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Influence Of Aspergillus oryzae fermentation extract on the fermentation of a basal ration in the rumen simulation technique (Rusitec)

Published online by Cambridge University Press:  27 March 2009

P. P. Frumholtz ,
C. J. Newbold  and
R. J. Wallace
P. P. Frumholtz
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
C. J. Newbold
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
R. J. Wallace
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, UK
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Summary

The effects of adding a culture extract from Aspergillus oryzae (AO) to the fermentation of a basal ration were investigated using the rumen simulation technique (Rusitec). The ration consisted of hay, barley, molasses, fishmeal and a minerals/vitamins mixture, at 500, 299–5, 100, 91 and 9–5 g/kg dry matter respectively. AO eliminated the transient fall in pH that occurred following the addition of substrate to Rusitec, but had no influence on the buffering capacity of the medium. Increases in the acetate: propionate ratio and in the proportion of butyrate in the fermentation products were observed in vessels receiving AO. Ammonia concentrations increased by over 30%. The number of total viable bacteria almost doubled and the cellulolytic population increased three-fold in vessels receiving AO. Protozoal numbers were reduced by almost 45% by AO. The proportion of methane in the headspace gas collected from the vessels during the day was significantly (P < 0·01) lower in vessels receiving AO. These results demonstrate that AO has a marked effect on the microbial population and the stoichiometry of the rumen fermentation, and that these changes can be seen in an in vitro system.

Type
Review
Information
The Journal of Agricultural Science , Volume 113 , Issue 2 , October 1989 , pp. 169 - 172
Copyright
Copyright © Cambridge University Press 1989

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