Published online by Cambridge University Press: 27 March 2009
The extent of the change in pH on incubation, when bull sperm were suspended in various fluids of different composition, was affected by the buffer capacity, the pH, and the glucose content. In one such fluid, used to replace seminal fluid, there was a highly significant positive correlation between the pH. change and the number and motility of the sperm, and between the pH change in this fluid and in undiluted semen. The various conditions which appear to affect the increase in acidity are discussed, and it is concluded that there is no practical object in determining the change in pH on incubation in an artificial fluid of uniform and constant composition, instead of in semen.