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Whey protein concentrate improves antioxidant capacity, faecal microbiota and fatty acid profile of growing piglets

Published online by Cambridge University Press:  09 May 2019

I. Kafantaris ,
D. Stagos ,
B. Kotsampasi ,
D. Kantas ,
V. Koukoumis ,
N. D. Moschonas ,
Z. Skaperda ,
K. Gerasopoulos ,
S. Makri  and
N. Goutzourelas
...Show all authors
I. Kafantaris
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece Animal Research Institute/Hellenic Agricultural Organization - Demeter, 58100 Giannitsa, Greece
D. Stagos
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
B. Kotsampasi
Affiliation:
Animal Research Institute/Hellenic Agricultural Organization - Demeter, 58100 Giannitsa, Greece
D. Kantas
Affiliation:
Department of Animal Production, Technical Education Institute of Thessaly, 41110 Larissa, Greece
V. Koukoumis
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
N. D. Moschonas
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
Z. Skaperda
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
K. Gerasopoulos
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
S. Makri
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
N. Goutzourelas
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
C. Mitsagga
Affiliation:
Department of Food Technology, Lab of Food Microbiology and Biotechnology, Technical Education Institute of Thessaly, 43100 Karditsa, Greece
I. Giavasis
Affiliation:
Department of Food Technology, Lab of Food Microbiology and Biotechnology, Technical Education Institute of Thessaly, 43100 Karditsa, Greece
K. Petrotos
Affiliation:
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece
S. Kokkas
Affiliation:
Department of Biosystem Engineering, Technical Education Institute of Thessaly, 41110 Larissa, Greece
D. Kouretas*
Affiliation:
Department of Biochemistry and Biotechnology, University of Thessaly, Viopolis, Mezourlo, 41500 Larissa, Greece
*
Author for correspondence: D. Kouretas, E-mail: [email protected]
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Abstract

A feeding trial involving growing piglets was undertaken to establish whether feed supplemented with whey protein concentrate (WPC), exhibiting antioxidant properties, had any effects on welfare and meat quality. For that purpose, 48 weaned piglets (20-days-old) were assigned to two experimental groups receiving standard or experimental diet for 30 days. Blood and tissue collection were performed at various time-points. The following oxidative stress markers were assessed: reduced glutathione (GSH), catalase activity, total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB) and hydrogen peroxide (H2O2) decomposition activity. The effects on bacterial growth and the fatty acid profile of meat were also assessed. Results showed that piglets fed with the WPC-supplemented diet had significantly increased antioxidant mechanisms in almost all tissues tested, as indicated by increases in GSH, H2O2 decomposition activity and TAC compared with the control group. Piglets fed with the experimental diet exhibited decreased oxidative stress-induced damage to lipids and proteins, as shown by decreases in TBARS and CARB in the WPC group compared with the control group. In addition, the experimental diet enhanced growth of facultative probiotic bacteria and lactic acid bacteria and inhibited growth of pathogen populations. In addition, WPC inclusion in piglets' diet increased n-3 fatty acids significantly and decreased n-6/n-3 ratio significantly compared with the control group. The current study showed that WPC inclusion in the diet had a significant effect on welfare and meat quality of growing piglets.

Keywords

Fatty acidsfaecal microbiotaoxidative stresspigswhey protein
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 1 , January 2019 , pp. 72 - 82
Copyright
Copyright © Cambridge University Press 2019 

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References

Aebi, H (1984) Catalase in vitro. Methods in Enzymology 105, 121–126.Google Scholar
Aquilano, K, Baldelli, S and Ciriolo, MR (2014) Glutathione: new roles in redox signaling for an old antioxidant. Frontiers in Pharmacology 26, article no. 196, 1–12. doi: 10.3389/fphar.2014.00196.Google Scholar
Chen, YP, Chen, X, Zhang, H and Zhou, YM (2013) Effects of dietary concentrations of methionine on growth performance and oxidative status of broiler chickens with different hatching weight. British Poultry Science 54, 531–537.Google Scholar
Chierici, R, Fanaro, S, Saccomandi, D and Vigi, V (2003) Advances in the modulation of the microbial ecology of the gut in early infancy. Acta Paediatrica 92, 56–63.Google Scholar
DeLeve, LD and Kaplowitz, N (1990) Importance and regulation of hepatic glutathione. Seminars in Liver Disease 10, 251–266.Google Scholar
Field, CJ, Blewett, HH, Proctor, S and Vine, D (2009) Human health benefits of vaccenic acid. Applied Physiology, Nutrition, and Metabolism 34, 979–991.Google Scholar
Gerasopoulos, K, Stagos, D, Kokkas, S, Petrotos, K, Kantas, D, Goulas, P and Kouretas, D (2015 a) Feed supplemented with byproducts from olive oil mill wastewater processing increases antioxidant capacity in broiler chickens. Food and Chemical Toxicology 82, 42–49.Google Scholar
Gerasopoulos, K, Stagos, D, Petrotos, K, Kokkas, S, Kantas, D, Goulas, P and Kouretas, D (2015 b) Feed supplemented with polyphenolic byproduct from olive mill wastewater processing improves the redox status in blood and tissues of piglets. Food and Chemical Toxicology 86, 319–327.Google Scholar
Gerasopoulos, K, Stagos, D, Krouezas, A, Karaveli, C, Barda, C, Gkika, H, Mitsiou, D, Petrotos, K, Goulas, P and Kouretas, D (2016) Assessment of fatty acid allocation in plasma and tissues in piglets, using feed supplemented with byproducts from processed olive mill wastewater. In Vivo 30, 291–301.Google Scholar
Giang, HH, Viet, TQ, Ogle, B and Lindberg, JE (2010) Growth performance, digestibility, gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria. Livestock Science 129, 95–103.Google Scholar
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KVV and Bauman, DE (2000) Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9-desaturase. Journal of Nutrition 130, 2285–2291.Google Scholar
Haraguchi, FK, Silva, ME, Neves, LX, dos Santos, RC and Pedrosa, ML (2011) Whey protein precludes lipid and protein oxidation and improves body weight gain in resistance- exercised rats. European Journal of Nutrition 50, 331–339.Google Scholar
Ibrahim, SA and Bezkorovainy, A (1993) Survival of bifidobacteria in the presence of bile salt. Journal of the Science of Food and Agriculture 62, 351–354.Google Scholar
Janaszewska, A and Bartosz, G (2002) Assay of total antioxidant capacity: comparison of four methods as applied to human blood plasma. Scandinavian Journal of Clinical and Laboratory Investigation 62, 231–236.Google Scholar
Kafantaris, I, Kotsampasi, B, Christodoulou, V, Kokka, E, Kouka, P, Terzopoulou, Z, Gerasopoulos, K, Stagos, D, Mitsagga, C, Giavasis, I, Makri, S, Petrotos, K and Kouretas, D (2017) Grape pomace improves antioxidant capacity and faecal microflora of lambs. Journal of Animal Physiology and Animal Nutrition 101, e108–e121.Google Scholar
Kafantaris, I, Stagos, D, Kotsampasi, B, Hatzis, A, Kypriotakis, A, Gerasopoulos, K, Makri, S, Goutzourelas, N, Mitsagga, C, Giavasis, I, Petrotos, K, Kokkas, S, Goulas, P, Christodoulou, V and Kouretas, D (2018) Grape pomace improves performance, antioxidant status, fecal microbiota and meat quality of piglets. Animal: An International Journal of Animal Bioscience 12, 246–255.Google Scholar
Kailasapathy, K and Chin, J (2000) Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp. Immunology and Cell Biology 78, 80–88.Google Scholar
Keles, MS, Taysi, S, Sen, N, Aksoy, H and Akçay, F (2001) Effect of corticosteroid therapy on serum and CSF malondialdehyde and antioxidant proteins in multiple sclerosis. Canadian Journal of Neurological Sciences 28, 141–143.Google Scholar
Kerasioti, E, Kiskini, A, Veskoukis, A, Jamurtas, A, Tsitsimpikou, C, Tsatsakis, AM, Koutedakis, Y, Stagos, D, Kouretas, D and Karathanos, V (2012) Effect of a special carbohydrate-protein cake on oxidative stress markers after exhaustive cycling in humans. Food and Chemical Toxicology 50, 2805–2810.Google Scholar
Kerasioti, E, Stagos, D, Jamurtas, A, Kiskini, A, Koutedakis, Y, Goutzourelas, N, Pournaras, S, Tsatsakis, AM and Kouretas, D (2013) Anti-inflammatory effects of a special carbohydrate-whey protein cake after exhaustive cycling in humans. Food and Chemical Toxicology 61, 42–46.Google Scholar
Kerasioti, E, Stagos, D, Priftis, A, Aivazidis, S, Tsatsakis, AM, Hayes, AW and Kouretas, D (2014) Antioxidant effects of whey protein on muscle C2C12 cells. Food Chemistry 155, 271–278.Google Scholar
Kerasioti, E, Stagos, D, Georgatzi, V, Bregou, E, Priftis, A, Kafantaris, I and Kouretas, D (2016 a) Antioxidant effects of sheep whey protein on endothelial cells. Oxidative Medicine and Cellular Longevity 2016, e6585737. http://dx.doi.org/10.1155/2016/6585737.Google Scholar
Kerasioti, E, Stagos, D, Tzimi, A and Kouretas, D (2016 b) Increase in antioxidant activity by sheep/goat whey protein through nuclear factor-like 2 (Nrf2) is cell type dependent. Food and Chemical Toxicology 97, 47–56.Google Scholar
Kerasioti, E, Terzopoulou, Z, Komini, O, Kafantaris, I, Makri, S, Stagos, D, Gerasopoulos, K, Anisimov, NY, Tsatsakis, AM and Kouretas, D (2017) Tissue specific effects of feeds supplemented with grape pomace or olive oil mill wastewater on detoxification enzymes in sheep. Toxicology Reports 4, 364–372.Google Scholar
Kerasioti, E, Stagos, D, Tsatsakis, AM, Spandidos, DA, Taitzoglou, I and Kouretas, D (2018) Effects of sheep/goat whey protein dietary supplementation on the redox status of rats. Molecular Medicine Reports 17, 5774–5781.Google Scholar
Klewicki, R and Klewicka, E (2004) Antagonistic activity of lactic acid bacteria as probiotics against selected bacteria of the Enterobaceriacae family in the presence of polyols and their galactosyl derivatives. Biotechnology Letters 26, 317–320.Google Scholar
Kotsampasi, B, Christodoulou, V, Zotos, A, Liakopoulou-Kyriakides, M, Goulas, P, Petrotos, K, Natas, P and Bampidis, VA (2014) Effects of dietary pomegranate byproduct silage supplementation on performance, carcass characteristics and meat quality of growing lambs. Animal Feed Science and Technology 197, 92–102.Google Scholar
Liu, Y, Hyde, AS, Simpson, MA and Barycki, JJ (2014) Emerging regulatory paradigms in glutathione metabolism. Advances in Cancer Research 122, 69–101.Google Scholar
Makri, S, Kafantaris, I, Stagos, D, Chamokeridou, T, Petrotos, K, Gerasopoulos, K, Mpesios, A, Goutzourelas, N, Kokkas, S, Goulas, P, Komiotis, D and Kouretas, D (2017) Novel feed including bioactive compounds from winery wastes improved broilers' redox status in blood and tissues of vital organs. Food and Chemical Toxicology 102, 24–31.Google Scholar
Patsoukis, N, Zervoudakis, G, Panagopoulos, NT, Georgiou, CD, Angelatou, F and Matsokis, NA (2004) Thiol redox state (TRS) and oxidative stress in the mouse hippocampus after pentylenetetrazol-induced epileptic seizure. Neuroscience Letters 357, 83–86.Google Scholar
Pereira, DI and Gibson, GR (2002) Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. Applied and Environmental Microbiology 68, 4689–4693.Google Scholar
Reddy, YN, Murthy, SV, Krishna, DR and Prabhakar, MC (2004) Role of free radicals and antioxidants in tuberculosis patients. Indian Journal of Tuberculosis 51, 213–218.Google Scholar
Ritvanen, T, Putkonen, T and Peltonen, K (2012) A comparative study of the fatty acid composition of dairy products and margarines with reduced or substituted fat content. Food and Nutrition Sciences 3, 1189–1196.Google Scholar
Ross, GR, Van Nieuwenhove, CP and González, SN (2012) Fatty acid profile of pig meat after probiotic administration. Journal of Agricultural and Food Chemistry 60, 5974–5978.Google Scholar
Santini, C, Baffoni, L, Gaggia, F, Granata, M, Gasbarri, R, Di Gioia, D and Biavati, B (2010) Characterization of probiotic strains: an application as feed additives in poultry against. Campylobacter jejuni. International Journal of Food Microbiology 141(Suppl. 1), S98–S108.Google Scholar
Sauvant, D, Perez, JM and Tran, G (2004) Tables of Composition and Nutritional Value of Feed Materials: Pigs, Poultry, Cattle, Sheep, Goats, Rabbits, Horses and Fish, INRA Editions and AFZ. Wageningen, the Netherlands: Wageningen Academic Publishers.Google Scholar
Simopoulos, AP (2002) Omega-3 fatty acids in inflammation an autoimmune diseases. Journal of the American College of Nutrition 21, 495–505.Google Scholar
Walzem, RL, Dillard, CJ and German, JB (2002) Whey components: millennia of evolution create functionalities for mammalian nutrition: what we know and what we may be overlooking. Critical Reviews in Food Science and Nutrition 42, 353–375.Google Scholar
Wang, X, Niu, C, Lu, J, Li, N and Li, J (2014) Hydrolyzed protein supplementation improves protein content and peroxidation of skeletal muscle by adjusting the plasma amino acid spectrums in rats after exhaustive swimming exercise: a pilot study. Journal of the International Society of Sports Nutrition 11, article no. 5, 1–6. doi: 10.1186/1550-2783-11-5.Google Scholar
Xu, R, Liu, N, Xu, X and Kong, B (2011) Antioxidative effects of whey protein on peroxide induced cytotoxicity. Journal of Dairy Science 94, 3739–3746.Google Scholar
Zhu, LH, Zhao, KL, Chen, XL and Xu, JX (2012) Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs. Journal of Animal Science 90, 2581–2589.Google Scholar