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The isolation of plant cell wall preparations with low nitrogen contents

Published online by Cambridge University Press:  27 March 2009

I. M. Morrison
I. M. Morrison
Affiliation:
Harmah Research Institute, Ayr Ka& 5Hl
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Summary

Grasses of widely differing maturity have been extracted by two chemical and two enzymic methods in order to prepare cell wall materials with low nitrogen contents. These methods involved extraction, with neutral detergent or phenol-acetic acid-water (1:1:1), or digestion with the proteolytic enzymes pepsin or pronase. Wide differences were noted between these methods with grasses at different levels of maturity, both in the nitrogen content of the residue and the actual yield of cell wall preparation. The more efficient of these methods for removing nitrogenous materials, namely neutral detergent extraction and digestion with pepsin and pronase, were also examined for their effect on the structural carbohydrate and lignin content of the cell wall. In all respects, a method involving incubation with the enzyme, pronase, gave the most satisfactory cell wall preparation.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 80 , Issue 3 , June 1973 , pp. 407 - 410
Copyright
Copyright © Cambridge University Press 1973

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