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Improvement of Cercospora leaf spot and powdery mildew resistance of mungbean variety KING through marker-assisted selection

Published online by Cambridge University Press:  12 January 2022

P. Papan ,
W. Chueakhunthod ,
W. Jinagool ,
A. Tharapreuksapong ,
A. Masari ,
C. Kaewkasi ,
S. Ngampongsai ,
T. Girdthai  and
P. A. Tantasawat Open the ORCID record for P. A. Tantasawat [Opens in a new window]
P. Papan
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
W. Chueakhunthod
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
W. Jinagool
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
A. Tharapreuksapong
Affiliation:
Center for Scientific and Technological Equipment, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
A. Masari
Affiliation:
Field and Renewable Energy Crops Research Institute, 50 Phahonyothin Avenue, Chatuchak District, Bangkok 10900, Thailand
C. Kaewkasi
Affiliation:
School of Computer Engineering, Institute of Engineering, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
S. Ngampongsai
Affiliation:
Field and Renewable Energy Crops Research Institute, 50 Phahonyothin Avenue, Chatuchak District, Bangkok 10900, Thailand
T. Girdthai
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
P. A. Tantasawat*
Affiliation:
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand
*
Author for correspondence: P. A. Tantasawat, E-mail: [email protected]
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Abstract

The development of resistant mungbean varieties is one of the most efficient strategies to control major diseases such as Cercospora leaf spot (CLS) and powdery mildew (PM). The objectives of this study were to pyramid a CLS resistance gene and two PM resistance genes from the donor parent D2 into a susceptible variety KING through marker-assisted backcrossing (MABC) and to evaluate their agronomic traits and disease resistance under field conditions. Five markers linked to the resistance genes were used for foreground selection, while two marker sets [Set A containing 15 polymorphic simple sequence repeat (SSR) and expressed sequence tag-SSR (EST-SSR) markers and Set B containing 34 polymorphic inter-simple sequence repeat (ISSR) loci] were also used for background selection. Two pyramided backcross (BC) lines, namely H3 and H4, were homozygous at all five marker loci when confirmed in BC4F4 and BC4F5 generations. Their recurrent parent genome (RPG) recovery ranged from 96.4 to 100.0%, depending on the marker sets. During field evaluation, a moderate to high level of CLS and PM resistance was observed in both BC lines compared to the susceptible recurrent parent KING. One of these BC lines (H3) had all agronomic traits similar or superior to the recurrent parent KING at all environments, and had a higher yield than KING (18.0–32.0%) under CLS and PM outbreaks. This line can be developed into a new resistant mungbean variety in Thailand in the future. These results substantiate the usefulness of MABC for transferring multiple resistance genes into an elite variety.

Keywords

Field evaluationgene pyramidingrecurrent parent genome recoveryresistance geneVigna radiata (L.) Wilczek
Type
Crops and Soils Research Paper
Information
The Journal of Agricultural Science , Volume 159 , Issue 9-10 , November 2021 , pp. 676 - 687
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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