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Veno-arterial differences of immunoreactive and biologically active luteinizing hormone across the head in the cow

Published online by Cambridge University Press:  27 March 2009

R. J. van Aarde ,
R. B. Heap ,
M. Hamon  and
I. R. Fleet
R. J. van Aarde
Affiliation:
Mammal Research InstituteUniversity of PretoriaPretoria 0002South Africa
R. B. Heap
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
M. Hamon
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
I. R. Fleet
Affiliation:
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4 AT, UK
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Summary

Immunoreactive (I) and biologically active (B) luteinizing hormone (LH) was measured in jugular vein and carotid arterial plasma of four cows 110–140 min after luteinizing hormone releasing hormone (LHRH) injection. Plasma was separated on Ultragel AcA54 and fractions corresponding to those containing authentic bovine luteinizing hormone (bLH) were assayed. Immunoreactivity in venous or arterial plasma occurred mostly in fractions corresponding in molecular weight to bLH. Low activity was measured in several other fractions. Biological activity was also high in the same fractions from venous plasma, though it was not so pronounced in arterial plasma.

A veno-arterial difference across the head for immunoreactive LH was found in all animals, and there was a significant difference in biologically active LH (mouse Leydig cell assay). The mean B:I value for immunoreactive LH was similar in venous and arterial plasma for three out of four animals, 0·72 and 0·66, respectively. In a fourth cow with a clinical history of infertility, the B:I value was low in both venous and arterial plasma, 0·19 and 0·08, respectively. The results indicate that infertility in this latter instance was associated with LHRH-induced secretion of a compound similar in molecular size and immunoreactivity to LH but deficient in biological potency.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 113 , Issue 1 , August 1989 , pp. 27 - 33
Copyright
Copyright © Cambridge University Press 1989

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References

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