A Phalaris tuberosa and Trifolium repens pasture was grazed continuously at stocking rates varying from 2·5 to 37·1 sheep per ha between 1964 and 1969. During this period herbage availability and composition, basal cover, root weight, water infiltration, soil moisture content, bulk density and chemical composition of the soil were measured at intervals.

As stocking rate was increased, herbage availability, root weight, basal cover, soil pore space and the rate of water infiltration declined, and bulk density and the nitrogen and calcium contents of the herbage on offer increased. In periods of below-average rainfall, soil moisture and nitrate levels were greater when herbage was of low availability.

Herbage production was calculated from estimates of herbage consumption and of litter decomposition, and averaged 8·45 t dry matter/ha/year; it was insensitive to changes in stocking rate over the range from 2 to 22 sheep/ha. The ratio, herbage consumption/ pasture production increased by 0'045 per unit increase in stocking rate.

Estimates were made at monthly intervals between January and December 1965 of the intake and nutritive value of the diet of unmated Merino ewes grazed continuously at stocking rates ranging from 2·5 to 37·1 sheep/ha. Oesophageally fistulated sheep were used to estimate intake and nutritive value.

Drought conditions existed during the experiment. Digestibility declined linearly with increasing stocking rate. The intercepts of the relationship between digestibility and stocking rate differed between months, being at a maximum in spring. Nitrogen content was also greatest in spring but did not change consistently with increasing stocking rate. Organic-matter intake declined linearly with stocking rate and increased asymptotically with total herbage available, the asymptotic relationship being the more precise. Neither relationship differed significantly between months. Organicmatter intake/ha increased continuously with increasing stocking rate and the maximum intake was predicted to occur at a stocking rate greater than that at which the sheep survived.

Wool production and wool characteristics are reported for a 5-year period in which sheep grazed Phalaris tuberosa–Trifolium repens pastures at stocking rates ranging from 2·5 to 37·1 sheep/ha. The utilization of nutrients for maintenance and wool production were calculated over shorter periods.

Maximum wool production/ha was always recorded at the highest stocking rate attained. The highest annual maximum was 111 kg clean wool/ha in 1963–4. The decline in wool production/sheep/unit increase in stocking rate was greatest during winter and the amplitude of the seasonal rhythm in wool production increased with stocking rate.

At high stocking rates wool count increased, staple length and character declined, colour and handle improved, soundness tended to decline and fibre entanglement (cotting) to increase particularly during drought. Live-weight gain/sheep also declined with stocking rate, the rate of decline being greatest during drought. Maximum live-weight change/ha was attained at a lower stocking rate than maximum wool production/ha.

The maintenance requirement of grazing sheep was estimated to be 58·3 kcal metabolizable energy/day/kg live weight and did not vary consistently with stocking rate. The value was 79% greater than the requirements for penned sheep estimated from fasting heat production.

Efficiency of wool production was defined as wool grown/100 g digestible organic matter intake (ED) and as wool grown/100 g nitrogen intake (EN). EN declined linearly with increasing stocking rate but the intercepts differed between months, and were least in late winter and early spring. En declined with stocking rate in winter and increased during summer. At low stocking rates, ED showed some variation throughout the year but this was much greater at high stocking rates.

Paspalum plicatulum was grown at Brisbane in boxes of sand receiving basal nutrients and frequent irrigation; weekly levels of ammonium nitrate application were varied according to growth and development stage.

The rate of tiller appearance increased to a maximum 40–50 days after sowing and almost ceased thereafter. Tiller leaf number, survival, fertility, inflorescence branching, seeds per raceme and seed size were positively related to tiller age. Young tillers were more sensitive to variations in nitrogen supply than old tillers.

Adequate nitrogen nutrition during the vegetative phase from sowing to floral initiation (93 days) increased tiller and hence inflorescence density; increased inflorescence branching was compensated by fewer seeds per raceme. Good nitrogen nutrition during the phase from floral initiation to inflorescence exsertion (142 days) increased survival of late-formed tillers and hence inflorescence density; inflorescence branching, seeds per raceme and seed size were also increased. Nitrogen stress during the final maturation phase did not affect seed yield.

Lower concentrations of the herbicide M15 stimulated the in vitro cellulose digestion and V.F.A. production by rumen micro-organisms. The herbicide Cotoran enhanced cellulose digestion but had no effect on V.F.A. production. The herbicide Dalapon decreased cellulose digestion and had little effect on V.F.A. concentrations. The herbicide 2,4·D had little or no effect on the in vitro cellulose digestion and V.F.A. production. The in vitro microbial activity was inhibited by the pesticide DDT. The effects of M15 2,4·D and DDT on V.F.A. production decreased with time. When the rumen fluid was fractionated, the V.F.A. production by the bacterial fraction was not significantly altered by the addition of 2,4·D and M15, whereas the activity of protozoal fraction was greatly enhanced. The response of V.F.A. production by washed suspensions of rumen ciliate protozoa to the addition of pesticides and herbicides was remarkably similar to that of rumen contents. Feeding M15 (60 ppm) increased the ruminal V.F.A. over those in the control sheep whereas 2,4·D had no or little effect.

Six successive trials with three adult rams (Sudan desert sheep) were conducted with the main object of finding a suitable index for the prediction of the nutritive value of protein in non-legumes for ruminants.

The mean change in the concentration of ruminal ammonia, blood urea and ruminal V. F. A. 3 h after feeding legumes was considerably greater than after feeding the nonleguminous hays. Among the legumes berseem hay gave the least change in the concentration of ruminal ammonia, blood urea and V. E. A. The leguminous hays produced more gas under in vitro conditions than the non-legumes. Berseem hay produced the greatest volume of gas. Butterfly pea hay and lubia hay gave more or less similar results. Among the non-legumes the desert grasses gave the lowest values.

Of all the feeds studied maize hay gave the highest nitrogen retention, followed by berseem hay. Despite this superiority of maize hay, the overall mean nitrogen retention of legumes was much greater than that of the non-legumes. Among the non-legumes dry desert grasses displayed a negative nitrogen balance.

Regression equations based on the present data indicated that nitrogen retention only of leguminous hays could be always predicted from changes in blood urea concentrations. The present results also showed that the nitrogen retention of non-legumes and to a lesser extent that of all feeds taken together (i. e. legumes and non-legumes) can be assessed by using volume of gas produced in vitro (i. e. rate of in vitro fermentation).

The percentage emergence from monogerm sugar-beet seed in field experiments was reduced by high soil moisture content and soil compaction, but was unaffected by soil temperature. Seed lots were differentially influenced when the soil conditions were very adverse. Pelleted seed emerged a little better in good soil conditions and was not more sensitive to high soil moisture content than raw seed.

The relation between laboratory germination and the number of emerged seedlings was variable and followed no obvious trends, and there was no consistent rank order in the emergence ability of four commercial cultivars.

Results of matings made in the Belmont breeding programme from 1954 to 1968 have been analysed. The analysis has been made within three groups. (1) The foundation cows in the years 1954–9; (2) the F1 generation which were mated within line in the years 1957–62; (3) the F2 and F3 generations which were mated within Africander cross (AX), Brahman cross (BX) and Shorthorn-Hereford (SH) lines in the years 1960–8. Fertility was based on the number of calves born, whether alive or dead, to the number of cows mated.

In the foundation cows, Hereford cows had a fertility 9% higher than the Shorthorns. The difference between the bull breeds was not significant although the mean for the Brahman bulls was 16% below the mean of the other three breeds. There was a large variation in the fertility of the Brahman bulls. The fertility of the Shorthorn cows was depressed (by 8%) in the lactating cows as compared with dry cows, while there was an opposite effect in the Herefords. These latter effects showed year to year variation.

In the F1 generation the differences between the breeds were not significant although both the AX (76·4%) and the BX (81·2%) were more fertile than the SH (70·1%). Estimates of heterosis in the F1 generation were 42% for the AX, 43% for the BX and 12% for the SH. Lactating cows were 7% more fertile than non-lactating cows. There were significant differences between the BX bulls used but not between bulls of the other two breeds. The effect of sires within breed on fertility of daughters was significant only within the SH, and the heritabilities of fortuity were estimated from the variance components for sires within breed to be 9%, 14% and 22% for AX, BX and SH respectively.

In the F2 and F3 data the breeds were significantly different in fertility with averages of 77%, 61% and 67% for AX, BX and SH respectively. Thus by comparison with the fertilities of the F1 cows no loss of heterosis for fertility occurred in the AX, a very marked loss in the BX and only a slight loss in the SH. A significant interaction between age of cow and lactational status showed that in the mature cows, wet cows had a higher fertility than dry, while the converse applied in the 3-year-old cows. The interaction of lactational status with breed consisted of the wet zebu cross cattle having a relatively low fertility while the wet British cattle had a relatively high fertility. There were significant differences between bulls within each of the three breeds. The effect of sires was significant in the BX and SH. Heritabilities estimated from between sires within breeds variance components were – 12%, 22% and 25% for the AX, BX and SH respectively.

An assessment was made of the possible significance of hypocotyl length in the identification of brussels-sprout varieties. The investigation was based on the analysis of 162 samples of varieties currently in commerce, a search for sibs amongst supposed F1 varieties and the comparison of known and unknown varieties. Varieties were found to vary consistently from one another, although environmental and environmental by genotype interaction effects were significant. It was found that sibs could be recognized easily in F1 hybrid samples known to contain them and it was inferred, and later evidence confirmed, that they were present in other F1 hybrids. Up to 8 out of 13 F1 varieties were correctly identified from coded samples and it was concluded that hypocotyl characteristics could be used with other measurements in the description of new introductions.

Four field experiments (1968–71) investigated the effect of changing the length of the growing period on the nitrogen fertilizer requirement of sugar beet. The crop was sown on three occasions (March–May), harvested on three occasions (September–December) and given four amounts of fertilizer (0–225 kg N/ha). Plant samples were analysed at several stages of growth (1969–71) in an attempt to predict the amount of nitrogen fertilizer needed for maximum sugar yield and also at the end of the season to determine the nitrogen uptake. Increasing the length of the growing period increased sugar yield greatly but the amount of nitrogen fertilizer needed for maximum sugar yield was unchanged. The crop given the largest dressing of nitrogen and with the longest growing period contained most total nitrogen, but in every experiment, giving more than 75 kg N/ha neither increased nor decreased the sugar yield significantly. As a result of the small variations in nitrogen requirement, the plant analyses during the growing season were of little value in predicting the needs of the crop.

Experiments made between 1967 and 1970 tested the effects of sowing oilseed rape on different dates in autumn and spring. Much greater oil yields were obtained from autumn sowings, due to higher seed yields and oil content, but the effect varied considerably between experiments, varieties and seasons. Autumn-sown crops flowered in late April or early May, and by the time pods were developing rapidly in June the leaves had senesced almost completely. Spring-sown varieties followed a similar pattern but about a month later, and did not reach the same peak in pod growth.

There was a major effect of sowing date, late-autumn sowings (after mid-September) giving lower yields than early-spring sowings. The best autumn sowings (early September) gave about 3 t/ha of seed, but late sowings less than half this; oil content also decreased with delay in sowing – from 45% to as low as 38%. Delayed sowing restricted growth, leaf area and pod production, and also had a slight effect on seed weight. Frost damage on flower buds appeared to be the cause of reduced yield from early sowing (August) in one experiment. In spring, the earliest sowing (late March) gave the best yields. Vigorous but late vegetative growth from the latest sowing (end of April) did not lead to high yields, possibly due to pest and disease effects.

In experiments at Sutton Bonington between 1967 and 1970, which tested 0–300 kg N/ha applied in spring, maximum oil yields of both autumn- and spring-sown oilseed rape were obtained by applying 200 kg N/ha. The seed-oil content was reduced by nitrogen application but only seriously when 300 kg/a was applied. Responses to high levels of potassium (180 rather than 60 kg K20/ha) were only obtained at 200 kg N/a. Successive increments of nitrogen from nil to 300 kg/ha improved plant growth, increasing leaf area and the dry weight of leaves and stems. The last increment from 200 to 300 kg N/ha was less effective and either reduced or did not affect pod production.

In one experiment 300 kg N/ha stimulated husk growth, apparently at the expense of seed growth, with 200 kg/ha giving the highest proportion of pod dry matter as seed. More nitrogen always raised number of seeds per pod, but only increased seed weight in one autumn-sown experiment.

Serial harvests of one experiment indicated that the maximum seed and oil yield was obtained by cutting plants before they were fully ripe, thus avoiding seed loss through pod shatter. The oil content of the bulk samples reached a peak about a week before maximum oil yield, probably because later-ripening seed did not reach full maturity. All nitrogen levels delayed flowering, but only the highest delayed maturity in the autumn-sown crop.

The efficiency of utilization of the energy of food by weaned pigs of the Large White breed was measured. Three diets based on maize (40–60%) had a metabolizable energy of 75·8±1·2% of the gross energy, and 78·2 ±3·4% of the metabolizable energy was present as net energy.

The maintenance requirement of metabolizable energy of pigs weighing 14·3 ± 2·1 kg was 143·6 kcal/kg°0·75/24 h (601 kJ/kg0·75/24 h).

The energy cost per g protein and fat synthesized by piglets was estimated to be 7·43 and 12·05 kcal (31·1 and 50·4 kJ) metabolizable energy, respectively.

Equations for calculation of metabolizable energy (Yl, kcal) and of the net energy (Y2, kcal), based on digested nutrients, were:

Y1 = 4·64×1 + 9·12×2 + 4·25×3'CV = ± 1·2%,

Y2 = 3·84×1 + 7·09×2 + 3·28×3'CV = ± 1·4%,

where X1 = g protein digested, X2 = g fat digested, X3= g carbohydrate digested (crude fibre × N-free extracts).

Pulverized fuel ash and fiuidized combustor ash were used to modify the availablewater capacity and moisture characteristics of a gravelly sandy loam soil in a study of the effects of fine-particle amendments on crop yield. Intimate incorporation of the ashes into the top-soil at rates of up to 502 t/ha increased the available water retained in the surface 0·3 m of soil by 40–80 %. The increase was mainly water held at potentials of between –0·05 bar and – 1 bar.

Two sugar-beet crops followed by broad beans, salad onions and cabbage were grown in the amended soil. With the highest rate of ash addition yield of the onions was increased by over 30% and of the cabbages by 14%. No improvement in sugar-beet or broad-bean yields resulted from the treatment. Poor growth of the second sugar-beet crop was attributed to a reduced aeration effect but it is suggested that the failure of the increased available water to improve yield consistently in this and a previous experiment was in part caused by rapid evaporative losses from the amended soil.

Field experiments were conducted in four provinces of Iran in which sugar-beet yield responses to added nitrogen and phosphorus fertilizers were correlated with soil test values and number of irrigations.

Although significant yield responses to fertilizer application were obtained in all four provinces, extremely few significant relationships were established between soil test values and yield response.

Average crop yield was favourably influenced by the number of irrigations applied in Fare and Khorasan, by organic carbon status in Esfahan and Khorasan and adversely affected by increased soil conductivity in Esfahan and Khorasan. These results were taken to imply an inadequate number of irrigations in Fars and Khorasan. The high calcium carbonate status found in Fars soil adversely affected the level of average yield.

Response to nitrogen fertilizer declined in Fars and Khorasan as the leaf nitrogen exceeded 3·15 and 4·0% respectively. Response to phosphate fertilizer declined in West Azerbaijan and Khorasan when leaf phosphorus exceeded 0·4%.

In this experiment an attempt was made to study the influence of breed and sex on the muscle-weight distribution of cattle. The weights of individual muscles obtained by total dissection from the side of a carcass from each of 63 bulls, 106 steers and 22 heifers representing six, eight and two breed groups respectively were classified into nine anatomical groups using the method of Butterfield (1963). Muscle-weight distribution was then studied by expressing the muscle in each of these groups as percentages of total muscle and also as adjusted mean weight of muscle in each region while statistically adjusting total muscle to a constant level.

Results indicated that breed differences were significant although small for abdominal muscles and muscles of the neck region within bulls and steers, but two breed groups of heifers did not differ. There was no detectable breed influence on the percentage of any other muscle group. Percentages of muscles classified as expensive were found to be remarkably similar among breed groups in all three sexes.

Sex influences on muscle distribution were also appraised. There was a general trend of heifers having a higher percentage in the proximal pelvic limb and abdominal areas than steers, while steers exceeded bulls. This order of sex influence was reversed in the muscles of the neck and thorax region. The influence of sex was conspicuous in areas classified as having expensive muscles, with heifers having a higher percentage of muscles in the high-priced regions than steers and steers being superior to bulls. Sex differences reflect the differential development of bulls compared with the other sexes as they mature. Muscles of the neck and thorax in bulls increase in proportion and other groups (proximal hind and abdominal) decrease. The differentiation of muscles represents a trend toward masculinity from heifer to steer to young bull and finally to old bull proportions.

Procedures for measuring K+ and NO-3 activities in the root zones of field crops, using specific-ion electrodes, were standardized. For K, a 1·0 M-NaCl salt bridge and KC1 standards in water, for NO3, a saturated KC1 salt bridge and KN03 standards in water, and for both electrodes, a 1:0·5, soil: water ratio, and 30 sec equilibration time were found satisfactory.

Recovery of added K in soil pastes by the K electrode and chemical analysis of the soil water extract compared well, but the recovery was about 8% only. The corresponding recovery of added N was about 87 and 95% respectively.

Relative changes in the rates and magnitudes of NO3 and K concentrations were measured with these electrodes, laterally and vertically, in the root zone, during active crop growth, from the N2 ½(PKNaMg), N2 PKNaMg, and N4PKNaMg treatments of the Broadbalk Winter Wheat Experiment.

In all fertilizer treatments, at all times, the nutrient concentrations were most at 45 cm from the crop (in the uncropped area) and least within the cropped area. The differences between these extremes represent nutrient depletion by the crop, the ‘45 cm’ measurementsindicating changes in uncropped, but fertilized, areas.

Soil nitrate depletion by the crop was much more at 12·5 cm and 20 cm depths than at 5 cm. Maximum NO3 depletion was observed during the later stages of crop growth, at ‘pre-panicle emergence’ and at ‘grain filling’. Depletion decreased and the soil NO3 level recovered partially as the crop reached maturity.

Periodic changes in the K concentration at each site and the corresponding K depletions were much less. Periods of IC stress on the soil were few and less clearly demarcated. Soil K concentration started to recover at the ‘grain filling’ stage about a month earlier than with NO3.

Changes in NO3 and K concentrations seem to relate more to the amounts given of each nutrient, than to the N:K ratio in each fertilizer treatment. However, changes in NO3 and K concentrations, and also NO3 and K depletion, occurred consecutively. This indicates an alternating periodicity in the demands of the crop for NO3 and K respectively throughout growth.

An experiment at Sutton Bonington in 1971 tested the effects of sowing and harvesting dates on yield of sugar beet. Seed sown on 28 March germinated slowly in the cold soil and crop establishment was poor. From early April until the beginning of May the loss in yield of sugar consequent upon delaying sowing by 1 week was 0·4 t/ha. For later sowings the yield loss was greater. During the harvest period the rate of increase in sugar yield was the same for all sowings. Yields of crops sown and harvested on different dates were closely correlated withthe amount of solar radiation intercepted by the leaf canopy.

The production rates of bacteria have been measured in the rumen of zebu calves and buffalo calves. The animals were fed green oat continuously at 2 h intervals. [35S]sodium sulphate was fed to the animals for 5 days at 2 h intervals by mixing in the feed. On the sixth day the radioactive feed was stopped and replaced by non-radioactive feed. The decline in the specific radioactivity of bacterial cells in the rumen as a function of time was taken for calculating t/2. Simultaneously rumen volume was determined and pool size of bacterial cells was calculated in the rumen. A mathematical equation was applied to calculate the production rates of bacteria. The production rates of bacteria, on average, were 100·5 and 72·3 mg/min for buffalo calves and zebu calves, respectively.

Studies were made of particle size distribution in meals produced from typical ingredients used in pig diets and of dietaries formulated from such ingredients. Each meal was subjected to a sieving procedure through standard sieves and the fractions retained by the sieves were weighed. The modes of expressing the results included moduli of fineness and uniformity and cumulative totals of oversize material, the latter forming the basis of the system preferred. Preliminary investigations into factors of loading weight, time of shaking and reproducibility of results led to the ultimate adoption of a standard technique of mechanical shaking of 100 g loads for 10 min. The technique was used to study particle size distribution in various cereals, cereal by-products, animal and vegetable protein sources and mineral mixtures employed in pig dietary formulations. The studies also included the particle distribution pattern in barley milled at varying grist sizes and in replicated samples of a commercial pig meal.

The suggested technique of characterizing particle size distribution should materially assist investigations into problems of pig health and productivity that appear to be associated with grist size of dietary meals.