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Saline–alkaline resistance analysis of rice overexpressing the CsCYP1A gene of alkaline Chlorella

Published online by Cambridge University Press:  27 April 2020

Jiali Liu ,
Mingliang He ,
Chenxi Liu ,
Xu Liao ,
Xiufeng Li ,
Ling Wang Open the ORCID record for Ling Wang [Opens in a new window]  and
Qingjie Guan Open the ORCID record for Qingjie Guan [Opens in a new window]
Jiali Liu
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Mingliang He
Affiliation:
Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, HarbinHeilongjiang, 150081, China University of the Chinese Academy of Sciences, Beijing100049, China
Chenxi Liu
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Xu Liao
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Xiufeng Li*
Affiliation:
Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, HarbinHeilongjiang, 150081, China University of the Chinese Academy of Sciences, Beijing100049, China
Ling Wang*
Affiliation:
College of Landscape Architecture, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
Qingjie Guan*
Affiliation:
Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Xiangfang District, Harbin, Heilongjiang150040, China
*
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn
Authors for correspondence: Ling Wang, E-mail: wanglinghlj@126.com; Qingjie Guan, E-mail: guanqingjie@nefu.edu.cn
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Abstract

Plant cyclophilin (CYP) is related to chloroplast photoprotection, redox and other growth and developmental regulatory processes and responds to stress and improves tolerance to saline–alkali stress. Besides, it exerts peptidyl-prolyl cis/trans isomerase activity to participate in protein synthesis and folding. In this study, Northern blot was used to analyse the CsCYP1A gene (a CYP gene isolated from a Chlorella species) (accession number: KY207381) of tolerant Chlorella vulgaris in soda saline–alkali soil. The result showed that the expression of the CsCYP1A gene was induced by NaHCO3, NaCl and sorbitol. Additionally, Agrobacterium tumefaciens was used to infect the callus of rice (Oryza sativa var. Longjing11) for genetic transformation. Molecular detection confirmed that transgenic seedlings overexpressing CsCYP1A were obtained by hygromycin selection. Treatment with NaCl and NaHCO3 at the five-leaf stage was performed in the seedlings, and the results showed that there were significant differences between the CsCYP1A overexpressed rice lines and non-transgenic (NT) Longjing11 in terms of plant height, fresh weight, chlorophyll content, malondialdehyde content and ascorbate peroxidase activity. The CsCYP1A overexpression rice lines had higher tolerance to NaCl and NaHCO3 than NT. The current findings indicate that CsCYP1A can enhance the tolerance of rice to saline–alkali stress possibly through its involvement in reactive oxygen scavenging pathways.

Keywords

Adversity stressChlorellacyclophilinricetolerance
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 1-2 , March 2020 , pp. 80 - 87
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Copyright
Copyright © Cambridge University Press 2020

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