield determinant while the crop growth rate had lesser effect and the partitioning coefficient and pod-filling duration had no significant effect. However, the crop growth rate, pod growth rate and partitioning coefficient were important traits for a line to be the top yielder. Pod-filling duration was also important when the lines involved were considerably diverse in maturity and seed size. Information on these traits, if used together with final pod yield, will make varietal selection more efficient.

Morphological description based on features of the olive stone, such as its surface and shape, can help to determine an olive cultivar's identity. The description, however, is based on visual examination and is thus affected by the examiner's expertise. Although the eye has the capacity to discern texture and shape, the values that are assigned to score different levels or descriptor states, such as a highly scabrous to smooth surface or a circular to elliptic shape, are categorical values. Studies on scoring methodology have shown that the assignment to categories or classes is problematic. The purpose of the present work was to classify olive cultivars by computer-image analysis of olive stone characteristics using mathematical tools, such as fractal geometry and moments. Fractals were used to extract texture information, and moments were used to extract shape information. The results revealed an overall classification accuracy of more than 90% using a Mahalanobis distance. The fractals and moments calculated for stones from genetically identical trees of the same cultivar did not show any statistically significant differences. As environmentally independent and robust morphological descriptors, both fractals and moments showed potential for accurate and efficient classification of olive cultivars and eventual description of olive diversity.

A stringent RAPD-PCR protocol was developed for reliable distinction at an early stage between somatic and doubled-haploid androgenic regenerates emerging from asparagus anther culture by using decamer primers with a high G/C content and/or primers with a dinucleotide sequence. RAPD-PCR was used to test 87 putative doubled-haploids from seven donor plants of different varieties and breeding lines, with differing genetic backgrounds. All of them differed from the anther donor, which suggests they had the desired doubled-haploid constitution, and demonstrates the effectiveness of the in vitro protocol used. This simple, reliable, fast, and comparatively cheap method eliminates the need for discriminating testcrosses and, thus, can significantly accelerate asparagus breeding programmes.

The influence of dairy cattle feed composition on the manure composition and on the dynamics and plant availability of cattle slurry N was studied. Dairy cows were fed seven different forages either with or without supplemental concentrates. The concentration of N in faeces dry matter varied from 18 to 38 g/kg dry matter and increased with increasing digestibility of the feed. Cattle slurries consisting of a mixture of 0·5 faecal N and 0·5 urinary N were stored according to common agricultural practice in Northern Europe. The mineralization of faecal N during slurry storage was very variable (0·09–0·50). The plant availability of N in the slurries originating from cattle fed with known diets was tested in small, framed field plots with spring barley, under conditions with minimal N losses. The nitrogen uptake in barley was determined and the mineral fertilizer equivalent (MFE) of slurry N was calculated. The net release of mineral N and CO2 from the slurries in soil was also measured in a parallel incubation study. The MFE of cattle slurry N varied from 53 to 75%. After correcting for the measured urine-N/faeces-N ratio and expected ammonia emission, the MFE varied from 51 to 78%. The plant availability and net release of cattle slurry N were influenced by forage type and feeding level. The MFE was negatively correlated with the concentration of crude fibre and neutral detergent fibre (NDF) in the diet, and positively correlated with the dietary protein content. The net release of CO2 from the slurries after 12 weeks in soil was significantly influenced by the concentration of crude fibre in the diet. The plant availability of slurry N was significantly correlated with the ammonium content (R2=0·53) and negatively correlated with the slurry C[ratio ]N ratio (R2=0·67) and the dry matter[ratio ]N ratio (R2=0·58). Residual slurry N left in the soil after harvest of the first crop varied from 0·25 to 0·47 of total slurry N. It is concluded that the fibre and the protein content of cattle diets have a significant influence on the plant availability of cattle slurry N and on the amount of residual slurry N remaining in the soil after the first growing season.

Management of semi-domesticated reindeer (Rangifer tarandus tarandus) in the northern regions have historically focused on reindeer–vegetation interactions, while few data on the food intake of these animals exist. Dry matter intake (DMI) was therefore estimated successively during winter in reindeer calves fed (i) ad libitum pelleted reindeer feed (RF-80; n=6), (ii) fed ad libitum mixed lichens (n=6) and (iii) in free-ranging reindeer calves (n=3) on natural winter pasture (March/April; 69 °N). Faecal output, determined with faeces collection bags, was 24±4, 6±3 and 22±3 g DM/day/kg BM0·75 in reindeer fed RF-80, mixed lichens and on winter pasture, respectively. Actual DMI of reindeer in captivity fed RF-80 and mixed lichens was 70±10 g DM/day/kg BM0·75 and 27±8 g DM/day/kg BM0·75, respectively. The DMI of the captive reindeer calves was accurately estimated on both diets on the basis of the digestibility of their diet and their faecal output. Using the same method, the DMI of free-ranging reindeer calves on natural winter pastures in northern Norway was estimated to be 34–61 g DM/day/kg BM0·75. The present study discusses factors that influence the food intake of reindeer in winter with emphasis on the seasonal regulation of appetite and DMI in these Arctic ruminants.

A comparative slaughter experiment was carried out to examine the effects of feeding either grass silage alone or a mixed diet of silage and concentrate on animal performance and the partitioning of nutrients between lean and fat deposition in steers. Eighteen Hereford×Friesian steers were randomly assigned to two dietary treatments; grass silage only (S) or a mixture of grass silage and a barley/soya bean meal concentrate (80[ratio ]20 on fresh basis) in the ratio of 60[ratio ]40 (on a metabolizable energy basis; SC), and to one of three target slaughter liveweights, 250, 350 or 500 kg. Metabolizable energy (ME) intake was maintained at 800 kJ ME per kg metabolic liveweight (kg0·75) per day. The relationships between chemical composition and empty body weight (EBW) at slaughter were assessed using allometric equations (logey=logea+blogeEBW). When assessed across the slaughter weights, supplementing silage with concentrates resulted in higher rates of liveweight (P<0·001), carcass fat (P<0·05) and protein (P<0·01) gains and a reduction in time taken to reach the average slaughter weight by 57 days (P<0·001). Carcass protein deposition was relatively linear across the slaughter weights (250–500 kg) and the relationship with EBW was Y=0·2372X0·8831 across treatments and did not differ between the diets. The rate at which carcass fat was deposited in relation to weight across all steers was Y=0·0004X1·9648 and was not different between the two diets. Hence, ratios of carcass fat[ratio ]protein and carcass fat[ratio ]protein gain ratios were not different. The results suggest that the main effect of feeding grass silage compared with grass silage-concentrate at similar levels of ME intake was to increase the rate of tissue accretion, but nutrient partitioning between fat and protein deposition was unchanged. There was no evidence of increased carcass fat[ratio ]protein deposition in silage-fed animals, which suggests that there is no problem of greater fat and reduced protein deposition in animals fed higher quality grass silage.

Calliandra calothyrsus is a tree legume native to Mexico and Central America. The species has attracted considerable attention for its capacity to produce both fuelwood and foliage for either green manure or fodder. Its high content of proanthocyanidins (condensed tannins) and associated low digestibility has, however, limited its use as a feed for ruminants, and there is also a widespread perception that wilting the leaves further reduces their nutritive value. Nevertheless, there has been increasing uptake of calliandra as fodder in certain regions, notably the Central Highlands of Kenya. The present study, conducted in Embu, Kenya, investigated effects of provenance, wilting, cutting frequency and seasonal variation both in the laboratory (in vitro digestibility, crude protein, neutral detergent fibre, extractable and bound proanthocyanidins) and in on-station animal production trials with growing lambs and lactating goats. The local Kenyan landrace of calliandra (Embu) and a closely-related Guatemalan provenance (Patulul) were found to be significantly different, and superior, to a provenance from Nicaragua (San Ramón) in most of the laboratory traits measured, as well as in animal production and feed efficiency. Cutting frequency had no important effect on quality; and although all quality traits displayed seasonal variation there was little discernible pattern to this variation. Wilting had a much less negative effect than expected, and for lambs fed calliandra as a supplement to a low quality basal feed (maize stover), wilting was actually found to give higher live-weight gain and feed efficiency. Conversely, with a high quality basal diet (Napier grass) wilting enhanced intake but not live-weight gain, so feed efficiency was greater for fresh material. The difference between fresh and wilted leaves was not great enough to justify the current widespread recommendation that calliandra should always be fed fresh.