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Response of lactating goats to low phosphorus intake 2. Nitrogen transfer from rumen ammonia to rumen microbes and proportion of milk protein derived from microbial amino acids

Published online by Cambridge University Press:  27 March 2009

A. Petri ,
H. Müschen ,
G. Breves ,
O. Richter  and
E. Pfeffer
A. Petri
Affiliation:
Institut für Tierernährung der Universität Bonn, F.R. Germany
H. Müschen
Affiliation:
Institut für Tierernährung der Universität Bonn, F.R. Germany
G. Breves
Affiliation:
Institut für Tierernährung der Universität Bonn, F.R. Germany
O. Richter
Affiliation:
Institut für Tierernährung der Universität Bonn, F.R. Germany
E. Pfeffer
Affiliation:
Institut für Tierernährung der Universität Bonn, F.R. Germany
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Summary

Five rumen-cannulated dairy goats were fed diets supplying 2·8 g P/day plus 1·4 g P/kg milk (adequate P) for weeks 1–6 of lactation. Two goats then continued to receive this adequate P supply while three goats were fed deficient diets supplying only 0·8 g P/day plus 0·4 g P/kg milk. Solutions containing CrEDTA and (15NH4)2SO4 were infused into the rumen continuously for 96 h during weeks 5–6 and 11–12, respectively. At intervals following the intraruminal infusion, 15N enrichments of rumen ammonia-N, rumen bacteria-N and milk protein-N were determined. Cr concentration was measured in particle-free rumen fluid.

P deficiency did not significantly affect rumen fluid kinetics. It caused a significant increase in pH and reduced the size of the rumen ammonia pool and its outflow rate. Digestibility of organic matter as well as efficiency of microbial protein synthesis were decreased significantly and thus net microbial yield was reduced from 34·1 to 13·7 g N/day. The transfer of N of microbial origin to milk protein decreased from 5·3 to 2·7 g/day, whereas secretion of N in milk protein not originating from rumen microbes remained unchanged at 5·6–5·8 g/day.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 111 , Issue 2 , October 1988 , pp. 265 - 271
Copyright
Copyright © Cambridge University Press 1988

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References

Binnerts, W.T., Van't Klooster, A.T. & Frens, A.M. (1968). Soluble chromium indicator measured by atomic absorption in digestion experiments. Veterinary Record 82, 470.Google Scholar
Brandt, M. (1979). Versuche zur Quantifizierung der mikrobiellen Proteinsynthese im Pansen (mit Hilfe von 15N) bei Verwendung harnstoffhaltiger Rationen. Ph.D. thesis, University of Kiel.Google Scholar
Breves, G. (1985). Phosphor- und Calciumumsatz sowie Flüssigkeits- passage und mikrobielle Verdauungsvorgänge im Gastrointestinaltrakt von Schafen in einer experimentellen P-Depletion. Habilitations-schrift, Tierärztliche Hochschule Hannover.Google Scholar
Breves, G., Beyerbach, M., Höller, H. & Lessmann, H.W. (1985). Flüssigkeitsumsetzungen im Pansen von Schafen bei niedriger und ausreichender Phosphorversorgung. Deutsche Tierärztliche Wochenschrift 92, 47–49.Google Scholar
Breves, G. & Höller, H. (1983). Protein digestion in P-depleted sheep. Proceedings of the 4th International Symposium on Protein Metabolism and Nutrition at Clermont-Ferrand, EAAP-Publication No. 31, Ed. INRA Publication, pp. 321–324.Google Scholar
Breves, G.Höller, H. & Lessmann, H.W. (1985). Turnover of microbial nitrogen in the rumen of phosphorus-depleted sheep. Proceedings of the Nutrition Society 44, 145A.Google Scholar
Brüggemann, E. (1984). Untersuchungen an wachsenden Ratten zum Einfluβ der Energie- und Proteinversorgung und des kompensatorischen Wachstums auf den Proteinumschlag. Ph.D. thesis, University of Bonn.Google Scholar
Durand, , Michelle, , Beaumatin, P. & Dumay, C. (1983). Estimation in vitro à l'aide du phosphore radioactif des besoins en phosphore des microorganismes dur rumen. Reproduction, Nutrition, Developpement 23, 737—739.Google Scholar
Durand, , Michelle, , Boxebeld, A., Dumay, C. & Beaumatin, P. (1983). Influence of the level of dietary phosphorus on urea utilization by rumen microorganisms in lambs. Proceedings of the 4th International Symposium on Protein Metabolism and Nutrition at Clermont-Ferrand, EAAP-Publication No. 31, Ed. INRA Publication, pp. 263–266.Google Scholar
Durand, , Michelle, & Kawashima, R. (1980). Influence of minerals in microbial digestion. In Digestive Physiology and Metabolism in Ruminants (ed. Ruckebusch, Y. & Thivend, P., pp. 375–408. Lancaster: MTP-Press.Google Scholar
Farries, F.E. & Krasnodebska, I. (1972). Zum Einfluβ unterschiedlicher P-Versorgung auf den N-Stoffwechsel bei ausschlieβlicher NPN-Zufuhr. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 30, 33–47.Google Scholar
Faust, H. (1965). Zur Probenchemie von Stickstoffverbindungen für die emissionsspektrometrische Isotopenanalyse des Stickstoffs. Isotopenpraxis 1, 62–65.Google Scholar
Fenster, R. & Pfeffer, E. (1982). Untersuchungen zum N-Umsatz bei weiblichen Ziegen unter Verwendung von15N. II. Synthese und Abbau von Körperprotein während der Laktation. Zeitschrift für Tierphysiologie, Tierernährung und Futtermittelkunde 48, 168–175.Google Scholar
Höller, H., Breves, G. & Martens, H. (1983). Non-protein nitrogen and reduced phosphorus supply to sheep. Proceedings of the Symposium ‘Nuclear Techniques for Assessing and Improving Ruminant Feeds’ pp. 81–87. Vienna: International Atomic Energy Agency.Google Scholar
Hornung, M. (1983). Untersuchungen zum diätetischen Phosphormangel bei Schafen. Ph.D. thesis, Tierärztliche Hochschule Hannover.Google Scholar
Komisarczuk, S., Merry, R.J., McAllan, A.D., Smith, R.H. & Durand, , Michelle, (1984). Use of a continuous culture system to study phosphorus requirements in the rumen. Canadian Journal of Animal Science 64, (Suppl.) 35–36.Google Scholar
Lessmann, H.W. (1985). Der Einfluβ einer diätetischen Phophor-Depletion auf den Nettozuwachs an mikrobiell gebundenem Stickstoff im Pansen von Schafen. Ph.D. thesis, Tierärztliche Hochschule Hannover.Google Scholar
Milton, J.T.B. & Ternouth, J.H. (1984). The effects of phosphorus upon in vitro microbial digestion. Proceedings of the Australian Society of Animal Production 15, 472–475.Google Scholar
Müschen, H., Petri, A., Breves, G. & Pfeffer, E. (1988). Response of lactating goats to low phosphorus intake. 1. Milk yield and faecal excretion of P and Ca. Journal of Agricultural Science, Cambridge 111, 000–000.CrossRefGoogle Scholar
Nolan, J.V. & Leng, R.A. (1972). Dynamic aspects of ammonia and urea metabolism in sheep. British Journal of Nutrition 27, 177–194.Google Scholar
Nolan, J.V. & Leng, R.A. (1974). Isotope techniques for studying the dynamics of nitrogen metabolism in ruminants. Proceedings of the Nutrition Society 33, 1–8.CrossRefGoogle ScholarPubMed
Nolan, J.V. & Leng, R.A. (1983). Nitrogen metabolism in the rumen and its measurements. Proceedings of the Symposium ‘Nuclear Techniques for Assessing and Improving Ruminant Feeds’, pp. 43–65. Vienna: International Atomic Energy Agency.Google Scholar
Nolan, J.V. & Stachiw, S. (1979). Fermentation and nitrogen dynamics in Merino sheep given allow-qualityroughage diet. British Journal of Nutrition 42, 63–80.Google Scholar
Oldham, J.D. (1983). Patterns of nutrient utilization. Implications for nitrogen metabolism. Proceedings of the Symposium ‘Nuclear Techniques for Assessing and Improving Ruminant Feeds’, pp. 105–124. Vienna: International Atomic Energy Agency.Google Scholar
Petri, A. (1987). Quantifizierung von N-Umsetzungen in laktierenden Ziegen mittels intraruminaler Dauerinfusion von 15N-Ammoniumsalzen bei angemessener und mangelhafter Phosphor-Versorgung. Ph.D. thesis, University of Bonn.Google Scholar
Petri, A. & Pfeffer, E. (1987). Changes of 15N enrichment in N of rumen ammonia, rumen bacteria and milk protein during and following continuous intraruminal infusion of 15NH4C1 to goats. Journal of Animal Physiology and Animal Nutrition 57, 75–82.Google Scholar
Siddons, R.C., Nolan, J.V., Beever, D.E. & Macrae, J.C. (1985). Nitrogen digestion and metabolism in sheep consuming diets containing contrasting forms and levels of N. British Journal of Nutrition 54, 175–187.Google Scholar
Wetzel, K., Hübner, G. & Gebhardt, O. (1963). Untersuchungen zum N-Stoffwechsel beim laktierenden Rind unter Verwendung von oral verabreichtem Ammoniumbicarbonat-15N. Archiv für Tierernährung 13, 424–442.Google Scholar