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Nutritional evaluation of kale (Brassica oleracea) diets:2. Copper deficiency, thyroid function, and selenium status in young cattle and sheep fed kale for prolonged periods

Published online by Cambridge University Press:  27 March 2009

T. N. Barry ,
T. C. Reid ,
K. R. Millar  and
W. A. Sadler
T. N. Barry
Affiliation:
Invermay Agricultural Research Centre, Ministry of Agriculture and Fisheries, Mosgiel, New Zealand
T. C. Reid
Affiliation:
Invermay Agricultural Research Centre, Ministry of Agriculture and Fisheries, Mosgiel, New Zealand
K. R. Millar
Affiliation:
Wallaceville Research Centre, Ministry of Agriculture and Fisheries, Wellington, New Zealand
W. A. Sadler
Affiliation:
Department of Nuclear Medicine, Christchurch Hospital, New Zealand
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Summary

Animals fed sole diets of kale (Brassica oleracea) were compared with animals fed ryegrass-clover pasture grown on the same soil type in two experiments. In Expt 1 young cattle grazed the two forages for 24 weeks, with supplementary copper and iodine being administered by injection. In Expt 2 young sheep were individually fed the two forages indoors at equal D.m. intake.

Animals grazing kale in Expt 1 showed the characteristic symptoms of haemolytio anaemia from ruminal fermentation of S-methyl cysteine sulphoxide (SMCO) (Smith, 1974). This was most severe over the first 6 weeks, during which live-weight gains were very low (250 g/day). In the absence of copper supplementation animals grazing kale showed symptoms of copper deficiency. This was characterized by live-weight gain remaining low throughout the experiment (mean 280 g/day), rapid depletion of liver copper reserves, progressive reductions in serum copper concentration, reductions in erythrocyte copper and reduced glutathione (GSH) concentrations and a massive hepatic accumulation of iron. Copper deficiency only slightly lowered heart muscle copper concentration in kale-fed cattle, and this was counteracted byheart hypertrophy. The major effects of copper deficiency in kale-fed cattle were in erythrocytes, and a metabolic diagram is presented showing these effects to be biochemically similar to those produced by ruminal fermentation of SMCO.

Copper supplementation of animals grazing kale increased live-weight gain (mean 425 g/day), reduced Heinz body formation, allowed the animals to recover gradually from the haemolytic anaemia and prevented other symptoms of copper deficiency. In contrast, animals grazing ryegrass-clover pasture showed only a very mild depletion of copper, there being no response in live-weight gain to copper supplementation.

Activity of the enzyme glutathione peroxidase (GSH-Px) in whole blood was dependent upon blood selenium concentration in cattle fed both diets. In cattle fed on kale, bub not on pasture, reductions in erythrocyte GSH due to ruminal fermentation of SMCO and to copper deficiency were also associated with depressed blood selenium status.

Glucosinolates were present in the kale (11μM/g D.M.) but absent from the pasture diet. Despite this, neither T4 production from the thyroid gland nor the conversion of T4 to T3 appeared to be impaired by kale feeding in either Expt 1 or Expt 2. In Expt 1 serum T3 concentration was better relatedto live-weight gain than was serum T4 concentration, in accord with T3 being the active form of the thyroid hormone.

It is concluded that supplementation with copper but not iodine is essential where growing cattle are fed sole diets of kale for periods in excess of 12 weeks

Type
Research Article
Information
The Journal of Agricultural Science , Volume 96 , Issue 2 , April 1981 , pp. 269 - 282
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Abdullah, R. & Falconer, I. R. (1977). Responses of thyroid activity to feed restriction in the goat. Australian Journal of Biological Sciences 30, 207–215.CrossRefGoogle ScholarPubMed
Allcroft, R. & Uvarov, O. (1959). Parental administration of copper compounds to cattle with special reference to copper glycine. Veterinary Record 71, 797–810.Google Scholar
Andrewartha, K. A. & Caple, I. W. (1980). Effeot of changes in nutritional copper on erythrocyte superoxide dismutase activity in sheep. Research in Veterinary Science 28, 101–104.Google Scholar
Barry, T. N., McDonald, R. C. & Reid, T. C. (1981). Nutritional evaluation of kale (Brassica oleracea) diets. 1. Growth of grazing lambs as affected by time after introduction to the crop, feed allowance and intraperitoneal amino acid supplementation. Journal of Agricultural Science, Cambridge 96, 257–267.CrossRefGoogle Scholar
Barry, T. N., Manley, T. R., Redekopp, C., Davis, S. R., Fairclough, R. & Lapwood, K. (1981). Protein metabolism in growing lambs fed fresh ryegrass/clover pasture ad libitum. 2. Glucose production and changes in the endocrine system in response to abomasal infusion of casein + mothionine. British Journal of Nutrition (in the Press).Google Scholar
Bernal, J. & Refetoff, S. (1977). The action of the thyroid hormone. Clinical Endocrinology 6, 227–249.Google Scholar
Beutler, E., Duran, O. & Kelly, B. M. (1963). Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine 61, 882–888.Google Scholar
Clark, F. (1963). Resin uptake of 181 I triiodothyronine; an in vitro test of thyroid function. The Lancet No. 7300, 167–169.Google Scholar
Clark, F. & Horn, D. B. (1965). Assessment of thyroid. function by the combined use of the serum proteinbound iodïde and resin uptake of 131 I triiodothyronine. Journal of Clinical Endocrinology 25, 39–45.Google Scholar
Drew, K. R. (1966). The in vitro prediction of herbage digestibility. Proceedings of the New Zealand Society of Animal Production 26, 52–70.Google Scholar
Ferguson, K. A., Wallace, A. L. C. & Lindner, H. R. (1965). Hormonal regulation of wool growth. In Biology of the Skin and Hair Growth (ed.Lyn, A. G. & Short, B. F.), pp. 655–677. Sydney: Angus& Robertson.Google Scholar
Flanjak, J. & Lee, H. Y. (1979). Trace metal contents of livers and kidneys of cattle. Journal of the Science of Food and Agriculture 30, 503–507.Google Scholar
Greenhalgh, J. F. D. (1968). Kale anaemia. Proceedings of the Nutrition Society 29, 178–183.Google Scholar
Ingbar, S. H. & Braverman, L. E. (1975). Active form of the thyroid hormone. Annual Reviews of Medicine 26, 443–449.Google Scholar
John, A., Ulyatt, M. J., Jones, W. T. & Shelton, I. D. (1980). Effects of feed protein solubility on its digestion and utilisation by sheep. Proceedings New Zealand Society of Animal Production (in the Press).Google Scholar
Mills, C. F. & Daloarno, A. C. (1970). An evaluation of tissue cytochrome oxidase activity as an indicator of copper status. In Trace Element Metabolism in Animals (ed. Mills, C. F.). London: Livingstones.Google Scholar
Miska, H. P. & Fridovich, I. (1972). The generation of superoxide radical during the autoxidation of haemoglobin. Journal of Biological Chemistry 247, 6960–6962.Google Scholar
Moxon, R. E. D. & Dixon, E. J. (1980). Semi-automatic method for the determination of total iodine in foods. Analyst 105, 344–352.Google Scholar
Paglia, D. E. & Valentine, W. N. (1967). Studies on the quantitative and qualitative characteristics of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158–169.Google Scholar
Rotilio, G., Rigo, A., Bracci, R., Bagnoli, F., Sargentini, I. & Brunori, M. (1977). Determination of red blood cell superoxide dismutase and glutathione peroxidase in new-boms in relation to neonatal haemolysis. Clinica Chimica Acta 81, 131–134.Google Scholar
Sadler, W. A. & Brownlie, B. E. W. (1975). Triiodothyronine radio-immunoassay in the assessment of thyroid function. New Zealand Medical Journal 81, 328–334.Google Scholar
Sinclair, D. P. & Andrews, E. D. (1961). Deaths due to goitre in new-born lambs prevented by iodized poppy-seed oil. New Zealand Veterinary Journal 9, 96–100.Google Scholar
Smith, R. H. (1974). Kale poisoning. Report Rowett Institute 30, 112–131.Google Scholar
Smith, R. H. (1978). S-methyl cysteine sulphoxide, the brassica anaemia factor (a valuable dietary factor forman?). Veterinary Science Communications 2, 47–61.CrossRefGoogle Scholar
Suttle, N. F. (1976). The detection and prevention of trace deficiencies in animal husbandry systems. Chemistry and Industry 559–562.Google Scholar
Suttle, N. F. & McLauchlan, M. (1976). Predicting the effects of dietary molybdenum and sulphur on the availability of copper to ruminants. Proceedings of the Nutrition Society 35, 22A.Google Scholar
Tapper, B. A. & Reay, P. F. (1973). Cyanogenio glycosides and glucosinolates. In Chemistry and Biochemistry of Herbage, Vol. 1 (ed. Butler, G. W. and Bailey, R. W.), pp. 468–470. London and New York: Academic Press.Google Scholar
Underwood, E. J. (1977).Trace Elements in Human & Animal Nutrition. New York: Academic Press.Google Scholar
Van Etten, C. H. & Daxenbichler, M. E. (1977). Glucosinolates and derived products in Cruciferous vegetables: total glucosinolates by retention on anion exchange resin and enzymic hydrolysis to measure released glucose. Journal Association Official Analytical Chemists 60, 946–953.Google Scholar
Watkinson, J. (1966). Fluorometric determination of selenium in biological material with 2,3-diaminonaphthelene. Analytical Chemistry 38, 92–97.Google Scholar
Webster, A. J. F. (1976). In Principles of Cattle Production (ed. Swan, H. and Broster, W. H.), p. 19. London: Butterworths.Google Scholar
Wever, R., Oudeoa, B. & Van Gelder, B. F. (1973). Generation of superoxide radicals during the autoxidation of mammalian oxyhaemoglobin. Biochemica et Biophysica Acta 302, 475–478.Google Scholar