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The free- and protein-amino acids of the rumen phycomycete fungi Neocallimastix frontalis and Piromonas communis

Published online by Cambridge University Press:  27 March 2009

P. Kemp ,
D. J. Jordan  and
C. G. Orpin
P. Kemp
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT
D. J. Jordan
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT
C. G. Orpin
Affiliation:
Agricultural and Food Research Council, Institute of Animal Physiology, Babraham, Cambridge, CB2 4AT
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Summary

The proteins and the amino acid profile of the rumen fungi Neocallimastix frontalis and Piromonas communis have been examined and the in vitro digestibility of the vegetative growth determined.

The mean true protein content of N. frontalis was 24% of the dry weight and 30% for P. communis, which has a lower chitin content. 50% of the protein extracted from cells and examined by HPLC was in the 200000 MW range and 40% in the 50000 MW range. Polyacrylamide gel electrophoresis after treatment with sodium dodecylsulphate and 2-mercaptoethanol indicated that the native proteins were composed of smaller units in the MW range 25000–50000.

The amino acid profiles of the two fungi were similar and compared favourably with those of casein and fraction 1 protein of lucerne.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 105 , Issue 3 , December 1985 , pp. 523 - 526
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Kemp, P., Lander, D. & Orpin, C. G. (1984). The lipids of the rumen fungus Piromonas communis. Journal of General Microbiology 130, 27–37.Google ScholarPubMed
Mangan, J. L. (1982). The nitrogenous constituents of fresh forages. In Forage Protein in Ruminant Animal Production (ed. Thomson, D. J., Beaver, D. E. and Gunn, R. G.), pp. 25–40. British Society of Animal Production: Occasional Publication no. 6.Google Scholar
Mangan, J. L. & West, J. (1977). Ruminal digestion of chloroplasts and the protection of protein by glutaraldehyde treatment. Journal of Agricultural Science, Cambridge 89, 3–15.CrossRefGoogle Scholar
Munn, E. A., Orpin, C. G. & Hall, F. J. (1981). Ultrastructural studies of the free zoospore of the rumen phycornycete Neocallimastix frontalis. Journal of General Microbiology 125, 311–323.Google ScholarPubMed
Neill, A. R., Grime, D. W., Snoswell, A. M., Northrop, A. J., Lindsay, D. B. & Dawson, R. M. C. (1979). The low availability of dietary choline for the nutrition of sheep. Biochemical Journal 180, 559–565.CrossRefGoogle Scholar
Nugent, J. H. N. (1979). Proteolysis in the rumen. Ph.D. thesis, University of Cambridge.Google Scholar
Orpin, C. G. (1975). Studies on the rumen flagellate Neocallimastix frontalis. Journal of General Microbiology 91, 249–262.CrossRefGoogle ScholarPubMed
Orpin, C. G. (1977 a). Invasion of plant tissues by the flagellate Neocallimastix frontalis. Journal of General Microbiology 98, 423–430.CrossRefGoogle ScholarPubMed
Orpin, C. G. (1977 b). The rumen flagellate Piromonas communis: its life history and invasion of plant material in the rumen. Journal of General Microbiology 99, 107–117.CrossRefGoogle ScholarPubMed
Orpin, C. G. (1977 c). The occurrence of chitin in the cell walls of the rumen organisms Neocallimastix frontalis, Piromonas communis and Sphaeromonas communis. Journal of General Microbiology 99, 215–218.CrossRefGoogle Scholar
Orpin, C. G. (1981). Fungi in ruminant nutrition. Agricultural Science Seminar: Degradation of Plant Cell Wall Material. London: Agricultural Research Council.Google Scholar
Tilley, J. M. A. & Terry, R. A. (1963). A two stage technique for in vitro digestion of forage crops. Journal of the British Grassland Society 18, 104–111.CrossRefGoogle Scholar