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Screening of plant extracts for antifungal activities against Colletotrichum species of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp)

Published online by Cambridge University Press:  17 July 2012

J. I. G. MASANGWA ,
T. A. S. AVELING  and
Q. KRITZINGER
J. I. G. MASANGWA
Affiliation:
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa
T. A. S. AVELING*
Affiliation:
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa
Q. KRITZINGER
Affiliation:
Department of Plant Science, University of Pretoria, Pretoria 0002, South Africa
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

The aim of the present investigation was to evaluate the antifungal activities of plant extracts which can be used to control bean and cowpea anthracnose. Acetone, ethyl acetate and water extracts of Ipomoea batatas, Carica papaya, Allium sativum, Syzygium cordatum, Chlorophytum comosum and Agapanthus caulescens were screened in vitro for their antifungal activities against Colletotrichum lindemuthianum and Colletotrichum dematium of common bean and cowpea using the agar disc infusion and microtitre double-dilution techniques. The same extracts were then tested for antifungal activity in vivo as seed treatments against anthracnose disease. The water extracts of Carica and Syzygium were active against C. lindemuthianum and had minimum inhibitory concentrations (MICs) of 1·56 mg/ml. Syzygium, Allium and Chlorophytum water extracts were active against C. dematium and MICs were 3·13, 6·25 and 12·5 mg/ml, respectively. The MICs of Allium, Syzygium and Agapanthus acetone extracts were 0·78, 3·13 and 6·25 mg/ml, respectively, against C. lindemuthianum and 0·78, 6·25 and 3·13 mg/ml against C. dematium. Agapanthus water extracts and all the acetone extracts tested in vivo effectively reduced the incidence and severity of bean anthracnose disease in the greenhouse. Agapanthus acetone, Allium water, and both acetone and water extracts of Carica and Syzygium performed well in vivo in reducing cowpea anthracnose disease and compared well with reductions due to the application of the synthetic fungicide fludioxonil+mefenoxam (the commercial product Celest® XL) applied at 25 gai/l and also with levels in the non-inoculated control. The Agapanthus, Carica, Syzygium and Allium extracts were active on both Colletotrichum spp. in vitro and also reduced anthracnose disease of bean and cowpea and are potential seed treatments in anthracnose disease control. The easy seed treatment process and the accessibility of plants used in the present study could lead to high adoption of the use of the plant extracts as seed treatments by resource-poor, smallholder farmers.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 151 , Issue 4 , August 2013 , pp. 482 - 491
Copyright
Copyright © Cambridge University Press 2012 

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References

REFERENCES

Adebanjo, A. & Bankole, S. A. (2004). Evaluation of some fungi and bacteria for biocontrol of anthracnose disease of cowpea. Journal of Basic Microbiology 44, 3–9.CrossRefGoogle ScholarPubMed
Akinbode, O. A. & Ikotun, T. (2008). Evaluation of some bioagents and botanicals in in vitro control of Colletotrichum destructivum. African Journal of Biotechnology 7, 868–872.Google Scholar
Allen, D. J. (1983). The Pathology of Tropical Food Legumes: Disease Resistance in Crop Improvement. Chichester, UK: John Wiley and Sons.Google Scholar
Amadioha, A. C. (2003). Evaluation of some plant leaf extracts against Colletotrichum lindemuthianum in cowpea. Acta Phytopathologica et Entomologica Hungarica 38, 259–265.CrossRefGoogle Scholar
Anibijuwon, I. I. & Udeze, A. O. (2009). Antimicrobial activity of Carica papaya (pawpaw leaf) on some pathogenic organisms of clinical origin from South-Western Nigeria. Ethnobotanical Leaflets 13, 850–864.Google Scholar
Aveling, T. A. S., Govender, V., Kandolo, D. S. & Kritzinger, Q. (2012). The effects of treatments with selected pesticides on viability and vigour of maize (Zea mays) seeds and seedling emergence in the presence of Fusarium graminearum. Journal of Agricultural Science, Cambridge, published online doi:10.1017/S0021859612000457.Google Scholar
Beaver, J. S., Rosas, J. C., Myers, J., Acosta, J., Kelly, J. D., Nchimbi-Msolla, S., Misangu, R., Bokosi, J., Temple, S., Arnaud-Santana, E. & Coyne, D. P. (2003). Contributions of the bean/cowpea CRSP to the management of bean diseases. Field Crops Research 82, 87–102.CrossRefGoogle Scholar
Cano, J., Guarro, J. & Gené, J. (2004). Molecular and morphological identification of Colletotrichum species of clinical interest. Journal of Clinical Microbiology 42, 2450–2454.CrossRefGoogle ScholarPubMed
Cao, K. Q. & Van Bruggen, A. H. C. (2001). Inhibitory efficacy of several plant extracts and plant products on Phytophthora infestans. Journal of the Agricultural University of Hebei 2, 90–96.Google Scholar
Centro Internacional De Agricultura Tropical (CIAT) (1987). Standard System for the Evaluation of Bean Germplasm (Eds Van Schoonhoven, A. & Pastor-Corrales, M. A.). Cali, Colombia: CIAT.Google Scholar
Chowdhury, M. N. A., Rahim, M. A., Khalequzzaman, K. M., Humauan, M. R. & Alam, M. M. (2007). Effect of plant extracts and time of application on incidence of anthracnose, yield and quality of mango. International Journal of Sustainable Crop Production 2, 59–68.Google Scholar
Falandysz, J. (2000). Residues of hexachlorobenzene in Baltic fish and estimation of daily intake of this compound and pentachlorobenzene with fish and fishery products in Poland. Polish Journal of Environmental Studies 9, 377–383.Google Scholar
Fawole, O. A., Finnie, J. F. & Van Staden, J. (2009). Antimicrobial activity and mutagenic effects of twelve traditional medicinal plants used to treat ailments related to the gastro-intestinal tract in South Africa. South African Journal of Botany 75, 356–362.CrossRefGoogle Scholar
Jeng, T. L., Shih, Y. J., Lai, C. C., Wu, M. T. & Sung, J. M. (2009). Anti-oxidative characterisation of NaN3-induced common bean mutants. Food Chemistry 119, 1006–1011.CrossRefGoogle Scholar
Kritzinger, Q., Lall, N. & Aveling, T. A. S. (2005). Antimicrobial activity of cowpea (Vigna unguiculata) leaf extracts. South African Journal of Botany 71, 45–48.CrossRefGoogle Scholar
Masoko, P., Picard, J. & Eloff, J. N. (2005). Antifungal activities of six South African Terminalia species (Combretaceae). Journal of Ethnopharmacology 99, 301–308.CrossRefGoogle ScholarPubMed
Mathur, S. B. & Kongsdal, O. (2003). Common Laboratory Seed Health Testing Methods for Detecting Fungi. 1st edn. Bassersdorf, CH, Switzerland: International Seed Testing Association.Google Scholar
Mdee, L. K., Masoko, P. & Ellof, J. N. (2009). The activity of extracts of seven common invasive plant species on fungal phytopathogens. South African Journal of Botany 75, 375–379.CrossRefGoogle Scholar
Mendiratta, D. K., Thamke, D., Shukla, A. K. & Narang, P. (2005). Keratitis due to Colletotrichum dematium – a case study report. Indian Journal of Medical Microbiology 23, 56–58.Google Scholar
Morris, P. F. & Ward, E. W. B. (1992). Chemoattraction of zoospores of the soybean pathogen Phytophthora sojae by isoflavones. Physiological and Molecular Plant Pathology 40, 17–22.CrossRefGoogle Scholar
Msuku, W. A. B., Saka, V. W. & Munthali, D. C. (2000). Major Diseases and Insect Pests of Beans (Phaseolus vulgaris) in Malawi: Problems and their Control. Study Guide. Lilongwe, Malawi: Bunda College of Agriculture, University of Malawi.Google Scholar
Mwine, J., Van Damme, P., Kamoga, G., Kudamba, Nasuuna M. & Jumba, F. (2011). Ethnobotanical survey of pesticidal plants used in South Uganda: Case study of Masaka district. Journal of Medicinal Plants Research 5, 1155–1163.Google Scholar
Nduagu, C., Ekefan, E. J. & Nkwankiti, A. O. (2008). Effect of some crude plant extracts on growth of Colletotrichum capsici (Synd) Butler & Bisby, causal agent of pepper anthracnose. Journal of Applied Biosciences 6, 184–190.Google Scholar
Ng, T. B. (2004). Antifungal proteins and peptides of leguminous and non-leguminous origins. Peptides 25, 1215–1222.CrossRefGoogle ScholarPubMed
Ngowi, A. V. F., Mbise, T. J., Ijani, A. S. M., London, L. & Ajayi, O. C. (2007). Smallholder vegetable farmers in Northern Tanzania: pesticides use practices, perceptions, cost and health effects. Crop Protection 26, 1617–1624.CrossRefGoogle Scholar
Pastor-Corrales, M. A. & Tu, J. C. (1989). Anthracnose. In Bean Production Problems in the Tropics (Eds Schwartz, H. F. & Pastor-Corrales, M. A.), pp. 77–104. Cali, Colombia: CIAT.Google Scholar
Pretorius, J. C., Zietsman, P. C. & Eksteen, D. (2002). Fungitoxic properties of selected South African plant species against plant pathogens of economic importance in agriculture. Annals of Applied Biology 141, 117–124.CrossRefGoogle Scholar
Raghavendra, V. B., Lokesh, S., Girisha, S. T., Govindappa, M. & Prakash, H. S. (2006). Antifungal activity of aqueous extract of safed musli against seed mycoflora of some crop species. American-Eurasian Journal of Agricultural and Environmental Sciences 1, 86–90.Google Scholar
Shovan, L. R., Bhuiyan, M. K. A., Begum, J. A. & Pervez, Z. (2008). In vitro control of Colletotrichum dematium causing anthracnose of soybean by fungicides, plant extracts and Trichoderma harzianum. International Journal of Sustainable Crop Production 3, 10–17.Google Scholar
Sibanda, T., Dobson, H. M., Cooper, J. F., Manyangarirwa, W. & Chiimba, W. (2000). Pest management challenges for smallholder vegetable farmers in Zimbabwe. Crop Protection 19, 807–815.CrossRefGoogle Scholar
Taiga, A., Suleiman, M. N., Sule, W. & Olufolaji, D. B. (2008). Comparative in vitro inhibitory effects of cold extracts of some fungicidal plants on Fusarium oxysporium Mycelium. African Journal of Biotechnology 7, 3306–3308.Google Scholar
Tegegne, G., Pretorius, J. C. & Swart, W. J. (2008). Antifungal properties of Agapanthus africanus L. extracts against plant pathogens. Crop Protection 27, 1052–1060.CrossRefGoogle Scholar
Tian, F., Zhu, J., Sun, M., Jiang, J., Wang, S. & Zhang, W. (2007). Induction and mechanism of cucumber resistance to anthracnose induced by Pieris rapae extract. Frontiers of Agriculture in China 2, 137–140.CrossRefGoogle Scholar
Vsn International (VSNi) (2008). GenStat Discovery, edition 3. Software (now ed. 4) available online at http://www.vsni.co.uk (verified 3 May 2012).Google Scholar
Wong, J. H., Zhang, X. Q., Wang, H. X. & Ng, T. B. (2006). A mitogenic defensin from white cloud beans (Phaseolus vulgaris). Peptides 27, 2075–2081.CrossRefGoogle ScholarPubMed