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Manganese-resistant mutants of Azospirillum brasilense: their response of associative nitrogen fixation, dry-matter production and nitrogen content of cheena (Panicum miliaceum L.) to different amounts of applied nitrogen in acid soil

Published online by Cambridge University Press:  27 March 2009

R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi, Muzaffarpur-84312, Bihar, India

Summary

Six nitrosoguanidine-induced manganese-resistant mutants of Azospirillum brasilense were isolated and used for associative N2 fixation studies with cheena (Panicum miliaceum L.) in acid soil. Three mutants showed cross-resistance to aluminium and neomycin, but of these three only one showed cross-resistance to streptomycin. Inoculation of cheena with A. brasilense and its mutants at different rates of applied nitrogen led to a significant increase in associative N2 fixation, N content, dry-matter production and grain yield in acid soil. A saving of about 20 kg N/ha could be achieved by inoculating seeds of cheena with these mutant strains in acid soil.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1988

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