Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-25T05:47:50.697Z Has data issue: false hasContentIssue false
  • English
  • Français

Physiological and transcriptional responses of broad bean (Vicia faba L.) leaves to aluminium stress

Published online by Cambridge University Press:  22 August 2013

H. N. XU ,
K. WANG ,
Y. N. ZHANG ,
Q. CHEN ,
L. M. CHEN  and
K. Z. LI
H. N. XU
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
K. WANG
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
Y. N. ZHANG
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
Q. CHEN
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
L. M. CHEN
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
K. Z. LI*
Affiliation:
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, 650500, People's Republic of China
*
*To whom all correspondence may be addressed. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Aluminium (Al) toxicity is the major factor-limiting crop productivity in acid soils. In the present study, physiological and transcriptional responses of broad bean leaves to Al stress were investigated. Malondialdehyde (MDA) content, H2O2 content and protein carbonyls (PC) level in leaves were increased after 100 μm AlCl3 stress treatment, whereas the total protein content was decreased, compared with the plants without Al treatment. Stomatal closure in leaves of broad bean was increased after Al stress, suggesting that the photosynthesis rate might be affected by Al stress. The relative citrate secretion in leaves was decreased after Al treatment for 24 h according to the 13C-NMR analysis, indicating that citrate in leaves might be transported to the root to chelate Al3+. To investigate the molecular mechanisms of Al toxicity in leaves of broad bean, a suppression subtractive hybridization (SSH) library was constructed to identify up-regulated genes: cDNA from leaves subjected to 12, 24, 48 and 72 h of 50 and 100 μm AlCl3 stress were used as testers and cDNA from leaves subjected to 0 μm AlCl3 treatment for the same lengths of time as above were used as a driver. The SSH analysis identified 156 non-redundant putative Al stress-responsive expressed sequence tags (ESTs) out of 960 clones. The ESTs were categorized into ten functional groups, which were involved in metabolism (0·21), protein synthesis and protein fate (0·10), photosynthesis and chloroplast structure (0·09), transporter (0·08), cell wall related (0·06), signal transduction (0·05), defence, stress and cell death (0·05), energy (0·03), transcription factor (0·03) and unknown proteins (0·30). The effect of Al treatment on expression of 15 selected genes was investigated by reverse transcription polymerase chain reaction (RT–PCR), confirming induction by Al stress. The results indicated that genes involved in organic acid metabolism, transport, photosynthesis and chloroplast structure, defence, stress and cell death might play important roles under Al stress.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 6 , December 2014 , pp. 894 - 905
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aftab, T., Khan, M. M. A., Idrees, M., Naeem, M. & Moinuddin, (2010). Effects of aluminium exposures on growth, photosynthetic efficiency, lipid peroxidation, antioxidant enzymes and artemisinin content of Artemisia annua L. Journal of Phytology 2, 23–37.Google Scholar
Basha, E., Lee, G. J., Demeler, B. & Vierling, E. (2004). Chaperone activity of cytosolic small heat shock proteins from wheat. European Journal of Biochemistry 271, 1426–1436.Google Scholar
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.Google Scholar
Chen, L. M., Yurimoto, H., Li, K. Z., Orita, I., Akita, M., Kato, N., Sakai, Y. & Izui, K. (2010). Assimilation of formaldehyde in transgenic plants due to the introduction of the bacterial ribulose monophosphate pathway genes. Bioscience, Biotechnology and Biochemistry 74, 627–635.Google Scholar
Chen, L. S. (2006). Physiological responses and tolerance of plant shoot to aluminum toxicity. Journal of Plant Physiology and Molecular Biology 32, 143–155.Google Scholar
Chen, Q., Zhang, X. D., Wang, S. S., Wang, Q. F., Wang, G. Q., Nian, H. J., Li, K. Z., Yu, Y. X. & Chen, L. M. (2011). Transcriptional and physiological changes of alfalfa in response to aluminium stress. Journal of Agricultural Science, Cambridge 149, 737–751.Google Scholar
Chen, Q., Wu, K. H., Zhang, Y. N., Phan, X. H., Li, K. Z., Yu, Y. X. & Chen, L. M. (2012). Physiological and molecular responses of broad bean (Vicia faba L.) to aluminum stress. Acta Physiologiae Plantarum 34, 2251–2263.CrossRefGoogle Scholar
Dong, B., Sang, W. L., Jiang, X., Zhou, J. M., Kong, F. X., Hu, W. & Wang, L. S. (2002). Effects of aluminum on physiological metabolism and antioxidant system of wheat (Triticum aestivum L.). Chemosphere 47, 87–92.CrossRefGoogle ScholarPubMed
Duc, G., Bao, S., Baum, M., Redden, B., Sadiki, M., Suso, M. J., Vishniakova, M. & Zong, X. (2010). Diversity maintenance and use of Vicia faba L. genetic resources. Field Crops Research 115, 270–278.Google Scholar
Gurel, A., Coskun, O., Armutcu, F., Kanter, M. & Ozen, O. A. (2005). Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: biochemical and histological studies. Journal of Chemical Neuroanatomy 29, 173–178.Google Scholar
He, G. H., Zhang, J. F., Hu, X. H. & Wu, J. C. (2011). Effect of aluminum toxicity and phosphorus deficiency on the growth and photosynthesis of oil tea (Camellia oleifera Abel.) seedlings in acidic red soils. Acta Physiologiae Plantarum 33, 1285–1292.CrossRefGoogle Scholar
Hoekenga, O. A. & Magalhaes, J. V. (2011). Mechanisms of aluminum tolerance. In Root Genomics (Eds Costa de Oliveira, A. & Varshney, R. K.), pp. 133–153. Heidelberg, Germany: Springer-Verlag.Google Scholar
Inostroza-Blancheteau, C., Rengel, Z., Alberdi, M., De La Luz Mora, M., Aquea, F., Arce-Johnson, P. & Reyes-Diaz, M. (2011). Molecular and physiological strategies to increase aluminium resistance in plants. Molecular Biology Reports 39, 2069–2079.Google Scholar
Jiang, H. X., Chen, L. S., Zheng, J. G., Han, S., Tang, N. & Smith, B. R. (2008). Aluminum-induced effects on Photosystem II photochemistry in C itrus leaves assessed by the chlorophyll a fluorescence transient. Tree Physiology 28, 1863–1871.Google Scholar
Kochian, L. V. (1995). Cellular mechanisms of Al toxicity and resistance in plants. Annual Review of Plant Physiology and Plant Molecular Biology 46, 237–260.Google Scholar
Law, R. D., Crafts-Brandner, S. J. & Salvucci, M. E. (2001). Heat stress induces the synthesis of a new form of ribulose-1,5- bisphosphate carboxylase/oxygenase activase in cotton leaves. Planta 214, 117–125.Google Scholar
Li, L., Li, S. M., Sun, J. H., Zhou, L. L., Bao, X. G., Zhang, H. G. & Zhang, F. S. (2007). Diversity enhances agricultural productivity via rhizosphere phosphorus facilitation on phosphorus-deficient soils. Proceedings of the National Academy of Sciences of the United States of America 104, 11192–11196.Google Scholar
Liu, Q., Zheng, S. & Lin, X. (2004). Plant physiological and molecular biological mechanism in response to aluminium toxicity. Chinese Journal of Applied Ecology 15, 1641–1649.Google Scholar
Ma, Y., Yang, T., Guan, J., Wang, S., Wang, H., Sun, X. & Zong, X. (2011). Development and characterization of 21 EST-derived microsatellite markers in Vicia faba (faba bean). American Journal of Botany 98, 22–24.Google Scholar
Madhava Rao, K. V. & Sresty, T. V. S. (2000). Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science 157, 113–128.Google Scholar
Merlot, S., Leonhardt, N., Fenzi, F., Valon, C., Costa, M., Piette, L., Vavasseur, A., Genty, B., Boivin, K., MüLLER, A., Giraudat, J. & Leung, J. (2007). Constitutive activation of a plasma membrane H+-ATPase prevents abscisic acid-mediated stomatal closure. EMBO Journal 26, 3216–3226.Google Scholar
Milla, M. A. R., Butler, E., Huete, A. R., Wilson, C. F., Anderson, O. & Gustafson, J. P. (2002). Expressed sequence tag-based gene expression analysis under aluminum stress in rye. Plant Physiology 130, 1706–1716.CrossRefGoogle ScholarPubMed
Panda, S. K. & Matsumoto, H. (2010). Changes in antioxidant gene expression and induction of oxidative stress in pea (Pisum sativum L.) under Al stress. Biometals 23, 753–762.Google Scholar
Panda, S. K., Baluska, F. & Matsumoto, H. (2009). Aluminum stress signaling in plants. Plant Signaling and Behavior 4, 592–597.Google Scholar
Patterson, B. D., Macrae, E. A. & Ferguson, I. B. (1984). Estimation of hydrogen peroxide in plant extracts using Titanium (IV). Analytical Biochemistry 139, 487–492.Google Scholar
Richards, K. D., Schott, E. J., Sharma, Y. K., Davis, K. R. & Gardner, R. C. (1998). Aluminum induces oxidative stress genes in Arabidopsis thaliana . Plant Physiology 116, 409–418.Google Scholar
Rubiales, D. (2010). Faba beans in sustainable agriculture. Field Crops Research 115, 201–202.Google Scholar
Samac, D. A. & Tesfaye, M. (2003). Plant improvement for tolerance to aluminum in acid soils – a review. Plant Cell, Tissue and Organ Culture 75, 189–207.Google Scholar
Silva, S., Pinto, G., Dias, M. C., Correia, C. M., Moutinho-Pereira, J., Pinto-Carnide, O. & Santos, C. (2012). Aluminium long-term stress differently affects photosynthesis in rye genotypes. Plant Physiology and Biochemistry 54, 105–112.Google Scholar
Tamás, L., Huttová, J., Mistrík, I., Simonovicová, M. & Siroka, B. (2003). Aluminium-induced drought and oxidative stress in barley roots. Journal of Plant Physiology 163, 781–784.Google Scholar
Tesfaye, M., Temple, S. J., Allan, D. L., Vance, C. P. & Samac, D. A. (2001). Overexpression of malate dehydrogenase in trangenic alfalfa enhances organic acid synthesis and confers tolerance to aluminium. Plant Physiology 127, 1836–1844.Google Scholar
Zhang, H., Zhang, S., Meng, Q., Zou, J., Jiang, W. S. & Liu, D. (2009). Effects of aluminum on nucleoli in root tip cells, root growth and the antioxidant defence system in Vicia faba L. Acta Biologica Cracoviensia Series Botanica 51, 99–106.Google Scholar
Zhou, L. L., Bai, G. H., Carver, B. & Zhang, D. D. (2007). Identification of new sources of aluminum resistance in wheat. Plant and Soil 297, 105–118.Google Scholar
Zhou, S. P., Sauvé, R. & Thannhauser, T. W. (2009). Proteome changes induced by aluminium stress in tomato roots. Journal of Experimental Botany 60, 1849–1857.Google Scholar