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Transfer of transgenes for resistance to rice tungro disease into high-yielding rice cultivars through gene-based marker-assisted selection

Published online by Cambridge University Press:  26 October 2012

S. ROY ,
A. BANERJEE ,
J. TARAFDAR ,
B. K. SENAPATI  and
I. DASGUPTA
S. ROY
Affiliation:
Department of Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, India
A. BANERJEE
Affiliation:
Department of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal 741252, India
J. TARAFDAR
Affiliation:
Department of Plant Pathology, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal 741252, India
B. K. SENAPATI
Affiliation:
Department of Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, India
I. DASGUPTA*
Affiliation:
Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi 110021, India
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Rice tungro disease (RTD), caused by the simultaneous infection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV), is one of the major threats to sustainable rice production in South and Southeast Asia. Transgenic resistance against RTBV has been reported previously using an RNA interference (RNAi) construct (ORF IV of RTBV, placed both in sense and anti-sense orientation under CaMV 35S promoter), in the scented rice line Pusa Basmati-1 (PB-1). This construct was transferred to two high-yielding tungro-susceptible indica rice cultivars (IET4094 and IET4786) from the transgenic PB-1 rice line using back cross breeding till the BC2F3 stage. On challenge inoculation, the progenies (BC2F1) showed mild symptoms of tungro, in contrast to severe symptoms displayed by the recurrent parents. Segregation of the transgene indicated near homozygosity of the plants at the BC2F3 stage, implying that the lines can be used as a valuable resistance source for further breeding against RTD.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 150 , Issue 5 , October 2012 , pp. 610 - 618
Copyright
Copyright © Cambridge University Press 2011

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