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Soil adaptation of transgenic in vitro carrot plantlets

Published online by Cambridge University Press:  08 September 2008

H. MIKSCHOFSKY ,
G. MANN  and
I. BROER
H. MIKSCHOFSKY*
Affiliation:
Agricultural and Environmental Faculty, Agrobiotechnology, University of Rostock, Justus-von-Liebig-Weg 8, 18059, Germany
G. MANN
Affiliation:
Agricultural and Environmental Faculty, Agrobiotechnology, University of Rostock, Justus-von-Liebig-Weg 8, 18059, Germany
I. BROER
Affiliation:
Agricultural and Environmental Faculty, Agrobiotechnology, University of Rostock, Justus-von-Liebig-Weg 8, 18059, Germany
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Adapting in vitro transgenic carrots to soil is the most crucial step preceding the field investigation of transgenic carrots. A low proportion of plants, around 0·20, acclimatize to soil (Hardegger & Sturm 1998) and thus prohibit the generation of high-expression carrot lines. In the present paper, a protocol for an efficient soil transfer is presented and the impact of carrot cultivar, soil substrate, tissue culture, and transformation process on transfer process is analysed. Somatic embryo germinants of Daucus carota cv. Rote Riesen 2 and Lobbericher Gelbe Futtermoehre showed a tremendous survival proportion – up to 1·00 – when transferred to their optimal soil substrate: sandy and loamy soil, with low content of macro and micro elements and a pH of 5·8. By optimizing the conditions described here, the proportion of soil acclimatized transgenic carrot plants of D. carota Lobbericher Gelbe Futtermoehre was increased from 0·1 to 0·87, and for the cultivar Rote Riesen from 0·09 to 0·67.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 147 , Issue 1 , February 2009 , pp. 43 - 49
Copyright
Copyright © 2008 Cambridge University Press

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