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Nonidet p-40, a novel inducer, activates cucumber disease resistance against cucumber anthracnose disease

Published online by Cambridge University Press:  07 October 2013

T. C. LIN ,
C. L. LIN  and
J. W. HUANG
T. C. LIN
Affiliation:
Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan
C. L. LIN
Affiliation:
Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan
J. W. HUANG*
Affiliation:
Department of Plant Pathology, National Chung Hsing University, Taichung 402, Taiwan
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

The present study found that a protein lysis buffer, used for the extraction of proteins from cells, showed efficacy in reducing the disease severity of cucumber anthracnose, which is caused by the anthracnose fungus. The lysis buffer and its individual components were examined for their function in reducing disease development of cucumber anthracnose on cucumber plants. Nonidet P-40, a nonionic detergent commonly used to isolate cell membrane complexes, was the most effective component of the lysis buffer for disease control. The treatment of cucumber plants with Nonidet P-40 at a concentration of 50 μl/l suppressed development of cucumber anthracnose, but it did not inhibit spore germination of the fungus. Cucumber plants were inoculated with the pathogen 30 min after treatment with Nonidet P-40, and a reduction in disease severity was observed. Expression of genes related to disease resistance (acidic class III chitinase, phenylalanine ammonialyase 1, peroxidase and pathogenesis-related protein 1-1a) were also examined after plants were treated with Nonidet P-40 and inoculated with the pathogen. The results indicate that Nonidet P-40 functions as a trigger for a stereotypic defence response in cucumber plants, including an increase in the expression levels of genes related to disease resistance.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 6 , December 2014 , pp. 932 - 940
Copyright
Copyright © Cambridge University Press 2013 

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References

Arlat, M., Van Gijsegem, F., Huet, J. C., Pernollet, J. C. & Boucher, C. A. (1994). PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum . EMBO Journal 13, 543–553.Google Scholar
Ashani, Y. & Catrovas, G. N. (1980). Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Analytical Biochemistry 109, 55–62.Google Scholar
Baillieul, F., Genetet, I., Kopp, M., Saindrenan, P., Fritig, B. & Kauffmann, S. (1995). A new elicitor of the hypersensitive response in tobacco: a fungal glycoprotein elicits cell death, expression of defence genes, production of salicylic acid, and induction of systemic acquired resistance. Plant Journal 8, 551–560.Google Scholar
Boller, T. & Felix, G. (2009). A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors. Annual Review of Plant Biology 60, 379–406.Google Scholar
Cools, H. J. & Ishii, H. (2002). Pre-treatment of cucumber plants with acibenzolar-S-methyl systemically primes a phenylalanine ammonia lyase gene (PAL1) for enhanced expression upon attack with a pathogenic fungus. Physiological and Molecular Plant Pathology 61, 273–280.CrossRefGoogle Scholar
Davies, A. A., Wigglesworth, N. M., Allan, D., Owens, R. J. & Crumpton, M. J. (1984). Nonidet P-40 extraction of lymphocyte plasma membrane. Characterization of the insoluble residue. Biochemical Journal 219, 301–308.Google Scholar
He, S. Y., Huang, H. C. & Collmer, A. (1993). Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell 73, 1255–1266.CrossRefGoogle ScholarPubMed
Hronská, L., Mrózová, Z., Valachovič, M. & Hapala, I. (2004). Low concentrations of the non-ionic detergent Nonidet P-40 interfere with sterol biogenesis and viability of the yeast Saccharomyces cerevisiae . FEMS Microbiology Letters 238, 241–248.Google Scholar
Jaeger, J., Sorensen, K. & Wolff, S. P. (1994). Peroxide accumulation in detergents. Journal of Biochemical and Biophysical Methods 29, 77–81.CrossRefGoogle ScholarPubMed
Kawahara, T., Namba, H., Toyoda, K., Kasai, T., Sugimoto, M., Inagaki, Y., Ichinose, Y. & Shiraishi, T. (2006). Induction of defense responses in pea tissues by inorganic phosphate. Journal of General Plant Pathology 72, 129–136.Google Scholar
Lin, T. C. & Ishii, H. (2009). Accumulation of H2O2 in xylem fluids of cucumber stems during ASM-induced systemic acquired resistance involves increased LOX activity and transient accumulation of shikimic acid. European Journal of Plant Pathology 125, 119–130.Google Scholar
Mao, J., Liu, Q., Yang, X., Long, C., Zhao, M., Zeng, H., Liu, H., Yuan, J. & Qiu, D. (2010). Purification and expression of a protein elicitor from Alternaria tenuissima and elicitor-mediated defence responses in tobacco. Annals of Applied Biology 156, 411–420.Google Scholar
Moskvina, E., Imre, E.-M. & Ruis, H. (1999). Stress factors acting at the level of the plasma membrane induce transcription via the stress response element (STRE) of the yeast Saccharomyces cerevisiae . Molecular Microbiology 32, 1263–1272.Google Scholar
Narusaka, Y., Narusaka, M., Horio, T. & Ishii, H. (1999). Induction of disease resistance in cucumber by acibenzolar-S-methyl and expression of resistance-related genes. Annals of the Phytopathological Society of Japan 65, 116–122.Google Scholar
Paré, P. W., Farag, M. A., Krishnamachari, V., Zhang, H., Ryu, C. M. & Kloepper, J. W. (2005). Elicitors and priming agents initiate plant defense responses. Photosynthesis Research 85, 149–159.Google Scholar
Peng, D., Qiu, D., Ruan, L., Zhou, C. & Sun, M. (2011). Protein elicitor pemG1 from Magnaporthe grisea induces systemic acquired resistance (SAR) in plants. Molecular Plant-Microbe Interactions 24, 1239–1246.Google Scholar
Sato, T., Kubo, M. & Watanabe, S. (2003). Heat shock induces a systemic acquired resistance (SAR)-related gene via salicylic acid accumulation in cucumber (Cucumis sativus L.). Japanese Journal of Tropical Agriculture 47, 77–82.Google Scholar
Sharathchandra, R. G., Geetha, N. P., Amruthesh, K. N., Kini, K. R., Sarosh, B. R., Shetty, N. P. & Shetty, H. S. (2006). Isolation and characterisation of a protein elicitor from Sclerospora graminicola and elicitor-mediated induction of defence responses in cultured cells of Pennisetum glaucum . Functional Plant Biology 33, 267–278.Google Scholar
Strobel, N. E., Ji, C., Gopalan, S., Kuc, J. A. & He, S. Y. (1996). Induction of systemic acquired resistance in cucumber by Pseudomonas syringae pv. syringae 61 HrpZPss protein. Plant Journal 9, 431–439.Google Scholar
Veit, S., Wörle, J. M., Nürnberger, T., Koch, W. & Seitz, H. U. (2001). A novel protein elicitor (PaNie) from Pythium aphanidermatum induces multiple defense responses in carrot, Arabidopsis, and tobacco. Plant Physiology 127, 832–841.Google Scholar
Wei, Z. M., Laby, R. J., Zumoff, C. H., Bauer, D. W., He, S. Y., Collmer, A. & Beer, S. V. (1992). Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora . Science 257, 85–88.Google Scholar
Zhang, Y., Yang, X., Liu, Q., Qiu, D., Zhang, Y., Zeng, H., Yuan, J. & Mao, J. (2010). Purification of novel protein elicitor from Botrytis cinerea that induces disease resistance and drought tolerance in plants. Microbiological Research 165, 142–151.CrossRefGoogle ScholarPubMed
Zhao, J., Davis, L. C. & Verpoorte, R. (2005). Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances 23, 283–333.Google Scholar