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Rapid, plant-conserving estimation of heat tolerance in plants

Published online by Cambridge University Press:  27 March 2009

I. C. Onwueme
I. C. Onwueme
Affiliation:
Plant Science Department, University of Ife, Ile-Ife, Nigeria
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Summary

Two methods were developed for the rapid estimation of heat tolerance in plants using excised tissue pieces. The first method was a modification of the conductivity-bridge method and could yield results in less than 3 h. The second method combined plasmolysis with vital staining for the estimation of tissue injury following a regulated heat stress. This method was first developed and perfected using onion bulb epidermis tissue. It was later adapted for estimation of heat tolerance in intact tomato plants. Results from this method could be obtained within 1 h. The advantages and limitations of the two methods are compared and discussed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 92 , Issue 3 , June 1979 , pp. 527 - 535
Copyright
Copyright © Cambridge University Press 1979

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