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Lysine and nitrogen measurement by infra-red reflectance analysis as an aid to barley breeding

Published online by Cambridge University Press:  27 March 2009

A. A. Gill ,
Carol Starr  and
D. B. Smith
A. A. Gill
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
Carol Starr
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
D. B. Smith
Affiliation:
Chemistry Department, Plant Breeding Institute, Trumpington, Cambridge GB2 2LQ
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Summary

The feasibility of measuring lysine and protein nitrogen in barley using an infra-red reflectance analyser was examined. This is a very rapid technique, requiring no manipulative skills, and measuring several components simultaneously using milled grains. The results show that this instrument is sensitive to changes in lysine and protein nitrogen and can be calibrated to measure these components to a high degree of accuracy. It is shown that the gross morphological and biochemical abnormalities of high lysine mutants do not affect these measurements, indicating that this method is suitable for the rapid screening of material from breeding programmes designed to improve the lysine content of barley proteins.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 93 , Issue 3 , December 1979 , pp. 727 - 733
Copyright
Copyright © Cambridge University Press 1979

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