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In situ and in vitro techniques for estimating degradation parameters and digestibility of diets based on maize or sorghum

Published online by Cambridge University Press:  22 April 2020

B. C. Silva Open the ORCID record for B. C. Silva [Opens in a new window] ,
M. V. C. Pacheco ,
L. A. Godoi Open the ORCID record for L. A. Godoi [Opens in a new window] ,
F. A. S. Silva ,
D. Zanetti ,
A. C. B. Menezes ,
P. Pucetti ,
S. A. Santos ,
M. F. Paulino  and
S. C. Valadares Filho
B. C. Silva*
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
M. V. C. Pacheco
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
L. A. Godoi
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
F. A. S. Silva
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
D. Zanetti
Affiliation:
Federal Institute of Education, Science and Technology of Southern Minas Gerais, Machado, Minas Gerais37750-000, Brazil
A. C. B. Menezes
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
P. Pucetti
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
S. A. Santos
Affiliation:
Department of Preventive Veterinary Medicine and Animal Production, Universidade Federal da Bahia, Salvador, BA40170-110, Brazil
M. F. Paulino
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
S. C. Valadares Filho
Affiliation:
Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, Minas Gerais36570-900, Brazil
*
Author for correspondence: B. C. Silva, E-mail: [email protected]
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Abstract

An experiment was conducted to evaluate: (1) the effects of ensiling maize or sorghum grains after reconstitution on readily soluble fraction (a), potentially degradable fraction in the rumen (b) and rate constant for degradation of b (c) of dry matter (DM), organic matter (OM) and starch (STA); and (2) an appropriate incubation time for in situ or in vitro procedures to estimate in vivo digestibility. Four rumen-cannulated Nellore bulls (body weight = 262 ± 19.6 kg) distributed in a 4 × 4 Latin square were used. Diets were based on dry ground maize (DGM); or dry ground sorghum (DGS); or reconstituted ground maize silage; or reconstituted ground sorghum silage. In vitro and in situ incubations of the individual grains and diets were simultaneously performed with in vivo digestibility. In general, reconstituted grains and diets based on reconstituted grains presented greater (P < 0.05) fraction a and lower (P < 0.05) fraction b of DM, OM and STA compared to dry grains and diets based on dry grain. However, the magnitude of response of the reconstitution and ensiling process on DM and OM degradability parameter was greater for maize than that for sorghum. Moreover, no differences (P > 0.05) were observed between DGM- and DGS-based diets for c estimates. The results suggest that the reconstitution process promotes grains protein matrix breakdown increasing STA availability. The incubation times required for in vivo digestibility estimations of DM, OM and STA are 24 h for in situ and 36 h for in vitro procedures.

Keywords

Incubation timeNelloreprolaminsreconstituted grain silagestarch
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 158 , Issue 1-2 , March 2020 , pp. 150 - 158
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Copyright
Copyright © Cambridge University Press 2020

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